3.4.21.B28: fibroblast activation protein alpha subunit
This is an abbreviated version!
For detailed information about fibroblast activation protein alpha subunit, go to the full flat file.
Word Map on EC 3.4.21.B28
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3.4.21.B28
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polyposis
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adenomatous
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colorectal
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apc
-
polyneuropathy
-
adenoma
-
polyp
-
amyloid
-
amyloidotic
-
transthyretin
-
hereditary
-
germline
-
colectomy
-
surveillance
-
endoscopic
-
bowel
-
desmoids
-
anastomosis
-
abdominal
-
proctocolectomy
-
duodenal
-
ileal
-
prophylactic
-
pouch
-
extracolonic
-
amyloidogenic
-
ileostomy
-
val30met
-
rectum
-
gardner
-
colonoscopy
-
pouch-anal
-
hnpcc
-
sulindac
-
pancreaticoduodenectomy
-
amyloidoses
-
hamartomatous
-
periampullary
-
lynch
-
ampullary
-
diagnostics
-
mutyh
-
domino
-
polypectomy
-
tafamidis
-
fundic
-
adenoma-carcinoma
-
prealbumin
-
peutz-jeghers
-
medicine
-
fibromatosis
-
nonpolyposis
- 3.4.21.B28
- polyposis
-
adenomatous
- colorectal
- apc
- polyneuropathy
- adenoma
- polyp
-
amyloid
-
amyloidotic
- transthyretin
- hereditary
-
germline
-
colectomy
-
surveillance
-
endoscopic
- bowel
- desmoids
-
anastomosis
- abdominal
-
proctocolectomy
- duodenal
- ileal
-
prophylactic
-
pouch
-
extracolonic
-
amyloidogenic
-
ileostomy
-
val30met
- rectum
-
gardner
-
colonoscopy
-
pouch-anal
-
hnpcc
- sulindac
-
pancreaticoduodenectomy
- amyloidoses
-
hamartomatous
-
periampullary
-
lynch
-
ampullary
- diagnostics
-
mutyh
-
domino
-
polypectomy
-
tafamidis
-
fundic
-
adenoma-carcinoma
-
prealbumin
-
peutz-jeghers
- medicine
- fibromatosis
- nonpolyposis
Reaction
fibroblast activation protein requires substrates with Pro at P1 and Gly or D-amino acids at P2. Met-alpha2-antiplasmin is cleaved N-terminally at Pro3-Leu4 and Pro12-Asn13 into Asn-alpha2-antiplasmin that is rapidly cross-linked to fibrin and protects it from digestion by plasmin =
Synonyms
antiplasmin-cleaving enzyme, APCE, FAP, FAP-alpha, FAP-specific protease, FAPalpha, fibroblast activation factor, fibroblast activation protein, fibroblast activation protein alpha, fibroblast activation protein protease, fibroblast activation protein-alpha, fibroblast activation protein-specific protease, gelatinase, S09.007, seprase, surface expressed protease, type II transmembrane serine protease, Z-Pro-prolinal-insensitive peptidase, ZIP
ECTree
3.4.21.B28 fibroblast activation protein alpha subunitAdvanced search results
results (
results (KM Value
KM Value on EC 3.4.21.B28 - fibroblast activation protein alpha subunit
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.051
7-methoxycoumarin-4-acetic acid-APGSKGDA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0361
7-methoxycoumarin-4-acetic acid-ASGPAGPA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.046
7-methoxycoumarin-4-acetic acid-DKGESGPA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0238
7-methoxycoumarin-4-acetic acid-DRGETGP-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.021
7-methoxycoumarin-4-acetic acid-DRGETGPA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0396
7-methoxycoumarin-4-acetic acid-DSGETGP-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0202
7-methoxycoumarin-4-acetic acid-DSGETGPA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0269
7-methoxycoumarin-4-acetic acid-EPGPPGPA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0567
7-methoxycoumarin-4-acetic acid-ERGETGPA-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.043
7-methoxycoumarin-4-acetic acid-ERGETGPAG-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0371
7-methoxycoumarin-4-acetic acid-ERGETGPAGG-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.0513
7-methoxycoumarin-4-acetic acid-ERGETGPSG-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.052
7-methoxycoumarin-4-acetic acid-VGPAGK-dinitrophenyl
-
in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C
0.026 - 0.029
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg
0.0008
Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg
23°C
0.0006
Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg
23°C
0.106
Ile-Pro-7-amido-4-trifluoromethylcoumarin
-
at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4
0.029
L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg
recombinant enzyme
0.189
Lys-Ala-7-amido-4-trifluoromethylcoumarin
-
at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4
0.245
Phe-Pro-7-amido-4-trifluoromethylcoumarin
-
at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4
0.053
Tic-Pro-7-amido-4-trifluoromethylcoumarin
-
in 25 mM sodium phosphate buffer, pH 7.5, containing 1.0 mM EDTA and 2% (v/v) methanol, at 22°C
0.087
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657N, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.1
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.14
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203D/E204D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.33
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
wild type enzyme, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.42
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657T, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.43
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657V, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.48
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme R123K, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.51
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E204D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.52
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657S, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.57
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203A/E204A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.65
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme R123M, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.67
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme R123A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.69
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E204A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.74
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E204Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.78
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.81
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.84
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme Y656F, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.3
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657F, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.3
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme N704A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.4
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203Q/E204Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
2
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657G, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
2.3
acetyl-Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.244
Ala-Pro-7-amido-4-trifluoromethylcoumarin
-
at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4
0.026
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg
-
pH 7.5, 22°C, APCE
0.029
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg
-
pH 7.5, 22°C, recombinant FAP
0.101
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 22°C, recombinant FAP
0.062
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.091
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.135
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657S, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.2
Gly-Pro-7-amido-4-trifluoromethylcoumarin
Km above 0.2 mM, wild type enzyme
0.248
Gly-Pro-7-amido-4-trifluoromethylcoumarin
-
at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4
0.25
Gly-Pro-7-amido-4-trifluoromethylcoumarin
wild type enzyme, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.37
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme N704A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.38
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme R123A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.41
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657F, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.42
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657T, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.44
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.45
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E204D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.49
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657V, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.55
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203D/E204D, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.67
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme R123M, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.73
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E204A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.75
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E204Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.92
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203A, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.1
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme R123K, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.1
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme Y656F, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.3
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme A657G, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.5
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203Q/E204Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
1.8
Gly-Pro-7-amido-4-trifluoromethylcoumarin
mutant enzyme E203Q, at 23°C in 50 mM Tris (pH 7.4), 100 mM NaCl, and 1 mM EDTA
0.2
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin
Km above 0.2 mM, wild type enzyme
