3.4.21.B34: tricorn core protease (archaea)
This is an abbreviated version!
For detailed information about tricorn core protease (archaea), go to the full flat file.
Reaction
Synonyms
TLP, TRI, Tri-ScC77, Tricorn protease homolog 1, trilobed protease
ECTree
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Crystallization
Crystallization on EC 3.4.21.B34 - tricorn core protease (archaea)
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hanging drop vapour diffusion method, with 100 mM sodium acetate pH 4.4, 20 mM MgCl2, 300 mM 1,6-hexanediol and 10% (w/v) PEG 4000
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enzyme in complex with inhibitor: decanoyl-Arg-Val-Arg-Lys-chloromethyl ketone, Asp-Gln-Thr-Gln-Lys-Gln-Tyr-Gln-Glu-Leu-Thr-Phe-Phe-chloromethyl ketone or benzyloxycarbonyl-Phe-PSI[CO-CONH]Arg-Glu-Phe-OH, hanging drop vapour diffusion method
hanging drop vapour diffusion method, crystal structure at 2.0 resolution, recombinant protein
hanging drop vapour diffusion method, forms crystals of octahedral morphology under low-ionic-strength conditions. Crystals belong to space group C2 with unit cell dimensions, a = 307.5 A, b = 163.2 A, c =220.9 A, beta = 105.5° and diffract to 2.2 A resolution