3.4.21.82: Glutamyl endopeptidase II
This is an abbreviated version!
For detailed information about Glutamyl endopeptidase II, go to the full flat file.
Word Map on EC 3.4.21.82
-
3.4.21.82
-
proteinases
-
diisopropyl
-
fluorophosphate
-
casein
-
aureus
-
glu-specific
-
intermedius
-
licheniformis
-
p-nitroanilide
-
alcalase
-
endopeptidases
-
synthesis
- 3.4.21.82
- proteinases
-
diisopropyl
- fluorophosphate
- casein
- aureus
-
glu-specific
- intermedius
- licheniformis
- p-nitroanilide
- alcalase
- endopeptidases
- synthesis
Reaction
Preferential cleavage: -Glu-/- >> -Asp-/-. Preference for Pro or Leu at P2 and Phe at P3. Cleavage of -Glu-/-Asp- and -Glu-/-Pro- bonds is slow =
Synonyms
BL-GSE, GluSGP, Glutamic acid-specific protease, glutamyl endopeptidase 2, glutamyl endopeptidase from Streptomyces griseus, glutamyl endopeptidase II, glutamyl proteinase, rSGPE, SGPE
ECTree
3.4.21.82 Glutamyl endopeptidase IIAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.21.82 - Glutamyl endopeptidase II
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-Aminobenzoyl-Ala-Ala-Glu-Val-Tyr(NO2)-Asp-OH + H2O
2-Aminobenzoyl-Ala-Ala-Glu + Val-Tyr(NO2)-Asp-OH
-
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Gly-Leu-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Ile-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-Ala-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-GLy-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Met-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Nva-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Gly-Leu-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Ile-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Ala-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Gly-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Met-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Nva-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Gly-Leu-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Ile-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Ala-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Gly-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Met-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Nva-NH2
?
-
-
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Gly-Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Ile-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Ala-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Gly-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Met-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Nva-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Gly-Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Ile-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Ala-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Gly-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Met-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Nva-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Gly-Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Ile-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Leu-Ala-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Leu-Gly-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Met-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Nva-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Gly-Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Ile-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Leu-Ala-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Leu-Gly-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Met-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Nva-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Gly-Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Ile-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Leu-Ala-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Leu-Gly-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Leu-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Met-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Nva-NH2
?
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
Benzyloxycarbonyl-Leu-Leu-Glu 2-naphthylamide + H2O
Benzyloxycarbonyl-Leu-Leu-Glu + 2-naphthylamine
-
-
-
-
?
Benzyloxycarbonyl-Phe-Leu-Glu 2-nitroanilide + H2O
Benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline
-
-
-
-
?
Synthetic peptide substrates containing an N-terminal anthraniloyl group and a 3-nitrotyrosine close to the C-terminus + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Ala-Ala-Pro-Asp 4-nitroanilide + H2O
tert-butoxycarbonyl-Ala-Ala-Pro-Asp + 4-nitroaniline
-
slowly
-
-
?
tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O
tert-butoxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
-
-
-
-
?
tert-butoxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O
tert-butoxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Ala-Ala-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Ala-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Ala-Gly-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Gly-Glu + 4-nitroaniline
-
-
-
-
?
tert-butyloxycarbonyl-Ala-Ala-Leu-Glu-4-nitroanilide + H2O
tert-butyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline
-
-
-
-
?
Tert-Butyloxycarbonyl-Ala-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Pro-Glu + 4-nitroaniline
-
-
-
-
?
benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O
?
-
-
-
?
benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O
?
-
-
-
?
insulin + H2O
?
-
hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin
-
?
insulin + H2O
?
-
hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin
-
?
additional information
?
-
-
does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide
-
?
additional information
?
-
-
does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide
-
?
additional information
?
-
-
preferentially hydrolyzes peptide bonds on the carbonyl-terminal side of either glutamic acid or aspartic acid
-
-
?
additional information
?
-
-
preference for aspartic acid at P4, preference for Ala and Val at P3, preference for Pro and Val at P2, proline is disfavored at P3, P1', and P2' and aspartic acid is disfavored at P1'
-
-
?
additional information
?
-
-
the enzyme favors Pro and Leu residues at S2, while S3 subsite prefers Phe over either Ala or Leu
-
-
?
additional information
?
-
-
hydrolyzes Glu-Xaa bonds approximately 100-fold faster than Asp-Xaa bonds
-
-
?
additional information
?
-
-
binding site comprises at least 6 subsites
-
-
?
additional information
?
-
-
pronounced preference for Glu-Xaa bonds versus Asp-Xaa bonds
-
-
?
