3.4.21.81: Streptogrisin B

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For detailed information about Streptogrisin B, go to the full flat file.

Reaction

Hydrolysis of proteins with trypsin-like specificity =

Synonyms

More, Pronase B, protease B, Proteinase B, Proteinase, Streptomyces griseus serine B, Serine proteinase B, SGPB, SprB, Streptogrisin B, Streptomyces griseus peptidase B, Streptomyces griseus protease B, Streptomyces griseus proteinase 1, Streptomyces griseus proteinase B

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.81 Streptogrisin B

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Results	in table
122	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
9	Crystallization (Commentary)
1	General Stability
12	Inhibitors
4	kcat/KM [mM/s]
9	KM Value [mM]
1	Localization
4	Molecular Weight [Da]
2	Organic Solvent Stability
26	Organism
27	PDB ID
6	pH Optimum
1	Posttranslational Modification
6	Protein Variants
5	Purification (Commentary)
1	Reaction
2	Reaction Type
25	Reference
1	Renatured (Commentary)
2	Source Tissue
27	Substrates and Products (Substrate)
3	Subunits
20	Synonyms
1	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
10	Turnover Number [1/s]

Engineering

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Engineering on EC 3.4.21.81 - Streptogrisin B

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

S195A

Streptomyces griseus

-

active-site variant of protease B with peptide-ligation activity

650820

S195A/T213L/F228H

Streptomyces griseus

-

i.e. streptoligase, catalyzes peptide ligation efficiently. Ligation proceeds via an acyl-enzyme intermediate involving H57. Mutant exhibits half-life for unfolding of 16.3 min at 55°C in the absence of stabilizing substrates

664042

S195C

Streptomyces griseus

-

active-site variant of protease B with peptide-ligation activity

650820

S195G

Streptomyces griseus

-

active-site variant of protease B with peptide-ligation activity

650820

S195G/H57N

Streptomyces griseus

-

active-site variant of protease B with peptide-ligation activity

650820

additional information

Streptomyces griseus

-

introduction of site-specific disulfide bonds into the flexible N-terminal loop of natural Kazal-type inhibitors to characterize the thermodynamics of the reactive site peptide bond hydrolysis. Mutant P14C/N39C is a disulfide variant of the ovomucoid third domain from silver pheasant carrying an engineered Cys14–Cys39 bond near the reactive site. The conversion rate of P14C/N39C by streptogrisin B is much faster than that for wild type under any pH condition

718026