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3.4.21.98: hepacivirin

This is an abbreviated version!
For detailed information about hepacivirin, go to the full flat file.

Word Map on EC 3.4.21.98

Reaction

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1' =

Synonyms

Cpro-2, Cpro-2 proteinase, Den2 protease, HCV NS3, HCV NS3 protease, HCV NS3/4A, HCV NS3/4A protease, Hepacivirin, hepatitis C virus NS3 serine protease, hepatitis C virus NS3/4A protease, non-structural 3 protein, nonstructural 3 protease, nonstructural protein 3, nonstructural protein 3-4A serine protease, nonstructural protein 3-4Agene, nonstructural protein 3/4A protease, NS2/3 protease, NS2B-NS3 proteinase, NS2B/NS3 protease, NS3, NS3 protease, NS3 protein, NS3 proteinase, NS3 serine protease, NS3 serine proteinase, NS3-4A, NS3-4A protease, NS3-4A serine protease, NS3-4A serine protease complex, NS3-4A serine proteinase, NS3-NS4A protease, NS3-protease, NS3-SP, NS3/4a, NS3/4A protease, NS3/4A protease complex, NS3/4A serine protease, NS34Agene, NS3?4A, NS5A-5B protease, P70, polyprotein-processing proteinase NS3, proteinase NS3, proteinase, polyprotein-processing, NS3, sc-protease, serine protease NS3, serine proteinase NS3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.98 hepacivirin

Cloned

Cloned on EC 3.4.21.98 - hepacivirin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Baculovirus expression system in Sf9 cells
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CF40-gly-NS3pro190 expressed as an N-terminally His-tagged fusion protein in Escherichia coli
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cloning from clinical isolate genotype 4a and recombinant expression of GST-tagged enzyme in Escherichia coli TOP cells
Hepatitis C virus genotype 4a
Escherichia coli JM109 cells containig plasmid pcI.HCVcro that includes the NS5A/NS5B cleavage site transformed with plasmid pHCVNS32-181/421-34 protease. Expression in Escherichia coli XL-1 Blue cells
Escherichia coli JM109 cells containing plasmid pcI.HCVcro that includes the NS5A/NS5B cleavage site transformed with plasmid pHCVNS32-181/421-34 protease. Expression in Escherichia coli XL-1 Blue cells
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) and in Rosetta (DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli cells
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expressed in Huh-7 cells
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expressed in Saccharomyces cerevisiae
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expression in Escherichia coli
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expression in yeast
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expresssed in Escherichia coli
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genotyping, expression of wild-type and mutant variant viruses in Huh7.5 cells
HCV NS3 sequence comparisons and phylogenetic analysis
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minimal precursor (residues 904–1206 of the hepatitis C virus polyprotein, corresponding to the C-terminal cytoplasmic domain of NS2 and the entire protease domain of NS3) introduced into vector pET23a and expressed as an insoluble protein in Escherichia coli strain BL21 (DE3) pLysS
NS3 and NS34A fragments removed from HR’TRIPDU3-NS3 and HR’TRIPDU3(CMV)-NS3/4A, respectively, inserted into pcDNA3.1/Hygro(2)T7
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NS3-4A wild-type and mutants expressed in Huh7 cells. HEK-293 cells transiently transfected with NS3 construct followed by Sendai virus infection
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recombinant expression of wild-type active NS3 and NS3 mutants in LX-2 cells, real-time PCR expression analysis. Protease competent NS3 has a significant fibrogenic impact in LX-2 cells when compared to protease defective NS3 or GFP control plasmids. The expression of miR-122 is downregulated in both versions of the cells transfected with NS3 plasmids irrespective of protease function, impact of protease competent NS3 and protease defective TNS3 on TGF-beta production from LX-2 cells, overview
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recombinant plasmid pMANS34NSH containing MBP-NS3-SP expressed in Escherichia coli JM109 cells. PCI-neo-NS3/4A-SEAP chimeric plasmid expressed in Cos-7 cells
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recombinant single-chain NS3-4A protein containing an N-terminal hexahistidine tag expressed in Escherichia coli BL21(DE3) cells
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residues 1-189 expressed in Escherichia coli as a soluble protein
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residues 1027-1207 of the NS3 protein, expressed in Escherichia coli
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RNA sequence determination
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wild-type or mutants R155K, R155T, R155S, and R155I expressed from the corresponding pBEV10/HCV-3201/NS3181-His6 plasmids in BL21/DE3 Escherichia coli cells. Mutant R155K, fused to a T7 tag at the N terminus and a His6 tag at the C terminus, expressed from pBEV10/HCV-H/NS3181-His6 containing the R155K variation in Escherichia coli BL21 (DE3) cells
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wild-type or mutants V36M, V36L, A156T expressed in Escherichia coli BL21(DE3)
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