3.4.21.B28: fibroblast activation protein alpha subunit

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For detailed information about fibroblast activation protein alpha subunit, go to the full flat file.

Reaction

fibroblast activation protein requires substrates with Pro at P1 and Gly or D-amino acids at P2. Met-alpha2-antiplasmin is cleaved N-terminally at Pro3-Leu4 and Pro12-Asn13 into Asn-alpha2-antiplasmin that is rapidly cross-linked to fibrin and protects it from digestion by plasmin =

Synonyms

antiplasmin-cleaving enzyme, APCE, FAP, FAP-alpha, FAP-specific protease, FAPalpha, fibroblast activation factor, fibroblast activation protein, fibroblast activation protein alpha, fibroblast activation protein protease, fibroblast activation protein-alpha, fibroblast activation protein-specific protease, gelatinase, S09.007, seprase, surface expressed protease, type II transmembrane serine protease, Z-Pro-prolinal-insensitive peptidase, ZIP

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B28 fibroblast activation protein alpha subunit

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Results	in table
1	Activating Compound
4	AA Sequence
11	Application
1	CAS Registry Number
15	Cloned(Commentary)
1	Crystallization (Commentary)
9104	Disease/ Diagnostics
1	Expression
15	General Information
40	IC50 Value
122	Inhibitors
1	kcat/KM [mM/s]
51	Ki Value [mM]
93	KM Value [mM]
23	Localization
31	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
193	Organism
7	pH Optimum
2	pI Value
9	Posttranslational Modification
28	Protein Variants
14	Purification (Commentary)
4	Reaction
12	Reaction Type
54	Reference
124	Source Tissue
171	Substrates and Products (Substrate)
15	Subunits
111	Synonyms
3	Temperature Optimum [°C]
80	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.B28 - fibroblast activation protein alpha subunit

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

ammonium sulfate precipitation, hydrophobic interaction column chromatography, and immunoaffinity column chromatography

Homo sapiens

-

678913

anti-FLAG affinity chromatography

Homo sapiens

Q12884

678272

by column chromatographies, 30197fold to homogeneity

Bos taurus

-

674022

glycosylated form, near homogeneity

Homo sapiens

Q12884

653744

M2-anti-FLAG resin column chromatography

Homo sapiens

-

680739

Ni-NTA resin column chromatography

Homo sapiens

-

678329

partial purification by Sephacryl S-200 gel filtration

Homo sapiens

-

677928

S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography

3 entries

standard protocol

Homo sapiens

-

674436

-

Homo sapiens

-

651537, 678908

-

Homo sapiens

Q12884

678648

S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography

Homo sapiens

Q12884

678473

S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography

Mus musculus

P97321

678473

S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography

Xenopus laevis

Q91651

678473