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3.4.21.B12: prostase

This is an abbreviated version!
For detailed information about prostase, go to the full flat file.

Word Map on EC 3.4.21.B12

Reaction

proteolysis of polypeptides =

Synonyms

EM serine proteinase 1, EMSP1, enamel matrix serine protease 1, HK4, K4, kallikrein 1-related peptidase 4, kallikrein 4, kallikrein-4, kallikrein-4 proteinase, kallikrein-like protein 1, kallikrein-related peptidase, kallikrein-related peptidase 4, kallikrein-related peptidase-4, KLK, KLK-4 proteinase, KLK-L1, KLK4, More, peptidase S01.251, prostase, prostate cancer serine protease, PRSS17, S01.251, serine protease 1, serine protease 17, tissue kallikrein-related peptidase 4

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B12 prostase

Cloned

Cloned on EC 3.4.21.B12 - prostase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
adenovirus-mediated ectopic expression of KLK4 in PC-3 cells and DU145 cells
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construction of a chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases, expression in Escherichia coli strain BL21(DE3)
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DNA and amino acid sequence determination and analysis
DNA sequence determination and analysis, chromosome mapping and genomic organization
DNA sequence determination and analysis, chromosome mapping and genomic organization, promotor analysis
expressed in Escherichia coli
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expressed in Escherichia coli M15(pREP4) cells and in insect cells (nur hk5)
expressed in Rattus norvegicus and Mus musculus
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expression in Escherichia coli strain AD 494 (DE3) with and without the pro-peptide
expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus
expression of inactive zymogen in human 293 kidney cells
gene KLK4, coupling of the coding sequence of mature enzyme to the pro-domain of prostate specific antigen at the 5' end, and V5 and poly-histidine encoding sequences at the 3' end in plasmid pIBm recombinant expression as inactive zymogen in Spodoptera frugiperda insect cell line Sf9
into the BamH/HindIII restriction sites of the pQE30 vector and expressed in Escherichia coli M15[pREP4] cells
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KLK4, 5, 6, and 7 full-length cDNA cloned into vector pRc/RSV. OV-MZ-6 ovarian cancer cells stably co-transfected with plasmids expressing K4-7. These OVKLK4+5+6+7 cells inoculated into the peritoneal cavity of nude mice
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KLK4-pIB/V5-His construct expressed in Sf9 cells. PC-3 cell stimulated with KLK4
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overexpression of KLK4 in PC-3 cells
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