3.4.21.B39: stratum corneum tryptic enzyme

This is an abbreviated version!
For detailed information about stratum corneum tryptic enzyme, go to the full flat file.

Reaction

proteolytic cleavage of polypeptides =

Synonyms

GK-5, hK5, HSC tryptic enzyme, human kallikrein 5, human stratum corneum trypsin-like enzyme, human tissue kallikrein 5, K5, kallikrein 5, kallikrein protein 5, kallikrein-5, kallikrein-like protein 2, kallikrein-related peptidase 5, KLK-L2, KLK5, More, S01.017, SCTE, strateum-corneum trypsin-like serine protease, stratum corneum tryptic enzyme, tissue kallikrein, tissue kallikrein 5

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B39 stratum corneum tryptic enzyme

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Results	in table
7	Activating Compound
3	AA Sequence
6	Application
2	CAS Registry Number
13	Cloned(Commentary)
3	Crystallization (Commentary)
237	Disease/ Diagnostics
6	Expression
11	General Information
8	IC50 Value
51	Inhibitors
1	kcat/KM [mM/s]
17	Ki Value [mM]
20	KM Value [mM]
3	Localization
8	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
43	Organism
6	pH Optimum
1	pH Range
6	Posttranslational Modification
1	Protein Variants
8	Purification (Commentary)
6	Reaction
8	Reaction Type
41	Reference
105	Source Tissue
1	Storage Stability
98	Substrates and Products (Substrate)
2	Subunits
72	Synonyms
3	Temperature Optimum [°C]
18	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.B39 - stratum corneum tryptic enzyme

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

from epidermis

Homo sapiens

Q9Y337

647353

heparin-Sepharose column chromatography and benzamidine-Sepharose column chromatography

Homo sapiens

Q9Y337

681454

Ni2+-chelate column chromatography and Resource Q column chromatography

Mus musculus

-

683017

recombinant as His-tagged enzyme from Escherichia coli BL21(DE3)

Homo sapiens

Q9Y337

647354

recombinant enzyme from CHO-CCL-61 cells and from Pichia pastoris

Homo sapiens

Q9Y337

647355

recombinant from CHO-K1 cells

Homo sapiens

Q9Y337

647353

reversed phase chromatography

Homo sapiens

-

665944

two chromatography steps, 95% purity

Homo sapiens

-

669319