3.4.21.B52: thermostable serine endopeptidase (Sulfolobus)

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For detailed information about thermostable serine endopeptidase (Sulfolobus), go to the full flat file.

Reaction

The best substrate is benzyloxycarbonyl-Tyr-4-nitroanilide. Cleaves Phe-Ser-Lys-Phe-Ile-/-Ser-Phe-Asp. No activity benzyloxycarbonyl-Ile-4-nitroanilide, aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin or ovalbumin. =

Synonyms

proteinase I

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B52 thermostable serine endopeptidase (Sulfolobus)

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Results	in table
2	General Stability
9	Inhibitors
6	kcat/KM [mM/s]
6	KM Value [mM]
3	Localization
3	Metals/Ions
3	Molecular Weight [Da]
6	Organic Solvent Stability
20	Organism
3	pH Optimum
1	pI Value
1	Purification (Commentary)
1	Reaction
3	Reference
1	Specific Activity [micromol/min/mg]
11	Substrates and Products (Substrate)
1	Subunits
2	Synonyms
1	Temperature Optimum [°C]
5	Temperature Stability [°C]
6	Turnover Number [1/s]

Reaction

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Reaction on EC 3.4.21.B52 - thermostable serine endopeptidase (Sulfolobus)

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

The best substrate is benzyloxycarbonyl-Tyr-4-nitroanilide. Cleaves Phe-Ser-Lys-Phe-Ile-/-Ser-Phe-Asp. No activity benzyloxycarbonyl-Ile-4-nitroanilide, aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin or ovalbumin.

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