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3.4.21.68: t-Plasminogen activator

This is an abbreviated version!
For detailed information about t-Plasminogen activator, go to the full flat file.

Word Map on EC 3.4.21.68

Reaction

Specific cleavage of Arg-/-Val bond in plasminogen to form plasmin =

Synonyms

Alteplase, BAT-PA, desmoteplase, DSPA beta, DSPA gamma, EC 3.4.21.31, EC 3.4.99.26, human tissue-type plasminogen activator, IV t-PA, IV tissue plasminogen activator, More, plasminogen activator, Plasminogen activator, tissue-type, PLAT, r-tPA, recombinant tissue plasminogen activator, Reteplase, rt-PA, rtPA, sc-tPA, sctPA, single chain enzyme tPA, t-PA, t-PA protein, t-plasminogen activator, tc-tPA, tctPA, Tissue plasminogen activator, tissue type plasminogen activator, tissue-plasminogen activator, Tissue-type plasminogen activator, tissue-type plasmiongen activator, tPA, two chain tPA

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.68 t-Plasminogen activator

Activating Compound

Activating Compound on EC 3.4.21.68 - t-Plasminogen activator

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
-
potentiates mast cell tPA activity and tPA gene expression, abolished in the presence of adenosine deaminase
bradykinin
-
endothelium-dependent vasodilator that stimulates the release of t-PA. Causes a dose-dependent increase in plasma t-PA antigen and activity concentrations in the infused arm of both pregnant and non-pregnant women. The increase in t-PA activity is greater in the nonpregnant women. Bradykinin increases the net release of t-PA antigen and activity in both pregnant women and non-pregnant women. Both net release of active t-PA and plasma t-PA/plasminogen activator inhibitor type 1 ratios are markedly reduced in pregnant women
cyanogen bromide fibrinogen digest (FIBGN)
-
FIBGN is required for the detection of tPA activity chromogenically
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cyanogen bromide-treated fibrinogen (CNBr-Fbg)
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CNBr-Fbg (50 microgram/ml) give a 10-fold enhancement of activation, and addition of 2.86 microg/ml oversulfated chondroitin-6-sulfate or oversulfated fucoidan amplified this to 15-fold. A 25-fold to 35-fold enhancement of activation of glutamic plasminogen by tPA is obtained when 2.86 microgram/ml oversulfated compounds are combined with 16.2 mmol/l lysine and 50 microgram/ml CNBr-Fbg
-
dibutyryl-cAMP
-
slightly increases level of tPA mRNA expression. Concentration-dependently increases tPA activity in both basal and lipopolysaccharide-stimulated rat primary astrocytes. Treatment with 100 micromol increases tPA activity by 757.9% compared with lipopolysaccharide treatment. Differentially modulates MMP-9 and tPA activity through a mechanism related to PKA activation. Co-treatment with lipopolysaccharide and dibutyryl-cAMP does not have an additive effect on tPA mRNA induction
epinephrine
-
intrabrachial infusion of epinephrine (0.1 to 0.3 microg/100 ml per minute) induces greater t-PA release in normotensive subjects as compared with essential hypertensive patients
Fibrin
fibrinogen
-
IBMX
-
increases tPA activity in lipopolysaccharide-stimulated rat primary astrocytes
interleukin-1alpha
-
increases mRNA and protein expression of tPA in a time- and dose-dependent manner. Maximal stimulation at 72 h and 100 U/ml
-
isoproterenol
-
intrabrachial isoproterenol (0.03 mcirog/100 ml per minute) induces a significant increase in t-PA release, an effect blunted by NG-monomethyl-L-arginine
L-lysine
-
addition of 16.2 mmol/l L-lysine give 3fold to 4fold enhancement of activation, which is further enhanced to 5fold to 6fold by addition of 2.86 microgram/ml oversulfated chondroitin-6-sulfate or oversulfated fucoidan
lipopolysaccharide
-
slightly increases level of tPA mRNA expression. Co-treatment with lipopolysaccharide and dibutyryl-cAMP does not have an additive effect on tPA mRNA induction
melanotransferrin
-
-
oversulfated chondroitin-6-sulfate
-
addition of 28.6 microgram/ml gives 2-fold to 4-fold increase in the rate of enhancement of activation of glutamic plasminogen by tPA using 0.05 mol/l Tris buffer (pH 7.35) containing NaCl (0.9%)
-
oversulfated fucoidan
-
addition of 28.6 microgram/ml gives 2-fold to 4-fold increase in the rate of enhancement of activation of glutamic plasminogen by tPA using 0.05 mol/l Tris buffer (pH 7.35) containing NaCl (0.9%)
-
plasminogen activator inhibitor-1
-
dose-dependently facilitates the dissociation of membrane-retained tPA and increases the amounts of tPA-plasminogen activator inhibitor-1 high-molecular-weight complexes in the medium
Polymerized fibrin
-
enhances amidolytic activity of both one-chain tPA forms but not of two-chain tPA
-
rolipram
-
increases tPA activity in lipopolysaccharide-stimulated rat primary astrocytes
statins
-
can induce tPA and inhibits plasminogen activator inhibitor-1
-
additional information
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