3.4.21.B43: kallikrein 12
This is an abbreviated version!
For detailed information about kallikrein 12, go to the full flat file.
Word Map on EC 3.4.21.B43
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3.4.21.B43
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kallikreins
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klk8
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atopic
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dermatitis
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kazal-type
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diagnostics
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kallikrein-like
- 3.4.21.B43
- kallikreins
- klk8
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atopic
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dermatitis
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kazal-type
- diagnostics
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kallikrein-like
Reaction
proteolytic cleavage of polypeptides =
Synonyms
hK12, K12, kallikrein 12, kallikrein-related peptidase 12, KLK12, S01.020
ECTree
3.4.21.B43 kallikrein 12Advanced search results
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results (Substrates Products
Substrates Products on EC 3.4.21.B43 - kallikrein 12
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Boc-VPR-7-amido-4-methylcoumarin + H2O
Boc-VPR + 7-amino-4-methylcoumarin
-
-
-
?
CCN1 protein + H2O
?
i.e. (cyr61,ctgf, nov), the main cleavage site is localized in the hinge region between the first and second half of the recombinant protein at Lys88-Gly89, Lys208-Arg209, Arg218-Ile219, Lys291-Lys292, and Lys345-Asn346
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-
?
D-Val-Leu-Lys-thiobenzyl ester + H2O
D-Val-Leu-Lys + thiobenzyl alcohol
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100% activity
-
-
?
high molecular weight kininogen + H2O
?
proteolysis pattern, overview. Release of bradykinin, a fragment containing the C-terminal end of kinins. The kininogenase activity of the enzyme is poor due to the resistance of hydrolysis of the K-R bond on the N-terminal side of the bradykinin sequence in the kininogen
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-
?
platelet-derived growth factor B precursor + H2O
mature platelet-derived growth factor B + ?
tert-butyloxycarbonyl-DPR-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-DPR + 7-amino-4-methylcoumarin
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55% activity compared to D-Val-Leu-Lys-thiobenzyl ester
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-
?
tert-butyloxycarbonyl-FSR-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-FSR + 7-amino-4-methylcoumarin
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13% activity compared to D-Val-Leu-Lys-thiobenzyl ester
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-
?
tert-butyloxycarbonyl-QAR-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-QAR + 7-amino-4-methylcoumarin
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98% activity compared to D-Val-Leu-Lys-thiobenzyl ester
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-
?
tert-butyloxycarbonyl-VPR-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-VPR + 7-amino-4-methylcoumarin
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85% activity compared to D-Val-Leu-Lys-thiobenzyl ester
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-
?
Fibronectin + H2O
?
the enzyme cleaves the human extracellular matrix proteins fibronectin and tenascin, both of which are involved in the regulation of endothelial cell adhesion and migration. KLK12-mediated fibronectin proteolysis antagonizes fibronectin polymerization and fibronectin fibril formation by endothelial cells, leading to an increase in cell migration
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-
?
influenza hemagglutinin precursor + H2O
mature influenza hemagglutinin + ?
the enzyme shows a preference for the H1 and H2 subtypes, the amino acids neighboring the arginine cleavage site affect cleavage efficiency
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-
?
influenza hemagglutinin precursor + H2O
mature influenza hemagglutinin + ?
the enzyme shows a preference for the H1 and H2 subtypes, essentially no cleavage of the H3 subtype, the amino acids neighboring the arginine cleavage site affect cleavage efficiency
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-
?
platelet-derived growth factor B precursor + H2O
mature platelet-derived growth factor B + ?
membrane bound zymogen
soluble mature paracrine factor, soluble mature protein
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?
platelet-derived growth factor B precursor + H2O
mature platelet-derived growth factor B + ?
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membrane bound zymogen
soluble mature protein
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?
polypeptide + H2O
peptides
involved in posttranslational processing of polypeptide precursors
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?
tenascin + H2O
?
the enzyme cleaves the human extracellular matrix proteins fibronectin and tenascin, both of which are involved in the regulation of endothelial cell adhesion and migration
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-
?
additional information
?
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hormonal reglation of enzyme expression, overview
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-
?
additional information
?
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hormonal reglation of enzyme expression, overview
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-
?
additional information
?
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no cleavage is observed for tert-butyloxycarbonyl-QGR-7-amido-4-methylcoumarin, benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin, L-Pro-Phe-Arg-7-amido-4-methylcoumarin, succinyl-AFK-7-amido-4-methylcoumarin, succinyl-AAPF-7-amido-4-methylcoumarin, succinyl-ALPF-7-amido-4-methylcoumarin, succinyl-LLVY-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and S2586
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-
?
additional information
?
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active KLK12 possesses trypsin-like activity, targeting peptide bonds featuring either arginine or lysine at their P1 position
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-
?
additional information
?
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Abz-GPFTDVRAAVY-(3-NO2-Tyr) is not efficiently hydrolyzed
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?
additional information
?
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Abz-GPFTDVRAAVY-(3-NO2-Tyr) is not efficiently hydrolyzed
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-
?
