3.4.21.B48: kumamolysin
This is an abbreviated version!
For detailed information about kumamolysin, go to the full flat file.
Word Map on EC 3.4.21.B48
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3.4.21.B48
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peptidase
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pepstatin-insensitive
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subtilisin
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hole
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oxyanion
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proteinases
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collagenase
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pepstatin
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merops
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diazoacetyl-dl-norleucine
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sedolisins
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celiac
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subtilisin-like
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acidophilic
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xanthomonas
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leu15-tyr16
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gluten
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alicyclobacillus
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biotechnology
- 3.4.21.B48
- peptidase
-
pepstatin-insensitive
- subtilisin
-
hole
-
oxyanion
- proteinases
- collagenase
- pepstatin
- merops
- diazoacetyl-dl-norleucine
- sedolisins
- celiac
-
subtilisin-like
-
acidophilic
- xanthomonas
-
leu15-tyr16
- gluten
-
alicyclobacillus
- biotechnology
Reaction
specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate. Good cleavage of the Phe-I-(p-nitrophenylalanine) bond in synthetic peptides. The enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu =
Synonyms
J-4 serine-carboxyl proteinase, KSCP, kumamolisin, kumamolisin serine-carboxyl proteinase, kumamolisin-ac, kumamolisin-AS, kumamolisin-like proteae, kumamolysin, More, SCPA
ECTree
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Molecular Weight
Molecular Weight on EC 3.4.21.B48 - kumamolysin
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19000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
38000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
41000
43000
45000
57000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
64000
41000
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
41000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
43000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
45000
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
45000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
64000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE