3.4.21.B25: PACE4 proprotein convertase
This is an abbreviated version!
For detailed information about PACE4 proprotein convertase, go to the full flat file.
Word Map on EC 3.4.21.B25
-
3.4.21.B25
-
convertases
-
furin
-
prohormone
-
endoproteases
-
furin-deficient
-
furin-like
-
alpha1-pdx
-
dibasic
-
pro-proteins
-
alpha1-antitrypsin
-
portland
-
endoproteolysis
-
diagnostics
-
kexin
-
kexin-like
-
ski-1
-
cys-rich
-
medicine
-
subtilisin-related
- 3.4.21.B25
-
convertases
- furin
-
prohormone
- endoproteases
-
furin-deficient
-
furin-like
- alpha1-pdx
-
dibasic
-
pro-proteins
- alpha1-antitrypsin
- portland
-
endoproteolysis
- diagnostics
- kexin
-
kexin-like
- ski-1
-
cys-rich
- medicine
-
subtilisin-related
Reaction
releases mature proteins from their proproteins by cleavage of Arg-Xaa-Yaa-Arg-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys =
Synonyms
PACE 4, PACE-4, PACE4, PACE4/SPC4, paired basic amino acid cleaving enzyme 4, paired basic amino acid-cleaving enzyme 4, paired basic amino-acid-cleaving enzyme-4, PC7, proprotein convertase PACE4, S08.075, SPC4, subtilisin-like proprotein convertase, subtilisin-like proprotein convertase 4, subtilisin-like proprotein convertase PACE4, XPACE4
ECTree
3.4.21.B25 PACE4 proprotein convertaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.21.B25 - PACE4 proprotein convertase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
anthrax toxin PA + H2O
?
-
contains the sequence RKKR at the cleavage site
-
?
benzyloxycarbonyl-REKR-7-amido-4-trifluoromethylcoumarin + H2O
?
-
-
-
?
human immunodeficiency virus type 1 Vpr + H2O
?
undergoes proteolytic processing at a very well conserved proprotein convertase cleavage site, R85QRR88-/-, proprotein convertases PC5 and PACE4 can efficiently process extracellular Vpr
-
-
?
pGlu-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O
pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
-
-
?
pro-beta site APP cleaving enzyme + H2O
beta site APP cleaving enzyme + ?
-
i.e. BACE, weak substrate
-
?
proADAMTS-4 + H2O
?
recombinant PACE4 activates proADAMTS-4 by removal of the prodomain
-
-
?
proADAMTS-5 + H2O
?
recombinant PACE4 activates proADAMTS-5 by removal of the prodomain
-
-
?
prosomatostatin + H2O
somatostatin-14 + somatostatin-28
-
enzyme cleaves at a dibasic Arg-Lys site
-
?
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide + H2O
Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
-
-
-
ir
tert-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
tert-butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Vg1 + H2O
?
Vg1 in a TgF beta-related growth factor localized to vegetal blastomers in Xenopus embryos
-
-
?
insulin receptor isoform B zymogen + H2O
mature insulin receptor isoform B + ?
-
-
-
?
insulin receptor isoform B zymogen + H2O
mature insulin receptor isoform B + ?
maturation
-
-
?
preprohepcidin + H2O
?
key role of the convertases furin, PACE4, PC5 and/or PC7 in the generation and secretion of active hepcidin
-
-
?
preprohepcidin + H2O
?
key role of the convertases furin, PACE4, PC5 and/or PC7 in the generation and secretion of active hepcidin. PC7 processes this peptide at the cleavage site of preprohepcidin PMFQRRRRR-/-, resulting in a major 1305 Da N-terminal product. Two minor products with masses of 149 Da and 993 Da are also detected which correspond to the loss of one (PMFQRRRR) or two (PMFQRRR) C-terminal Arg, respectively
-
-
?
proform bone morphogenetic protein 6 + H2O
mature bone morphogenetic protein 6 + ?
-
BMP6, the substrate is exclusively found in hypertrophic chondrocytes
-
-
?
proform bone morphogenetic protein 6 + H2O
mature bone morphogenetic protein 6 + ?
-
BMP6, recombinantly expressed FLAG-tagged human substrate protein. The enzyme recognizes the sequence RTTR(371-374) of human BMP6 and cleaves proBMP6 at its C-terminus
-
-
?
von Willebrand factor precursor + H2O
mature von Willebrand factor + ?
-
-
-
?
von Willebrand factor precursor + H2O
mature von Willebrand factor + ?
-
-
-
?
von Willebrand factor precursor + H2O
mature von Willebrand factor + ?
-
-
?
von Willebrand factor precursor + H2O
mature von Willebrand factor + ?
-
cleavage requires both the P4 arginine and the P2 lysine
-
?
additional information
?
-
-
enzyme cleaves at sites with paired basic amino acid residues
-
?
additional information
?
-
-
PACE4-CS recognizes the motifs X-Ala and X-Pro
-
?
additional information
?
-
-
enzyme binds tightly to heparin and anchors heparan sulfate proteoglycans at the extracellular matrix, role in spatiotemporal regulation of TGFbeta-related factors' biological activity
-
?
additional information
?
-
no activity with insulin receptor isoform A zymogen
-
-
?
additional information
?
-
-
enzyme requires basic residues in the -1, -2 and -4 position of the substrate sequence, can recognize RAAR and, to a much lesser extent, KR and RR
-
?
additional information
?
-
-
role in proteolytic activation of anthrax toxin PA
-
?
additional information
?
-
does not cleave proopiomelanocortin, proglucagon, prosomatostatin and secretogranin in neuroendocrine cells
-
-
?
additional information
?
-
-
the main substrates for PACE4 are membrane type matrix metalloproteinases
-
-
?
additional information
?
-
PACE4 is identified as a proprotein convertase responsible for activation of aggrecanases in osteoarthritic and cytokine-stimulated cartilage
-
-
?
additional information
?
-
PACE4 regulates the submandibular gland development via the activation of some growth/differentiation factors
-
-
?
additional information
?
-
no cleavage of Xnr5, Derrière or ActivinB pro-proteins
-
-
?
additional information
?
-
no cleavage of Xnr5, Derrière or ActivinB pro-proteins
-
-
?
