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3.4.21.B48: kumamolysin

This is an abbreviated version!
For detailed information about kumamolysin, go to the full flat file.

Word Map on EC 3.4.21.B48

Reaction

specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate. Good cleavage of the Phe-I-(p-nitrophenylalanine) bond in synthetic peptides. The enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu =

Synonyms

J-4 serine-carboxyl proteinase, KSCP, kumamolisin, kumamolisin serine-carboxyl proteinase, kumamolisin-ac, kumamolisin-AS, kumamolisin-like proteae, kumamolysin, More, SCPA

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B48 kumamolysin

Turnover Number

Turnover Number on EC 3.4.21.B48 - kumamolysin

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54 - 6.08
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
0.00045 - 395
IQF peptide
-
0.26
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.5
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.46 - 6.08
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
20.3
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
98.3
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.98 - 6.08
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
0.22
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
1.04 - 6.08
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
0.19
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
0.41
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.45
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
3.5 - 19.9
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
32.3
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.13 - 6.08
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
3.3 - 4.62
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
3 - 6
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
0.64 - 6.08
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
16.1
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
13.1
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.08 - 6.08
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
48.5
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.04 - 9.14
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
28.6 - 81
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
3.3 - 4.79
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
7.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu
Bacillus novosp.
-
-
21.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
12.5
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile
Bacillus novosp.
-
-
25.4
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
34.2
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle
Bacillus novosp.
-
-
3.7 - 31.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
15.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr
Bacillus novosp.
-
-
13.1
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
0.04 - 8.78
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
22.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu
Bacillus novosp.
-
-
1.82 - 2.94
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
23.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu
Bacillus novosp.
-
-
10.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu
Bacillus novosp.
-
-
2.6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu
Bacillus novosp.
-
-
3.3 - 5.04
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
3
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.3
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.84 - 6.08
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
176
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu
-
pH 3.5, 60°C
176
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
52.7
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
0.73 - 6.08
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
3.13 - 4.44
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
3.13 - 3.76
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
0.052 - 2.1
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.59 - 6.08
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
16.7
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
18.6
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
2.35
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
71
oxidized insulin B-chain
Bacillus novosp.
-
pH 3.0, 30°C
-
0.27
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.033
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
-
mutant enzyme E78H
additional information
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu