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3.4.21.78: granzyme A

This is an abbreviated version!
For detailed information about granzyme A, go to the full flat file.

Word Map on EC 3.4.21.78

Reaction

Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-/-, -Lys-/- >> -Phe-/- in small molecule substrates =

Synonyms

Autocrine thymic lymphoma granzyme-like serine protease, CTL tryptase, CTLA3, Cytotoxic T lymphocyte serine protease, GrA, Gzm A, Gzma, GzmA/K, H factor, Hanukkah factor, HuTPS, T-cell associated protease 1, T-cell derived serine proteinase 1, TSP-1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.78 granzyme A

Cloned

Cloned on EC 3.4.21.78 - granzyme A

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bone marrow cDNA used to amplify Gzma by PCR
-
cDNA subcloned into the yeast vector pPICzalphaA and heterologously expressed in Pichia pastoris
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commercial preparation
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expression in Escherichia coli
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expression in Pichia pastoris
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expression of human recombinant granzyme A zymogen and its activation by the cysteine proteinase cathepsin
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granzyme A expressed in Escherichia coli BL21-DE3 as a proenzyme capable of in vitro activation by enterokinase
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HepG2 cells infected with granzyme A recombinant vaccinia virus
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K562 cells treated with perforin and recombinant human GzmA
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recombinant enzyme expression in Pichia pastoris
recombinant expression of His-tagged enzyme in Escherichia coli