3.4.21.B39: stratum corneum tryptic enzyme
This is an abbreviated version!
For detailed information about stratum corneum tryptic enzyme, go to the full flat file.
Word Map on EC 3.4.21.B39
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3.4.21.B39
-
klk5
-
keratinocytes
-
desquamation
-
cathelicidin
-
netherton
-
spink5
-
rosacea
-
kazal-type
-
lekti
-
filaggrin
-
lympho-epithelial
-
corneodesmosomes
-
transepidermal
-
lymphopoietin
-
corneodesmosin
-
kazal
-
desmocollin
-
corneocyte
-
medicine
-
diagnostics
- 3.4.21.B39
- klk5
- keratinocytes
-
desquamation
- cathelicidin
- netherton
-
spink5
- rosacea
-
kazal-type
- lekti
- filaggrin
-
lympho-epithelial
-
corneodesmosomes
-
transepidermal
- lymphopoietin
- corneodesmosin
-
kazal
-
desmocollin
-
corneocyte
- medicine
- diagnostics
Reaction
proteolytic cleavage of polypeptides =
Synonyms
GK-5, hK5, HSC tryptic enzyme, human kallikrein 5, human stratum corneum trypsin-like enzyme, human tissue kallikrein 5, K5, kallikrein 5, kallikrein protein 5, kallikrein-5, kallikrein-like protein 2, kallikrein-related peptidase 5, KLK-L2, KLK5, More, S01.017, SCTE, strateum-corneum trypsin-like serine protease, stratum corneum tryptic enzyme, tissue kallikrein, tissue kallikrein 5
ECTree
3.4.21.B39 stratum corneum tryptic enzymeAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.21.B39 - stratum corneum tryptic enzyme
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(Tos-Gly-Pro-Arg)2[R110] + H2O
2 Tos-Gly-Pro-Arg + rhodamin110
fluorogenic subtrate for thrombin
-
-
?
Ala-Thr-Pro-Lys-Ile-Phe-Asn + H2O
Ala-Thr-Pro-Lys + Ile-Phe-Asn
-
moderate cleavage efficiency
-
-
?
Asp-Gly-Asp-Lys-Ile-Ile-Glu + H2O
Asp-Gly-Asp-Lys + Ile-Ile-Glu
-
low cleavage efficiency
-
-
?
Asp-Thr-Arg-Ala-Ile-Gly + H2O
Asp-Thr-Arg + Ala-Ile-Gly
-
moderate cleavage efficiency
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Gln-Ala-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Gln-Arg-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Gln-Gly-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Leu-Arg-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin + H2O
Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Leu-Lys-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin + H2O
Boc-Val-Leu-Lys + 7-amino-4-methyl-coumarin
-
-
-
-
?
Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Val-Pro-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Cbz-L-Pro-Phe-Arg-p-nitroanilide + H2O
Cbz-L-Pro-Phe-Arg + 4-nitroaniline
-
-
-
?
D-Ile-Pro-Arg-p-nitroanilide + H2O
D-Ile-Pro-Arg + p-nitroaniline
-
-
-
?
defensin-1alpha + H2O
?
KLK5 is capable of processing defensin-1alpha and may be important in vaginal host defense
-
-
?
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O
diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
FSR-amido-4-methylcoumarin + H2O
FSR + 7-amino-4-methylcoumarin
fluorogenic trypsin-like peptide substrate
-
-
?
Gln-Glu-Asp-Lys-Val-Leu-Gly + H2O
Gln-Glu-Asp-Lys + Val-Leu-Gly
-
low cleavage efficiency
-
-
?
Gln-Gly-Asp-Lys-Ile-Ile-Asp + H2O
Gln-Gly-Asp-Lys + Ile-Ile-Asp
-
low cleavage efficiency
-
-
?
Glu-Thr-Arg-Ile-Ile-Lys + H2O
Glu-Thr-Arg + Ile-Ile-Lys
-
moderate cleavage efficiency
-
-
?
H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
H-Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
hK3 + H2O
?
-
hK5 can inactivate hK3 by subsequent cleavage after activation
-
-
?
human plasminogen + H2O
human plasmin + ?
generation of a 29 kDa fragment
-
-
?
Ile-Gln-Ser-Arg-Ile-Val-Gly + H2O
Ile-Gln-Ser-Arg + Ile-Val-Gly
-
high cleavage efficiency
-
-
?
Ile-Leu-Ser-Arg-Ile-Val
Ile-Leu-Ser-Arg + Ile-Val
-
optimal substrate sequences for KLK5
-
-
?
Ile-Leu-Ser-Arg-Ile-Val-Gly + H2O
Ile-Leu-Ser-Arg + Ile-Val-Gly
-
high cleavage efficiency
-
-
?
insulin-like growth factor-binding protein 1 + H2O
insulin-like growth factor-binding protein 1a + insulin-like growth factor-binding protein 1b
-
cleavage after Lys137
-
-
?
insulin-like growth factor-binding protein 1 + H2O
insulin-like growth factor-binding protein 4a + insulin-like growth factor-binding protein 4b
-
cleavage after Arg170
-
-
?
insulin-like growth factor-binding protein 2 + H2O
insulin-like growth factor-binding protein 2a + insulin-like growth factor-binding protein 2b
-
cleavage after Arg164
-
-
?
insulin-like growth factor-binding protein 3 + H2O
insulin-like growth factor-binding protein 3a + insulin-like growth factor-binding protein 3b + insulin-like growth factor-binding protein 3c
-
cleavage after Arg158 and Arg206
-
-
?
insulin-like growth factor-binding protein 5 + H2O
insulin-like growth factor-binding protein 5a + insulin-like growth factor-binding protein 5b + insulin-like growth factor-binding protein 5c
-
cleavage after Arg123 and Arg212
-
-
?
kallikrein 7 + H2O
?
-
Gln-Gly-Asp-Lys-/-Ile-Ile. KLK7 is activated at a low rate
-
-
?
L-Ile-Pro-Arg-4-nitroanilide + H2O
L-Ile-Pro-Arg + 4-nitroaniline
-
-
-
?
L-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
pro-hK2 + H2O
hK2 + ?
-
hK5 can activate hK2 through cleavage after Lys137
-
-
?
pro-hK3 + H2O
hK3 + ?
-
hK5 can activate hK3 through cleavage after Arg85 and Lys182
-
-
?
Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
pro-[stratum corneum chymotryptic enzyme] + H2O
stratum corneum chymotryptic enzyme + ?
-
stratum corneum tryptic enzyme activates stratum corneum chymotryptic enzyme through cleavage of the 31 kDa proenzyme resulting in the mature 28 kDa enzyme
-
-
?
Ser-Ser-Ser-Arg-Ile-Ile-Asn + H2O
Ser-Ser-Ser-Arg + Ile-Ile-Asn
-
moderate cleavage efficiency
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin + H2O
Tos-Gly-Pro-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
Tos-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O
Tos-Gly-Pro-Lys + 7-amino-4-methylcoumarin
-
-
-
?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
?
VPR-amido-4-methylcoumarin + H2O
VPR + 7-amino-4-methylcoumarin
fluorogenic trypsin-like peptide substrate
-
-
?
Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin + H2O
Z-Gly-Gly-Arg + 7-amino-4-methyl-coumarin
-
-
-
-
?
polypeptide + H2O
peptides
enzyme is regulated by binding to protease inhibitors
-
?
proform filaggrin + H2O
mature filaggrin + ?
the enzyme and profilaggrin co-localize in the stratum granulosum in human epidermis. The enzyme interacts with its substrates in granular cells
-
-
?
proform filaggrin + H2O
mature filaggrin + ?
the filaggrin linker domain is the site for processing, recombinant human 53-kDa filaggrin protein
41-kDa filaggrin fragment
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
-
best substrate
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
best substrate
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
-
best substrate
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
best substrate
-
-
?
additional information
?
-
no activity with AAPF-amido-4-methylcoumarin
-
-
?
additional information
?
-
enzyme shows trypsin-like but not chymotrypsin-like activity
-
-
?
additional information
?
-
-
enzyme shows trypsin-like but not chymotrypsin-like activity
-
-
?
additional information
?
-
the enzyme is differentially expressed at the mRNA and protein level in diverse malignancies including cancer
-
-
?
additional information
?
-
-
the enzyme is differentially expressed at the mRNA and protein level in diverse malignancies including cancer
-
-
?
additional information
?
-
-
KLK5 plays a central role in skin desquamation as an initiating activating enzyme in proteolytic cascades formed by kallikreins
-
-
?
additional information
?
-
displays a trypsin-like specificity with strong preference for Arg at position P1. The S1 pocket of hK5 is very specific for P1-Arg side chains. Thr, Asn, Gln, and Ile are tolerated at position P1, whereas the residual amino acids, in particular with large aromatic side chains and Lys, are hardly accepted. Ser is slightly preferred over Asn, Thr, and Ala at position P2, whereas among all other residues only Arg and Tyr are rejected. Most amino acids, in particular Met, Phe, and Tyr but not Asp, are accepted in position P3. An unusual preference for Gly followed by Tyr, Val, and Pro is observed at position P4
-
-
?
additional information
?
-
-
KLK5 activates itself efficiently at the cleavage site Ser-Ser-Ser-Arg-/-Ile-Ile. KLK5 is able to induce signaling events via proteinase-activated receptor-2. Strong preference for P1-Arg over Lys (Lys is accepted by the S1 pocket to some extent) and the complete exclusion of chymotrypsin-specific substrates with a Phe or Tyr side chain in P1 position. Preference for Ala and Lys at P2, preference for Gly in P4
-
-
?
additional information
?
-
no activity with peptides CPGRVVGGC, and QVEPKKCPGRVVGGCVAHPHS
-
-
?
additional information
?
-
-
no activity with tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin, succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, casein, laminin, and single-chain tissue-type plasminogen activator
-
-
?
additional information
?
-
unregulated KLK5 directly activates proteinase-activated receptor 2 and induces nuclear factor kappaB-mediated overexpression of TSLP, intercellular adhesion molecule 1, tumor necrosis factor alpha, and IL8
-
-
?
additional information
?
-
-
unregulated KLK5 directly activates proteinase-activated receptor 2 and induces nuclear factor kappaB-mediated overexpression of TSLP, intercellular adhesion molecule 1, tumor necrosis factor alpha, and IL8
-
-
?
