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3.4.21.B27: proprotein convertase 7

This is an abbreviated version!
For detailed information about proprotein convertase 7, go to the full flat file.

Word Map on EC 3.4.21.B27

Reaction

cleaves proteins typically at sites marked by the consensus Arg-Xaa-(Lys/Arg)-Arg sequence =

Synonyms

furin, LPC, lymphoma PC, PC-7, PC7, PC7A, PC7B, PCSK7, Proprotein convertase, proprotein convertase 7, proprotein convertase PCSK7, proprotein convertase subtilisin/kexin type 7, rPC7, S08.077, SPC7, subtilisin-like proprotein convertase 7

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B27 proprotein convertase 7

Cloned

Cloned on EC 3.4.21.B27 - proprotein convertase 7

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293 and COS-1 cells
expressed in HEK-293 cells, COS-1 cells, and Neuro2A cells
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expressed in Schneider 2 cells
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gene pcsk7, DNA and amino acid sequence determination and analysis, sequence comparison,expression analysis
gene SPC7, DNA and amino acid sequence determination and analysis
isoform PC7B, lacking a 39bp segment compared to PC7A
recombinant enzyme expression of wild-type enzyme mutant enzymes in HuH-7 cells, also expression of the wild-type enzyme in Hep-G2 cells, HEK-293 cells, K-562 cells, CHO cells, and COS-1 cells, functional co-expression with the human transferrin receptor 1 substrate
the distribution of proprotein convertase (PC) activities at the tissue level are monitored by introduction of biosensor CLIP (cell-linked indicator of proteolysis). CLIP v.3 is suitable for ratiometric imaging without interference by FRET, whereas CLIP v.4 can be used as a FRET-based biosensor to quantify PC activities in specific intracellular vesicles, enzyme and CLIP expression in HEK-293T cells. Fusion to the cytosolic tail of PC7 directs CLIP v.4 to compartments that harbor furin activity, but not full-length proprotein convertase 7 (PC7). Overexpressed PC7 cleaves trans-Golgi network/endosomal CLIP v.4 variants
wild-type and truncated form
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