3.4.21.B28: fibroblast activation protein alpha subunit

This is an abbreviated version!
For detailed information about fibroblast activation protein alpha subunit, go to the full flat file.

Reaction

fibroblast activation protein requires substrates with Pro at P1 and Gly or D-amino acids at P2. Met-alpha2-antiplasmin is cleaved N-terminally at Pro3-Leu4 and Pro12-Asn13 into Asn-alpha2-antiplasmin that is rapidly cross-linked to fibrin and protects it from digestion by plasmin =

Synonyms

antiplasmin-cleaving enzyme, APCE, FAP, FAP-alpha, FAP-specific protease, FAPalpha, fibroblast activation factor, fibroblast activation protein, fibroblast activation protein alpha, fibroblast activation protein protease, fibroblast activation protein-alpha, fibroblast activation protein-specific protease, gelatinase, S09.007, seprase, surface expressed protease, type II transmembrane serine protease, Z-Pro-prolinal-insensitive peptidase, ZIP

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B28 fibroblast activation protein alpha subunit

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Results	in table
1	Activating Compound
4	AA Sequence
11	Application
1	CAS Registry Number
15	Cloned(Commentary)
1	Crystallization (Commentary)
9104	Disease/ Diagnostics
1	Expression
15	General Information
40	IC50 Value
122	Inhibitors
1	kcat/KM [mM/s]
51	Ki Value [mM]
93	KM Value [mM]
23	Localization
31	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
193	Organism
7	pH Optimum
2	pI Value
9	Posttranslational Modification
28	Protein Variants
14	Purification (Commentary)
4	Reaction
12	Reaction Type
54	Reference
124	Source Tissue
171	Substrates and Products (Substrate)
15	Subunits
111	Synonyms
3	Temperature Optimum [°C]
80	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.4.21.B28 - fibroblast activation protein alpha subunit

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

7-methoxycoumarin-4-acetic acid-APGSKGDA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-ASGPAGPA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-DKGESGPA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-DRGETGP-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-DRGETGPA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-DSGETGP-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-DSGETGPA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-EPGPPGPA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-ERGETGPA-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-ERGETGPAG-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-ERGETGPAGG-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-ERGETGPSG-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

7-methoxycoumarin-4-acetic acid-VGPAGK-dinitrophenyl + H2O

?

Homo sapiens

-

-

678329

-

-

?

acetyl-D-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

acetyl-D-Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

Q12884

-

692333

-

-

?

acetyl-Gly-Pro-7-amido-4-methylcoumarin + H2O

acetyl-Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O

acetyl-Gly-Pro-7-amino-4-trifluoromethylcoumarin

Homo sapiens

Q12884

-

678272

-

-

?

acetyl-Gly-Pro-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate + H2O

acetyl-Gly-Pro + 9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate

Mus musculus

-

-

678501

-

-

?

acetyl-Gly-Pro-Gly-Pro-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate + H2O

acetyl-Gly-Pro-Gly-Pro + 9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate

Mus musculus

-

most efficient substrate, FAP shows a greater catalytic efficiency for acetyl-Gly-Pro-Gly-Pro-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate than for acetyl-Gly-Pro-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate and TSGP-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate

678501

-

-

?

Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O

Ala-Pro + 7-amino-4-trifluoromethylcoumarin

2 entries

Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O

Ala-Pro-4 + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

651537

-

651537

?

Ala-Pro-7-amido-4-methylcoumarin + H2O

Ala-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

674436, 680739

-

-

?

Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Ala-Pro + 7-amino-4-trifluoromethylcoumarin + H2O

Homo sapiens

-

-

678329

-

-

?

Ala-Pro-Ala-Pro-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate + H2O

Ala-Pro-Ala-Pro + 9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate

Mus musculus

-

-

678501

-

-

?

Ala-Ser-Gly-Pro-Asn-Gln + H2O

Ala-Ser-Gly-Pro + Asn-Gln

Homo sapiens

Q12884

-

692333

-

-

?

Ala-Ser-Gly-Pro-Ser-Ser + H2O

Ala-Ser-Gly-Pro + Ser-Ser

Homo sapiens

Q12884

-

692333

-

-

?

alpha2-antiplasmin + H2O

?

5 entries

alpha2-antiplasmin + H2O

truncated alpha2-antiplasmin + Met

Homo sapiens

-

-

678913

cleaves Met from the N-terminus yielding Asn as the N-terminal amino acid

-

?

Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg + H2O

Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg

Homo sapiens

-

-

678908

-

-

?

Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg + H2O

Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg

Homo sapiens

Q12884

-

692333

-

-

?

Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg + H2O

Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg

Homo sapiens

Q12884

-

692333

-

-

?

Arg-Pro-7-amido-4-methylcoumarin + H2O

Arg-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

preferred substrate

680739

-

-

?

B-type natriuretic peptide + H2O

?

Homo sapiens

Q12884

efficiently hydrolysed and mostly preferred substrate

717591

-

-

?

benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin + H2O

benzyloxycarbonyl-Gly-L-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

718048

-

-

?

benzyloxycarbonyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O

benzyloxycarbonyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin

Homo sapiens

-

-

680739

-

-

?

benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O

benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin

3 entries

biotinyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O

biotinyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin

Homo sapiens

-

-

680739

-

-

?

Boc-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O

Boc-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin

3 entries

Collagen + H2O

?

2 entries

complement C1q tumor necrosis factor-related protein 6 + H2O

?

Mus musculus

-

cleavage at position 26 after a Val-Pro sequence

754737

-

-

?

CXCL-5 + H2O

?

Mus musculus

-

processing of CXCL-5 at position 42, which is after an Ala-Pro sequence

754737

-

-

?

D-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

D-Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

Q12884

-

692333

-

-

?

denatured type I collagen + H2O

?

Homo sapiens

-

-

678329

-

-

?

extracellular matrix protein 1 + H2O

?

Mus musculus

-

cleavage occurrs at position 142 after a Lys-Pro sequence

754737

-

-

?

extracellular matrix protein CN-I + H2O

?

Mus musculus

-

-

754737

-

-

?

extracellular matrix protein CN-III + H2O

?

Mus musculus

-

-

754737

-

-

?

extracellular matrix protein CN-V + H2O

?

Mus musculus

-

-

754737

-

-

?

fibrillin-2 + H2O

?

Mus musculus

-

cleavage occurrs at position 60, in the propeptide domain, after a Gly-Pro sequence

754737

-

-

?

GASGPAGPA + H2O

GASGP + AGPA

Homo sapiens

Q12844

-

731808

-

-

?

Gelatin + H2O

?

10 entries

GEPGPPGPA + H2O

GEP + GPPGP + L-Ala

Homo sapiens

Q12844

-

731808

-

-

?

GFSPFQRED + H2O

?

Homo sapiens

Q12844

low activity

731808

-

-

?

Gln-Pro-7-amido-4-methylcoumarin + H2O

Gln-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Glu-Pro-7-amido-4-methylcoumarin + H2O

Glu-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

glucagon-like peptide-1 + H2O

?

Homo sapiens

Q12884

slow hydrolysis

717591

-

-

?

glucose-dependent insulinotropic peptide + H2O

?

Homo sapiens

Q12884

slow hydrolysis

717591

-

-

?

Gly-Pro-4-trifluoromethylcoumarin-7-amide + H2O

Gly-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

651537

-

651537

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

6 entries

Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Gly-Pro + 7-amino-4-trifluoromethylcoumarin

2 entries

Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Gly-Pro + 7-amino-4-trifluoromethylcoumarin + H2O

Homo sapiens

Q12884

-

678648

-

-

?

GTAGPNQEQE + H2O

GTAGP + NQEQE

Homo sapiens

Q12844

-

731808

-

-

?

GTSGPNQEQE + H2O

GTSGP + NQEQE

Homo sapiens

Q12844

-

731808

-

-

?

Ile-Pro-7-amido-4-methylcoumarin + H2O

Ile-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

most preferred substrate

680739

-

-

?

Ile-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Ile-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

680739

-

-

?

L-Ala-L-Pro-7-amido-4-methylcoumarin + H2O

L-Ala-L-Pro + 7-amino-4-methylcoumarin

Homo sapiens

Q12884

-

717591

-

-

?

L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin

3 entries

L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg + H2O

?

Homo sapiens

Q12884

FRET-peptide

678473

-

-

?

Leu-Pro-7-amido-4-methylcoumarin + H2O

Leu-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Lys-Ala-7-amido-4-trifluoromethylcoumarin + H2O

Lys-Ala + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

680739

-

-

?

Lys-Pro-4-trifluoromethylcoumarin-7-amide + H2O

Lys-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

651968

-

651968

?

Lys-Pro-7-amido-4-methylcoumarin + H2O

Lys-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

lysyl oxidase homolog 2 + H2O

?

Mus musculus

-

cleavage at position 36 after a Tyr-Pro sequence that follows the signal peptide

754737

-

-

?

MEPLGRQLTSGP-7-amido-4-methylcoumarin + H2O

MEPLGRQLTSGP + 7-amino-4-methylcoumarin

Homo sapiens

Q12884

-

691017

-

-

?

MEPLGWQLTSGP-7-amido-4-methylcoumarin + H2O

MEPLGWQLTSGP + 7-amino-4-methylcoumarin

Homo sapiens

Q12884

-

691017

-

-

?

Met-alpha2-antiplasmin + H2O

?

2 entries

Met-Pro-7-amido-4-methylcoumarin + H2O

Met-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

N-benzyloxycarbonyl-Gly-Leu-Phe-His + H2O

?

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O

N-benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Ala + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Ala

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Glu + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Glu

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-His + H2O

N-benzyloxycarbonyl-Gly-Pro + L-His

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Leu + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Leu

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Met + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Met

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Met-His + H2O

N-benzyloxycarbonyl-Gly-Pro + Met-His

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Met-His-Arg-Ser + H2O

N-benzyloxycarbonyl-Gly-Pro + Met-His-Arg-Ser

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Phe + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Phe

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Phe-His + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Phe-L-His

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Phe-His-Arg + H2O

N-benzyloxycarbonyl-Gly-Pro + Phe-His-Arg

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Phe-His-Arg-Ser + H2O

N-benzyloxycarbonyl-Gly-Pro + Phe-His-Arg-Ser

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Ser + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Ser

Bos taurus

-

-

674022

-

-

?

N-benzyloxycarbonyl-Gly-Pro-Tyr + H2O

N-benzyloxycarbonyl-Gly-Pro + L-Tyr

Bos taurus

-

-

674022

-

-

?

N-formyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O

N-formyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin

Homo sapiens

-

-

680739

-

-

?

N-methyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O

N-methyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin

Homo sapiens

-

-

680739

-

-

?

neuropeptide Y + H2O

?

Homo sapiens

Q12884

efficiently hydrolysed and mostly preferred substrate

717591

-

-

?

peptide YY + H2O

?

Homo sapiens

Q12884

efficiently hydrolysed substrate

717591

-

-

?

Phe-Pro-7-amido-4-methylcoumarin + H2O

Phe-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Phe-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Phe-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

680739

-

-

?

pre-digested collagen + H2O

?

Homo sapiens

-

-

707629

-

-

?

precursor Met-alpha2-antiplasmin + H2O

L-Asn-alpha2-antiplasmin + ?

Homo sapiens

-

the enzyme cleaves between Pro12 and Asn13

718048

-

-

?

Pro-Pro-7-amido-4-methylcoumarin + H2O

Pro-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

preferred substrate

680739

-

-

?

RPKPQQFFGLM + H2O

RPKP + L-Gln-L-Gln + FFGLM

Homo sapiens

Q12844

the substance P-derived sequence is cleaved although it does not contain Gly-Pro

731808

-

-

?

Ser-Pro-7-amido-4-methylcoumarin + H2O

Ser-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Substance P + H2O

?

Homo sapiens

Q12884

efficiently hydrolysed substrate

717591

-

-

?

Thr-Ala-Gly-Pro-Asn-Gln + H2O

Thr-Ala-Gly-Pro + Asn-Gln

Homo sapiens

Q12884

-

692333

-

-

?

Thr-Pro-7-amido-4-methylcoumarin + H2O

Thr-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Thr-Ser-Gly-Pro-Asn-Gln + H2O

Thr-Ser-Gly-Pro + Asn-Gln

Homo sapiens

Q12884

-

692333

-

-

?

Thr-Ser-Gly-Pro-Asn-Ser + H2O

Thr-Ser-Gly-Pro + Asn-Ser

Homo sapiens

Q12884

-

692333

-

-

?

Thr-Ser-Gly-Pro-Ser-Gln + H2O

Thr-Ser-Gly-Pro + Ser-Gln

Homo sapiens

Q12884

-

692333

-

-

?

Tic-Pro-7-amido-4-trifluoromethylcoumarin + H2O

?

Homo sapiens

-

-

718048

-

-

?

TSGP-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate + H2O

TSGP + 9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate

Mus musculus

-

-

678501

-

-

?

Type I collagen + H2O

?

3 entries

type III collagen + H2O

?

Mus musculus

-

-

754737

-

-

?

type V collagen + H2O

?

Mus musculus

-

-

754737

-

-

?

Tyr-Pro-7-amido-4-methylcoumarin + H2O

Tyr-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Val-Pro-7-amido-4-methylcoumarin + H2O

Val-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

680739

-

-

?

Z-Gly-L-Pro-7-amido-4-methylcoumarin + H2O

Z-Gly-L-Pro + 7-amino-4-methylcoumarin

Homo sapiens

Q12884

-

717591

-

-

?

additional information

?

-

20 entries

Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O

Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

651968

-

651968

?

Ala-Pro-4-trifluoromethylcoumarin-7-amide + H2O

Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

Q12884

-

653744

-

653744

?

alpha2-antiplasmin + H2O

?

Homo sapiens

-

-

707629

-

-

?

alpha2-antiplasmin + H2O

?

Homo sapiens

Q12844

-

731808

-

-

?

alpha2-antiplasmin + H2O

?

Homo sapiens

Q12884

alpha2-antiplasmin is cleaved N-terminally to Asn-alpha2-antiplasmin that is rapidly cross-linked to fibrin and protects it from digestion by plasmin

691017

-

-

?

alpha2-antiplasmin + H2O

?

Homo sapiens

Q12884

alpha2-antiplasmin is cleaved N-terminally at Pro12-Asn13 to Asn-alpha2-antiplasmin that is rapidly cross-linked to fibrin and protects it from digestion by plasmin

691017

-

-

?

alpha2-antiplasmin + H2O

?

Homo sapiens

Q12884

efficiently hydrolysed substrate

717591

-

-

?

benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O

benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

674436, 678908, 753304

-

-

?

benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O

benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

Q12884

-

678473, 691017

-

-

?

benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O

benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

691017

-

-

?

Boc-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O

Boc-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin

Homo sapiens

Q12844

-

731821

-

-

?

Boc-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O

Boc-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin

Mus musculus

P97321

-

731821

-

-

?

Boc-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O

Boc-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin

Papio sp.

-

-

731821

-

-

?

Collagen + H2O

?

Mus musculus

-

-

755114

-

-

?

Collagen + H2O

?

Mus musculus

-

cleavage of type I collagen by fibroblast activation protein-? enhances class A scavenger receptor mediated macrophage adhesion

755114

-

-

?

Gelatin + H2O

?

Bos taurus

-

-

674022, 678473

-

-

?

Gelatin + H2O

?

Gallus gallus

-

-

678473

-

-

?

Gelatin + H2O

?

Homo sapiens

-

-

651968

-

651968

?

Gelatin + H2O

?

Homo sapiens

-

-

672907, 707629

-

-

?

Gelatin + H2O

?

Homo sapiens

Q12884

-

678473

-

-

?

Gelatin + H2O

?

Homo sapiens

Q12844

-

731808

-

-

?

Gelatin + H2O

?

Homo sapiens

-

seprase-dimer

676269

-

-

?

Gelatin + H2O

?

Homo sapiens

-

gelatin cleavage results in 51 fragments

678329

-

-

?

Gelatin + H2O

?

Mus musculus

P97321

-

678473

-

-

?

Gelatin + H2O

?

Xenopus laevis

Q91651

-

678473

-

-

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

674436, 677928, 680739

-

-

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

Q12884

-

678473, 691017

-

-

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

-

-

691017

-

-

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

Homo sapiens

Q12844

-

731821

-

-

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

Mus musculus

P97321

-

731821

-

-

?

Gly-Pro-7-amido-4-methylcoumarin + H2O

Gly-Pro + 7-amino-4-methylcoumarin

Papio sp.

-

-

731821

-

-

?

Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Gly-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

674436, 680739

-

-

?

Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O

Gly-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

Q12884

-

678272, 678473

-

-

?

L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

-

-

678908

-

-

?

L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Homo sapiens

Q12884

-

678473

-

-

?

L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O

L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin

Mus musculus

P97321

-

678473

-

-

?

Met-alpha2-antiplasmin + H2O

?

Homo sapiens

-

APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4. Conversion of Met-alpha2-antiplasmin by membrane or soluble FAP to the more easily fibrin-incorporable form, Asn-alpha2-antiplasmin, may increase plasmin inhibition within fibrin surrounding certain neoplasms and have an impact on growth and therapeutic susceptibility

678908

-

-

?

Met-alpha2-antiplasmin + H2O

?

Homo sapiens

-

APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4

678908

-

-

?

Type I collagen + H2O

?

Homo sapiens

-

-

651968

-

651968

?

Type I collagen + H2O

?

Homo sapiens

Q12884

-

678272

-

-

?

Type I collagen + H2O

?

Mus musculus

-

-

754737

-

-

?

additional information

?

-

Bos taurus

-

no hydrolysis of Pro-7-amino-4-methylcoumarin, Gly-Pro-7-amino-4-methylcoumarin, Ala-Pro-7-amino-4-methylcoumarin, pGly-His-Pro-7-amino-4-methylcoumarin, N-benzyloxycarbonyl-Pro-Phe, Gly-Gly-Pro-Ala and N-benzyloxycarbonyl-Gly-Leu-Phe-His

674022

-

-

?

additional information

?

-

Homo sapiens

Q12884

glycosylated form has both postprolyl depeptidyl peptidase and postgelatinase activity, nonglycosylated isoform has no detectable gelatinase activity

653744

-

653744

?

additional information

?

-

Homo sapiens

-

glycosylated form has both postprolyl depeptidyl peptidase and postgelatinase activity, nonglycosylated isoform has no detectable gelatinase activity

653744

-

653744

?

additional information

?

-

Homo sapiens

Q12884

may contribute to invasiveness in malignant cancers

650283

-

650283

?

additional information

?

-

Homo sapiens

-

cleaves alpha2-antiplasmin with Arg as the sixth amino acid approximately 8fold faster than alpha2-antiplasmin with Trp at this position

678913

-

-

?

additional information

?

-

Homo sapiens

-

shows very weak activity against acetyl-Ser-Pro-7-amido-4-methylcoumarin, Asp-Pro-7-amido-4-methylcoumarin, His-Pro-7-amido-4-methylcoumarin and Asn-Pro-7-amido-4-methylcoumarin, does not cleave succinyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin, Gly-Ala-7-amido-4-methylcoumarin, acetyl-Ala-Pro-7-amido-4-methylcoumarin, acetyl-Asp-Pro-7-amido-4-methylcoumarin, acetyl-Glu-Pro-7-amido-4-methylcoumarin, acetyl-Phe-Pro-7-amido-4-methylcoumarin, acetyl-His-Pro-7-amido-4-methylcoumarin, acetyl-Ile-Pro-7-amido-4-methylcoumarin, acetyl-Lys-Pro-7-amido-4-methylcoumarin, acetyl-Leu-Pro-7-amido-4-methylcoumarin, acetyl-Met-Pro-7-amido-4-methylcoumarin, acetyl-Asn-Pro-7-amido-4-methylcoumarin, acetyl-Pro-Pro-7-amido-4-methylcoumarin, acetyl-Gln-Pro-7-amido-4-methylcoumarin, acetyl-Arg-Pro-7-amido-4-methylcoumarin, acetyl-Thr-Pro-7-amido-4-methylcoumarin, acetyl-Val-Pro-7-amido-4-methylcoumarin, and acetyl-Tyr-Pro-7-amido-4-methylcoumarin

680739

-

-

?

additional information

?

-

Homo sapiens

Q12884

FAP cleavage of peptide libraries of short amino acid sequences surrounding the scissile bond (-Pro12-Asn13-) indicates that Gly is required at position P2 and Pro is required at position P1. Arg is optimal at position P7. Peptide cleavage rate increases with Arg in position P6. Placing Arg in P4 or P8 reduces cleavage rates dramatically

691017

-

-

?

additional information

?

-

Homo sapiens

-

FAP cleavage of peptide libraries of short amino acid sequences surrounding the scissile bond (-Pro12-Asn13-) indicates that Gly is required at position P2 and Pro is required at position P1. Arg is optimal at position P7. Peptide cleavage rate increases with Arg in position P6. Placing Arg in P4 or P8 reduces cleavage rates dramatically

691017

-

-

?

additional information

?

-

Homo sapiens

Q12884

fibroblast activation protein requires substrates with Pro at P1 and Gly or D-amino acids at P2. Besides glycine, fibroblast activation protein tolerates substrates with D-Ala and D-Ser at P2. Fibroblast activation protein prefers small, uncharged amino acids at P3, but tolerates most amino acids at P4, P1' and P2'

692333

-

-

?

additional information

?

-

Homo sapiens

-

does not cleave native human collagens, fibronectin or laminin

707629

-

-

?

additional information

?

-

Homo sapiens

-

FAP-alpha is an endopeptidase cleaving large protein with Gly(2)-Pro(1)-cleaving specificity

707629

-

-

?

additional information

?

-

Homo sapiens

Q12884

does not hydrolyze succinyl-L-Ala-L-Pro-7-amido-4-methylcoumarin and H-Gly-Pro-7-amido-4-methylcoumarin

717591

-

-

?

additional information

?

-

Homo sapiens

Q12884

the enzyme acts as both a dipeptidyl peptidase and an endopeptidyl peptidase

717591

-

-

?

additional information

?

-

Homo sapiens

Q12844

a post-proline cleaving serine peptidase

731808

-

-

?

additional information

?

-

Homo sapiens

-

a post-proline cleaving serine peptidase

731808

-

-

?

additional information

?

-

Homo sapiens

Q12844

the enzyme exhibits post-proline cleaving dipeptidyl peptidase and endopeptidase activity toward gelatin and alpha2-antiplasmin. Substrate specificity analysis using a internally quenched fluorogenic probes library for screening, overview. The sequence Pro-Tyr-Asp is strongly cleaved by the enzyme, sequence specificity, detailed overview

731808

-

-

?

additional information

?

-

Homo sapiens

-

the enzyme exhibits post-proline cleaving dipeptidyl peptidase and endopeptidase activity toward gelatin and alpha2-antiplasmin. Substrate specificity analysis using a internally quenched fluorogenic probes library for screening, overview. The sequence Pro-Tyr-Asp is strongly cleaved by the enzyme, sequence specificity, detailed overview

731808

-

-

?

additional information

?

-

Homo sapiens

Q12844

the enzyme is a is a prolyl specific serine protease

732455

-

-

?

additional information

?

-

Mus musculus

-

shows no activity with Gly-Pro-Gly-Pro-9-di-3-sulfonylpropylaminobenzo[a]phenoxazonium perchlorate

678501

-

-

?

additional information

?

-

Mus musculus

-

the enzyme has both dipeptidyl aminopeptidase and endopeptidase activities, and can hydrolyze the post-proline bond

754737

-

-

?