3.4.21.86: Limulus clotting enzyme

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For detailed information about Limulus clotting enzyme, go to the full flat file.

Word Map on EC 3.4.21.86

3.4.21.86

thrombin

horseshoe

crab

tachypleus

tridentatus

amebocyte

coagulogens

proclotting

prothrombinase

coagulin

phenol-water

rennet

molecular biology

analysis

Reaction

Selective cleavage of -Arg18-/- and -Arg47-/- bonds in coagulogen to form coagulin and fragments =

Synonyms

Clotting enzyme, Limulus clotting enzyme

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.86 Limulus clotting enzyme

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Results	in table
2	Activating Compound
8	AA Sequence
2	Application
1	Cloned(Commentary)
1	General Information
2	Localization
4	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
5	Organism
5	Posttranslational Modification
1	Purification (Commentary)
1	Reaction
2	Reaction Type
5	Reference
3	Source Tissue
7	Substrates and Products (Substrate)
2	Subunits
3	Synonyms

Substrates Products

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Substrates Products on EC 3.4.21.86 - Limulus clotting enzyme

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benzyloxycarbonyl-Leu-Gly-Arg-4-aminophenyl ester + H2O

benzyloxycarbonyl-Leu-Gly-Arg + 4-aminophenol

Limulus polyphemus

731131

Coagulogen + H2O

Coagulin + fragments

Limulus sp.

29794

coagulogen + H2O

coagulin + propeptide

2 entries

Limulus amebocyte lysate + H2O

small particles of clotted enzyme

Limulus polyphemus

bacterial endotoxins induce the formation of small particles of clotted enzyme

683070

peptide-4-nitroanilide + H2O

peptide + 4-nitroaniline

Limulus polyphemus

754404

additional information

Limulus polyphemus

the clotting enzyme can be used in the Limulus amebocytelysate (LAL) assay. The kinetic chromogenic LAL assay uses a synthetic peptide-4-nitroanilide substrate that is cleaved by the clotting enzyme, resulting in a product that exhibits a yellow colour. The intensity of the yellow colour or the rate of colour formation correlates with the concentration of LPS in the assayed samples. The coagulation cascade of the LAL system can also be activated via activation of factor G by fungal glucans, although the activation of factor C, which then activates factor B, by LPS is many times more sensitive. Assay method development and evaluation, overview

754404