3.4.21.B50: DegQ peptidase

This is an abbreviated version!
For detailed information about DegQ peptidase, go to the full flat file.

Reaction

Synonyms

B3234, DEG2, Deg2 protease, DegQ, DegQVh, HhoA peptidase, S01.274, S01.482

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.B50 DegQ peptidase

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Results	in table
5	AA Sequence
1	Application
7	Cloned(Commentary)
2	Crystallization (Commentary)
16	General Information
1	Inhibitors
7	Localization
1	Metals/Ions
7	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
127	Organism
3	pH Optimum
2	pH Range
4	Protein Variants
8	Purification (Commentary)
25	Reference
1	Source Tissue
32	Substrates and Products (Substrate)
9	Subunits
28	Synonyms
3	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Crystallization

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Crystallization on EC 3.4.21.B50 - DegQ peptidase

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sitting drop vapor diffusion method

Legionella fallonii

718323

sitting-drop vapor diffusion technique. The best crystals are obtained when using 100 mM citrate (pH 5.5) and 17.5% polyethylene glycol 400 or 100 mM sodium acetate (pH 4.5), 50 mM CdCl2 and 28% polyethylene glycol 400 as reservoir solutions. Crystal structures of inactive 3-mers and active 12-mers of DegQ reveal molecular details of elements of a conserved allosteric activation cascade that defines distinct protease ON and OFF states

Legionella pneumophila subsp. pneumophila

Q5ZVV9

754420