3.4.21.B12: prostase
This is an abbreviated version!
For detailed information about prostase, go to the full flat file.
Word Map on EC 3.4.21.B12
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3.4.21.B12
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klk4
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kallikreins
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amelogenins
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amelogenesis
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ameloblastin
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enamelysin
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incisor
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imperfecta
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maturation-stage
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amelx
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amelotin
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medicine
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secretory-stage
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hypomineralized
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masp-1
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hypomaturation
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crystallite
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metalloproteinase-20
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analysis
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diagnostics
- 3.4.21.B12
- klk4
- kallikreins
- amelogenins
-
amelogenesis
- ameloblastin
- enamelysin
- incisor
- imperfecta
-
maturation-stage
-
amelx
-
amelotin
- medicine
-
secretory-stage
-
hypomineralized
- masp-1
-
hypomaturation
-
crystallite
-
metalloproteinase-20
- analysis
- diagnostics
Reaction
proteolysis of polypeptides =
Synonyms
EM serine proteinase 1, EMSP1, enamel matrix serine protease 1, HK4, K4, kallikrein 1-related peptidase 4, kallikrein 4, kallikrein-4, kallikrein-4 proteinase, kallikrein-like protein 1, kallikrein-related peptidase, kallikrein-related peptidase 4, kallikrein-related peptidase-4, KLK, KLK-4 proteinase, KLK-L1, KLK4, More, peptidase S01.251, prostase, prostate cancer serine protease, PRSS17, S01.251, serine protease 1, serine protease 17, tissue kallikrein-related peptidase 4
ECTree
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Cloned
Cloned on EC 3.4.21.B12 - prostase
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construction of a chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases, expression in Escherichia coli strain BL21(DE3)
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DNA sequence determination and analysis, chromosome mapping and genomic organization
DNA sequence determination and analysis, chromosome mapping and genomic organization, promotor analysis
expressed in Escherichia coli M15(pREP4) cells and in insect cells (nur hk5)
expression in Escherichia coli strain AD 494 (DE3) with and without the pro-peptide
expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus
gene KLK4, coupling of the coding sequence of mature enzyme to the pro-domain of prostate specific antigen at the 5' end, and V5 and poly-histidine encoding sequences at the 3' end in plasmid pIBm recombinant expression as inactive zymogen in Spodoptera frugiperda insect cell line Sf9
into the BamH/HindIII restriction sites of the pQE30 vector and expressed in Escherichia coli M15[pREP4] cells
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KLK4, 5, 6, and 7 full-length cDNA cloned into vector pRc/RSV. OV-MZ-6 ovarian cancer cells stably co-transfected with plasmids expressing K4-7. These OVKLK4+5+6+7 cells inoculated into the peritoneal cavity of nude mice
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KLK4-pIB/V5-His construct expressed in Sf9 cells. PC-3 cell stimulated with KLK4
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DNA sequence determination and analysis, chromosome mapping and genomic organization
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DNA sequence determination and analysis, chromosome mapping and genomic organization, promotor analysis
Q9JIS2