3.4.21.83: Oligopeptidase B
This is an abbreviated version!
For detailed information about Oligopeptidase B, go to the full flat file.
Word Map on EC 3.4.21.83
-
3.4.21.83
-
gingivalis
-
prolyl
-
leupeptin
-
chymotrypsin-like
-
antipain
-
porphyromonas
-
aprotinin
-
chloromethyl
-
chymostatin
-
tlck
-
tryptase
-
2-macroglobulin
-
benzamidine
-
acrosin
-
phenylmethanesulfonyl
-
proteamaculans
-
bapna
-
drug development
-
medicine
- 3.4.21.83
- gingivalis
-
prolyl
- leupeptin
-
chymotrypsin-like
- antipain
- porphyromonas
- aprotinin
-
chloromethyl
- chymostatin
- tlck
- tryptase
-
2-macroglobulin
- benzamidine
- acrosin
-
phenylmethanesulfonyl
- proteamaculans
-
bapna
- drug development
- medicine
Reaction
Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline =
Synonyms
La_OpB, oligopeptidase B, oligopeptidase B-like, oligopeptidase B2, OP-Tb, OPB, OPB2, OPBTc, Opd B, OpdB, Protease II, Proteinase, Escherichia coli alkaline, II, PSP, Spro_3467, Tb-OP, Tc 120, Tc-OP, trypsin-like protease
ECTree
3.4.21.83 Oligopeptidase BAdvanced search results
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results (KCat KM Value
KCat KM Value on EC 3.4.21.83 - Oligopeptidase B
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2218000
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
pH 8.0, 25°C
547700
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
pH 8.0, 25°C
1
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
21°C, pH not specified in the publication
5.3
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
21°C, pH not specified in the publication
1.1
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
21°C, pH not specified in the publication
3.6
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
21°C, pH not specified in the publication
30
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
120
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
310
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
330
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
4010
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
4040
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
4960
benzoyl-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
3.6
benzoyl-L-Arg-7-amido-4-methylcoumarin
21°C, pH not specified in the publication
129
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37°C
647
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37°C
1480
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37°C
1650
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37°C
251
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
120
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
190
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
880
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
1660
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
2480
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
31
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
5450
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
8910
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
9090
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
22900
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
83
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37°C
217
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37°C
250
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37°C
265
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37°C
750
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37°C
790
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37°C
1030
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37°C
1960
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37°C
2590
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37°C
4960
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37°C
356.8
benzyloxycarbonyl-L-argininyl-L-arginyl-4-nitroanilide
recombinant mutant K655A, pH 8.0, 25°C
2521
benzyloxycarbonyl-L-argininyl-L-arginyl-4-nitroanilide
recombinant mutant K660A, pH 8.0, 25°C
3052.8
benzyloxycarbonyl-L-argininyl-L-arginyl-4-nitroanilide
recombinant mutant D649A, pH 8.0, 25°C
3569.4
benzyloxycarbonyl-L-argininyl-L-arginyl-4-nitroanilide
recombinant mutant D647A, pH 8.0, 25°C
7182.9
benzyloxycarbonyl-L-argininyl-L-arginyl-4-nitroanilide
recombinant wild-type enzyme, pH 8.0, 25°C
16203.6
benzyloxycarbonyl-L-argininyl-L-arginyl-4-nitroanilide
recombinant mutant E125A, pH 8.0, 25°C
191.3
benzyloxycarbonyl-L-lysyl-L-arginyl-4-nitroanilide
recombinant mutant K655A, pH 8.0, 25°C
2697.9
benzyloxycarbonyl-L-lysyl-L-arginyl-4-nitroanilide
recombinant wild-type enzyme, pH 8.0, 25°C
2759.7
benzyloxycarbonyl-L-lysyl-L-arginyl-4-nitroanilide
recombinant mutant K660A, pH 8.0, 25°C
4588.8
benzyloxycarbonyl-L-lysyl-L-arginyl-4-nitroanilide
recombinant mutant D647A, pH 8.0, 25°C
4973.8
benzyloxycarbonyl-L-lysyl-L-arginyl-4-nitroanilide
recombinant mutant E125A, pH 8.0, 25°C
5948.9
benzyloxycarbonyl-L-lysyl-L-arginyl-4-nitroanilide
recombinant mutant D649A, pH 8.0, 25°C
66
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
340
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
2870
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
3370
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
19300
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
1460
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
2310
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
21600
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
33300
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
69400
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
125
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
9.28
Nalpha-benzoyl-DL-argininyl-4-nitroanilide
recombinant mutant K655A, pH 8.0, 25°C
88.56
Nalpha-benzoyl-DL-argininyl-4-nitroanilide
recombinant mutant K660A, pH 8.0, 25°C
171.65
Nalpha-benzoyl-DL-argininyl-4-nitroanilide
recombinant wild-type enzyme, pH 8.0, 25°C
205.7
Nalpha-benzoyl-DL-argininyl-4-nitroanilide
recombinant mutant D647A, pH 8.0, 25°C
602.15
Nalpha-benzoyl-DL-argininyl-4-nitroanilide
recombinant mutant D649A, pH 8.0, 25°C
1385
Nalpha-benzoyl-DL-argininyl-4-nitroanilide
recombinant mutant E125A, pH 8.0, 25°C
2
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
presence of 50 mM Ca2+, pH 8.0, 25°C
5.8
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
pH 8.0, 25°C
485
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25°C
680
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
0.4
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
3
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25°C
