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3.4.21.74: venombin A

This is an abbreviated version!
For detailed information about venombin A, go to the full flat file.

Word Map on EC 3.4.21.74

Reaction

Selective cleavage of Arg-/- bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme =

Synonyms

AaV-SP-II, albofibrase1, alpha fibrinogenase, alpha-Fibrinogenase, Ancrod, Batroxobin, beta-fibrinogenase, Bhalternin, bothrombin, Bothrops atrox serine proteinase, BpirSP-39, coagulant serine protease, Crotalase, Dav-PA, EC 3.4.21.28, EC 3.4.21.29, EC 3.4.21.30, gloshedobin, Habutobin, Lachesis stenophrys venom serine proteinase, metalloproteinase 1, Mutase, acetolactate, Protein C activator, Reptilase, Russell's viper basic coagulant metalloprotease, RVBCMP, RVV protease, snake venom serine protease, TA-2, Thrombin-like enzyme, thrombin-like serine protease, TLE, Vaa serine proteinase homolog 1, VaaSPH-1, VaF1, venom gloshedobin, viper venom serine protease, VLAF, VLBF, VLCII

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.74 venombin A

Purification

Purification on EC 3.4.21.74 - venombin A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography, several forms, separable by electrophoresis and isoelectric focusing
-
Bothrops atrox variety
by gel filtration
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by gel filtration, 3fold
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by hydrophobic, affinity, and ion exchange chromatography
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His-tagged recombinant habutobin fusion protein pET-r-habutobin and Autographa californica nuclear polyhedrosis virus-r-habutobin, purified by bacterial system and baculoviral system. PET-r-habutobin purified on Ni2+-NTA column
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multiple isoforms
-
native enzyme 12.7fold from venom to homogeneity by gel filtration, anion exchange and heparin affinity chromatography
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native enzyme from venom by anion exchange chromatography, gel filtration, and benzamidine affinity chromatography
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native enzyme from venom by cation exchange chromatography, dialysis, and anion exchange chromatography
native enzyme from venom by gel filtration, benzamidine affinity chromatography and reversed-phase gel filtration on a C2/C18 column
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soluble recombinant NusA-fusion gloshedobin from Escherichia coli by hydrophobic interaction chromatograpy, two steps of anion exchange chromatography, and gel filtration