3.4.21.B48: kumamolysin

This is an abbreviated version!
For detailed information about kumamolysin, go to the full flat file.

Word Map on EC 3.4.21.B48

3.4.21.B48

peptidase

pepstatin-insensitive

subtilisin

hole

oxyanion

proteinases

collagenase

pepstatin

merops

diazoacetyl-dl-norleucine

sedolisins

celiac

subtilisin-like

acidophilic

xanthomonas

leu15-tyr16

gluten

alicyclobacillus

biotechnology

Reaction

specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate. Good cleavage of the Phe-I-(p-nitrophenylalanine) bond in synthetic peptides. The enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu =

Synonyms

J-4 serine-carboxyl proteinase, KSCP, kumamolisin, kumamolisin serine-carboxyl proteinase, kumamolisin-ac, kumamolisin-AS, kumamolisin-like proteae, kumamolysin, More, SCPA

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.B48 kumamolysin

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Results	in table
1	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
6	Crystallization (Commentary)
2	Disease/ Diagnostics
4	General Information
1	General Stability
9	Inhibitors
3	Ki Value [mM]
78	KM Value [mM]
2	Localization
14	Molecular Weight [Da]
70	Organism
4	pH Optimum
2	pH Range
2	pI Value
12	Posttranslational Modification
32	Protein Variants
3	Purification (Commentary)
6	Reaction
2	Reaction Type
26	Reference
4	Source Tissue
2	Specific Activity [micromol/min/mg]
94	Substrates and Products (Substrate)
15	Subunits
30	Synonyms
5	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
94	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.4.21.B48 - kumamolysin

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Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

2 entries

Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Arg-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Azocoll + H2O

Alicyclobacillus acidocaldarius

677899

Benzyloxycarbonyl-L-Ala-L-Ala-L-Leu 4-nitroanilide + H2O

2 entries

bovine alpha-globulin + H2O

Alicyclobacillus acidocaldarius

677899

Bovine insulin B-chain + H2O

Alicyclobacillus acidocaldarius

677899

casein + H2O

2 entries

Collagen + H2O

Alicyclobacillus acidocaldarius

677899

haemoglobin + H2O

Alicyclobacillus acidocaldarius

677899

high-powder azure + H2O

Alicyclobacillus acidocaldarius

677899

IQF peptide + H2O

2 entries

Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

2 entries

Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Ala-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Arg-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Asp-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Leu-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ala-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ala-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ala-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ala-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O

Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ala

Bacillus novosp.

651773

Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O

Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Arg

Bacillus novosp.

651773

Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O

Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Asp

Bacillus novosp.

651773

Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

2 entries

Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O

Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ser

Bacillus novosp.

651773

Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ala-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Arg-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Arg-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Asn-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Asp-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Asp-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Gln-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Glu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu

2 entries

Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Asn-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Gln-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ala-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ala

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Arg

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Asp

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ile

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Nle

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Phe

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ser

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Tyr

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Val

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asn-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asp-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Glu-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ile-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Leu-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Lys-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ser-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Val-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-His-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Ile-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Leu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Nle-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu + H2O

Lys-Pro-Ile-Pro-Phe + (4-nitrophenyl)-Arg-Leu

2 entries

Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Pro-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Thr-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ile-Val-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Leu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Lys-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Phe-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ser-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Ser-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Thr-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Pro-Val-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Lys-Ser-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

Bacillus novosp.

651773

Oxidized insulin B-chain + H2O

2 entries

Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O

Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu

2 entries

[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O

[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide

2 entries

additional information

4 entries