3.4.21.B1: hyaluronan-binding serine protease
This is an abbreviated version!
For detailed information about hyaluronan-binding serine protease, go to the full flat file.
Word Map on EC 3.4.21.B1
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3.4.21.B1
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marburg
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fibrinolysis
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haemostasis
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nonmedullary
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autoactivation
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pro-urokinase
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fnmtc
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medicine
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srgap1
-
analysis
- 3.4.21.B1
-
marburg
-
fibrinolysis
-
haemostasis
-
nonmedullary
-
autoactivation
- pro-urokinase
-
fnmtc
- medicine
-
srgap1
- analysis
Reaction
endopeptidase activity. Cleaves C-terminal site of Lys and Arg =
Synonyms
factor seven activating protease, factor VII activating protease, factor VII-activating protease, FASP, FSAP, gelatinolytic serine proteinase, HABP, HABP2, hyaluronan binding protein 2, hyaluronan-binding protease, hyaluronic acid-binding protease, PHBP, PHBSP, plasma hyaluronan-binding protein, S01.033
ECTree
3.4.21.B1 hyaluronan-binding serine proteaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.4.21.B1 - hyaluronan-binding serine protease
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Ala-Lys-Nle-Arg-7-amido-4-methylcoumarin + H2O
Ala-Lys-Nle-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzoyl-Ile-Glu(gamma-OR)-Gly-Arg-4-nitroanilide + H2O
benzoyl-Ile-Glu(gamma-OR)-Gly-Arg + 4-nitroaniline
i.e. A-2222, weak chromogenic substrate
-
?
CH2SO2-D-cyclohexylalanine-butanoyl-Arg-4-nitroanilide + H2O
CH2SO2-D-cyclohexylalanine-butanoyl-Arg + 4-nitroaniline
i.e. Pefa-5979, chromogenic substrate
-
?
CH2SO2-D-cyclohexylglycine-Gly-Arg-4-nitroanilide
CH2SO2-D-cyclohexylglycine-Gly-Arg + 4-nitroaniline
i.e. Pefa-3107, chromogenic substrate
-
?
CH3-OCO-D-cyclohexylalanine-Gly-Arg-4-nitroanilide + H2O
CH3-OCO-D-cyclohexylalanine-Gly-Arg + 4-nitroaniline
i.e. Pefa-5523, chromogenic substrate
-
?
D-Ala-cyclohexyltyrosine-Lys-4-nitroanilide + H2O
D-Ala-cyclohexyltyrosine-Lys + 4-nitroaniline
i.e. Pefa-5329, weak chromogenic substrate
-
?
D-cyclohexylglycine-Ala-Arg-4-nitroanilide + H2O
D-cyclohexylglycine-Ala-Arg + 4-nitroaniline
i.e. Pefa-5114, good chromogenic substrate
-
?
D-Lys(benzyloxycarbonyl)-Pro-Arg-4-nitroanilide + H2O
D-Lys(benzyloxycarbonyl)-Pro-Arg + 4-nitroaniline
i.e. Pefa-5773, chromogenic substrate
-
?
D-Phe-Pip-Arg-4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
i.e. S-2238, chromogenic substrate
-
?
DL-pyroglutamic acid-cyclohexylglycine-Arg-4-nitroanilide + H2O
DL-pyroglutamic acid-cyclohexylglycine-Arg + 4-nitroaniline
i.e. Pefa-3297, good chromogenic substrate
-
?
fibrinogen + ?
?
-
cleaves the alpha-chain at multiple sites and the beta-chain between lysine53 and lysine54 but not the gamma-chain
-
?
fibroblast growth factor receptor 1 + H2O
?
-
HABP activates the p44/42-dependent MAPK (ERK1/2) signalling cascade independent of the B2-receptor, involving the fibroblast growth factor receptor-1 and basic fibroblast growth factor. This signalling pathway leads to phosphorylation of the kinases Raf, MEK1/2 and ERK1/2
-
-
?
high molecular weight kininogen + H2O
activated cofactor kininogen + bradykinin
-
activates kininogen
-
?
L-pyroglutamic acid-cyclo-hexylglycine-Arg-para-nitroanilide + H2O
?
-
P-3342, fluorogenic substrate
-
?
L-pyroglutamic acid-Pro-Arg-4-nitroanilide + H2O
L-pyroglutamic acid-Pro-Arg + 4-nitroaniline
i.e. S-2366, chromogenic substrate
-
?
L-pyroglutamylglycyl-L-arginine-p-nitroanilide + H2O
L-pyroglutamylglycyl-L-arginine + p-nitroaniline
-
-
-
?
N-tert-butoxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
N-tert-butoxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
synthetic substrate
-
?
single chain urokinase type plasminogen activator + H2O
two chain urokinase type plasminogen activator
-
-
-
-
?
tissue factor pathway inhibitor + H2O
?
-
the enzyme inhibits tissue factor pathway inhibitor, cleavage occurs between kringle domains K1 and K2 (Lys86-Thr87), as well as in the active site of K2 (Arg107-Gly108) and K3 (Arg199-Ala200) domains
-
-
?
urinary plasminogen activator + ?
?
-
converts the inactive single chain urinary plasminogen activator to the active two chain form
-
?
Z-D-Arg-Gly-Arg-4-nitroanilide + H2O
Z-D-Arg-Gly-Arg + 4-nitroaniline
i.e. S-2765, good chromogenic substrate
-
?
basic fibroblast growth factor + H2O
?
-
HABP activates the p44/42-dependent MAPK (ERK1/2) signalling cascade independent of the B2-receptor, involving the fibroblast growth factor receptor-1 and basic fibroblast growth factor. This signalling pathway leads to phosphorylation of the kinases Raf, MEK1/2 and ERK1/2
-
-
?
CTCF + H2O
?
-
essential factor for optimal transcription from the amyloid beta-protein precursor promoter, enzyme cleaves CTCF at three major sites
-
?
CTCF + H2O
?
-
essential factor for optimal transcription from the amyloid beta-protein precursor promoter, enzyme cleaves CTCF at three major sites
-
?
CTCF + H2O
?
-
essential factor for optimal transcription from the amyloid beta-protein precursor promoter, enzyme cleaves CTCF at three major sites
-
?
CTCF + H2O
?
-
essential factor for optimal transcription from the amyloid beta-protein precursor promoter, enzyme cleaves CTCF at three major sites
-
?
Fibrinogen + H2O
?
-
cleaves the alpha-chain at multiple sites and the beta-chain between lysine53 and lysine54 but not the gamma-chain
-
-
?
high molecular weight kininogen + H2O
low molecular weight kininogen + bradykinin
-
-
-
-
?
high molecular weight kininogen + H2O
low molecular weight kininogen + bradykinin
-
HABP triggers Ca2+ release in HUVEC cells via the bradykinin B2 receptor
-
-
?
high molecular weight kininogen profactor + H2O
activated kininogen + bradykinin
-
-
-
-
?
high molecular weight kininogen profactor + H2O
activated kininogen + bradykinin
-
activates kininogen, cleaves between Arg419 and Lys 420
-
?
platelet-derived growth factor-BB + H2O
?
-
the enzyme inhibits platelet-derived growth factor-BB
-
-
?
Protein + H2O
?
-
cleaves C-terminal side of Arg effectively and that of Lys weakly
-
?
additional information
?
-
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enzyme displays pro-urokinase-activating properties as well as factor VII-activating protease activity
-
?
additional information
?
-
-
extracellular activity of HABP affects gene expression level through phosphorylation of two transcription factors, the cAMP responsive element binding protein CREB and the protooncogene c-Myc
-
-
?
additional information
?
-
-
FSAP circulates as single-chain zymogen in plasma, which is autoactivated to an enzymatically active two-chain form
-
-
?
additional information
?
-
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single-chain inactive zymogen is autoactivated to the two-chain active enzyme
-
-
?
additional information
?
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regulation of FSAP activity is dependent on its EGF-3 domain and over-expression of active variants induces cell death
-
-
?
additional information
?
-
-
pro-plasma hyaluronan-binding protein autoproteolytically converts to an active two-chain form with the aid of an effector such as spermidine and heparin. Tannic acid, delphinidin, hamamelitannin, (-)-epicatechin gallate, and 3,5-di-O-caffeoylquinic inhibit spermidine-induced pro-plasma hyaluronan-binding protein autoactivation
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-
?
additional information
?
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substrate Ala-Lys-Nle-Arg-7-amido-4-methylcoumarin is based on a representive FSAP-cleaved sequence obtained from positional scanning substrate combinatorial library method. The results show a predilection for cleavage at a cluster of basic amino acids on the nonprime side. Molecular modelling studies show a preference for Arg over Lys at the P1 site
-
-
?
additional information
?
-
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histone-mediated pro-plasma hyaluronan-binding protein activation in plasma leads to the formations of bradykinin and plasma hyaluronan-binding protein-alpha2-antiplasmin complex as well as histone degradation
-
-
?
