3.4.21.B1: hyaluronan-binding serine protease

This is an abbreviated version!
For detailed information about hyaluronan-binding serine protease, go to the full flat file.

Reaction

endopeptidase activity. Cleaves C-terminal site of Lys and Arg =

Synonyms

factor seven activating protease, factor VII activating protease, factor VII-activating protease, FASP, FSAP, gelatinolytic serine proteinase, HABP, HABP2, hyaluronan binding protein 2, hyaluronan-binding protease, hyaluronic acid-binding protease, PHBP, PHBSP, plasma hyaluronan-binding protein, S01.033

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B1 hyaluronan-binding serine protease

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Results	in table
33	Activating Compound
2	AA Sequence
10	Application
1	CAS Registry Number
5	Cloned(Commentary)
333	Disease/ Diagnostics
2	Expression
11	General Information
52	Inhibitors
1	Ki Value [mM]
2	KM Value [mM]
1	Localization
3	Metals/Ions
29	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
38	Organism
2	pH Optimum
2	pI Value
5	Posttranslational Modification
7	Protein Variants
11	Purification (Commentary)
1	Reaction
26	Reaction Type
38	Reference
1	Renatured (Commentary)
57	Source Tissue
1	Specific Activity [micromol/min/mg]
99	Substrates and Products (Substrate)
8	Subunits
58	Synonyms
1	Temperature Optimum [°C]
1	Temperature Stability [°C]

General Information

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General Information on EC 3.4.21.B1 - hyaluronan-binding serine protease

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

Pagrus major

S6B5D3

the active site residues of a serine proteinase are conserved in the serine protease domain of the enzyme. The enzyme has a similar domain structure and tissue distribution to those of human hyaluronan binding protein 2 (HABP2), putative phylogenetic tree

731684

malfunction

Homo sapiens

-

the variant Marburg I polymorphism results in low enzymatic activity and is associated with an enhanced risk of carotid stenosis and stroke

717169

metabolism

Homo sapiens

Q14520

FSAP is exclusively activated by the positively charged surfaces polyethylenimine and poly-L-lysine, not by the negatively charged glass or self-assembled monolayer with carboxyl group termination or uncharged (Teflon AF) surfaces. Activation is concomitant with coagulation. A contact phase inhibitor diminishes coagulation to background levels for all surfaces except polyethylenimine

752346

physiological function

8 entries

physiological function

Homo sapiens

-

factor VII-activating protease has potential effects on hemostasis

718425

physiological function

Homo sapiens

-

the enzyme plays roles in regulating inflammation, vascular function, fibrosis and atherosclerosis

717319

physiological function

Homo sapiens

-

the enzyme regulates hemostasis- and remodeling-associated processes in the vasculature

717169

physiological function

Pagrus major

S6B5D3

the serine protease is possibly involved in the extracellular matrix turnover. It might be involved in the post-mortem fish muscle softening

731684

physiological function

Homo sapiens

Q14520

FSAP alters fibrin clot properties by fibrinogenolysis. Treatment of human fibrinogen with FSAP releases truncated fibrinogen which shows a delayed thrombin-catalyzed polymerization and forms fibrin clots of reduced turbidity. The clots reveal a less coarse fibrin network with thinner fibers and a smaller pore size. FSAP-treated fibrinogen or plasma exhibits a significantly faster tissue plasminogen activator-driven lysis, which correlates exclusively with cleavage of fibrinogen and not with activation of plasminogen activators

752838

physiological function

Homo sapiens

Q14520

HABP2 overexpression increases LMW-HA-induced urokinase plasminogen activator activation, migration, and extravasation in human lung adenocarcinoma cells. In vivo, overexpression of HABP2 in human lung adenocarcinoma cells increases primary tumor growth rates in nude mice by about 2fold and lung metastasis by about 10fold compared to vector control cells

753699

physiological function

Homo sapiens

Q14520

phorbol ester-mediated neutrophil extracellular trap (NET) formation is marginally stimulated by FSAP. Plasma-derived FSAP as well as exogenous FSAP binds to NET, and FSAP and NETs colocalize in coronary thrombi from patients with acute myocardial infarction. No activation of pro-FSAP by NETs is evident, but after disintegration of NETs with DNase, a robust activation of pro-FSAP, due to release of histones from nucleosomes, is detected. The released histones are in turn degraded by FSAP. Histone cytotoxicity towards endothelial cells is neutralized by FSAP more potently than by activated protein C

755588

physiological function

Homo sapiens

Q14520

upon stimulation of vascular smooth muscle cells (VSMC) and endothelial cells (EC) with FSAP, pathways significantly activated by FSAP include those related to inflammation, apoptosis and cell growth in VSMC and inflammation in EC. The key upregulated genes in VSMC are AREG, PTGS2 and IL6, and in EC these are SELE, VCAM1, and IL8. Secretion of IL6 in VSMC and IL8 in EC is also stimulated by FSAP. Recombinant wild type protease domain of FSAP, but not the Marburg I-isoform, can recapitulate most of these effects. In VSMC, but not EC, gene expression by FSAP is impaired by PAR1 (protease-activated receptor1) receptor antagonists

752625