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3.4.21.88: Repressor LexA

This is an abbreviated version!
For detailed information about Repressor LexA, go to the full flat file.

Word Map on EC 3.4.21.88

Reaction

Hydrolysis of Ala84-/-Gly bond in repressor LexA =

Synonyms

Cg2114, LexA, LexA protein, LexA repressor, LexA transcriptional repressor, LexA1, lexA_1, MtLexA, SOS regulatory protein dinR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.88 Repressor LexA

Cloned

Cloned on EC 3.4.21.88 - Repressor LexA

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNAs encoding the LexA DNA-binding domain (residues 1-202; X) and the herpes simplex virus regulatory protein VP16 transactivation domain (residues 413-490; V and Vstop) amplified from pEG202
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expression in Escherichia coli
gene lexA, comparative genomics and analysis of the core SOS regulon
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gene lexA, construction of complementing plasmid pLexA from the rcc02361 coding region and flanking sequences are amplified from SB1003 genomic DNA, digested, and cloned into plasmid pCM62
gene lexA, overexpression of His6-tagged enzyme in Escherichia coli strain M15
gene lexA, quantitative enzyme PCR expression analysis
gene lexA, recombinant expression of His-tagged wild-type LexA and mutant enzymes S160A, K197A, and G126D, the isolated C-terminal segment and the N-domain in Escherichia coli strain BL21(DE3) pLysS
LexA repressor is overexpressed using Escherichia coli strain JL652
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overexpression in Escherichia coli
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overexpression in Escherichia coli as wild type, mutants and as truncated proteins
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the amino-terminal DNA binding domain of LexA and full-length LexA ligated into pRSETB vector DNA producing pNLexA which expresses a recombinant 11.4 kDa HIS-LexA polypeptide encoding the N-terminal DNA binding domain. Expression of plasmid constructs in Escherichia coli strains DH5alpha and JM109
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the lexA gene, including its promoter region, cloned into pEJMyc to give pKS04, giving LexA with an in-frame C-terminal Myc tag. Plasmids pKS04, pKS04mut1 (one residue deleted between the possible start codons) and the pEJMyc vector transformed separately into Mycobacterium smegmatis strain mc2155
transformed into Escherichia coli JL2301
When there is no DNA damage, the LexA repressors ensure that both recA and lexA are expressed at low basal levels. Before and after DNA damage the promoter activity of recA is low, yet it exhibits some fluctuations. These fluctuations are due to the low copy number of LexA, which is an auto-repressor. In case that the DNA damage persists, the negative regulation acting on both recA and lexA is eventually removed, and the promoter activity of both genes increases, genetic regulation mechanism, overview
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wild-type strain ATCC 13032 and mutant strain NJ2114, DNA sequence and genetic structure determination and analysis, and gene expression profiling of the lexA. Detection of 46 potential SOS boxes located upstream of differentially expressed transcription units. Binding of a hexahistidyl-tagged LexA protein to 40 double-stranded oligonucleotides containing the potential SOS boxes. LexA binds not only to SOS boxes in the promoter-operator region of upregulated genes, but also to SOS boxes detected upstream of downregulated genes, overview. Genetic organization of the lexA-divS intergenic region deduced from promoter mapping. LexA expression in Escherichia coli strain BL21(DE3)
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