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3.4.21.62: Subtilisin

This is an abbreviated version!
For detailed information about Subtilisin, go to the full flat file.

Word Map on EC 3.4.21.62

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides =

Synonyms

AcpII, Ak.1 protease, Alcalase, Alcalase 0.6L, Alcalase 2.5L, ALE1 subtilase, ALK-enzyme, Alkaline mesentericopeptidase, Alkaline protease, alkaline serine protease, ALP1, Alzwiprase, aprE, AprE51, aqualysin, Arp, AsES, Asp v 13, AtSBT1.9, Bacillopeptidase A, Bacillopeptidase B, Bacillus gibsonii alkaline protease, Bacillus subtilis alkaline proteinase Bioprase, BgAP, Bioprase AL 15, Bioprase APL 30, BLS, BPN', BprB, BprV, C1 subtilase, cold active subtilisin-like serine proteinase, Colistinase, EC 3.4.21.14, EC 3.4.4.16, Esperase, Fe protease, Fe prtS8A, Genenase I, intracellular subtilisin protease, ISP, IvaP, Kazusase, Maxatase, mesenteroicopeptidase, More, Nagarse, Opticlean, ORF2, Orientase 10B, P69 subtilase, PBANKA_1106900, PbSOPT, Peptidase, subtilo-, A, PF3D7_0507300, phytophase, PIMMS2, Protease S, Protease VIII, Protease XXVII, Proteinase K, Proteinase, Bacillus subtilis alkaline, Protin A 3L, PSP-3, psychrophilic subtilisin-like protease, S1P subtilase, SAP, SAS-1, SASP, saspase, Savinase, Savinase 16.0L, Savinase 32.0 L EX, Savinase 4.0T, Savinase 8.0L, savinaseTM, SBc, SBL, SDD1 subtilase, senescence-associated subtilisin protease, SES7, SISBT3 subtilase, SOPT, SP 266, Sspa, SSU0757, SUB1, SUB2, subC, subtilase, subtilase subfamily 1 member 9, subtilase-like protease, subtilisin, subtilisin 72, subtilisin A, Subtilisin amylosacchariticus, Subtilisin BL, subtilisin BPN’, subtilisin C., subtilisin Carlsberg, subtilisin DJ-4, Subtilisin DY, Subtilisin E, subtilisin E-S7, Subtilisin GX, subtilisin JB1, subtilisin Karlsberg, Subtilisin Novo, subtilisin Pr1-like protease, subtilisin protease, subtilisin QK, Subtilisin S41, subtilisin S4I, subtilisin S88, Subtilisin Sendai, subtilisin Sph, subtilisin-like ookinete protein, subtilisin-like ookinete protein important for transmission, subtilisin-like protease, subtilisin-like protease AprV2, subtilisin-like serine protease, Subtilisn J, Subtilopeptidase, Superase, thermitase, thermo-active subtilisin-like serine protease, Thermoase, Thermoase PC 10, thermophilic thermitase, thermostable subtilisin, ThSS45, Tk-subtilisin, trans-cinnamoyl-subtilisin, V. cholerae-secreted serine protease, VC_0157, vPR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.62 Subtilisin

Temperature Stability

Temperature Stability on EC 3.4.21.62 - Subtilisin

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
heating the recombinant protease at 100°C results in a total loss of activity
22 - 45
-
presence of Ca2+
30
-
purified recombinant enzyme, 60 min, 70% activity remaining
30 - 40
-
pH 10.5, stable for 2 h
37
-
relatively unstable when incubated at 37°C for 18 h (80% reduction)
45
-
heating the recombinant protease at 45°C results in a 20% reduction of activity
62 - 71
-
increasing the level of glycosylation causes a linearly dependent reduction in structural dynamics, which leads to an increase in thermostability: subtilisin without bound lactose: 64°C, 1 mol lactose bound per 1 mol subtilisin: 62°C, 2.2 mol lactose bound per 1 mol subtilisin: 69°C and 3.5 mol lactose bound per 1 mol subtilisin: 71°C
70 - 105
highly thermostable, heat-treating for 2 h at 70°C activates the protease, half-life at 85°C in presence of DTT is 34 min, in absence of DTT 317 min, at 105°C complete loss of activity is virtually instantaneous in absence of sorbitol, in presence of 90% sorbitol the half-life is 104 min
80
-
subtilisin-like serine protease is stable at 80°C for at least 3. It loses activity at 90°C and 100°C with half-lives of more than 3 h and 100 min, respectively. Subtilisin loses its activity at 100°C with a half-life of 50 min
80 - 90
purified recombinant enzyme, inactivation within 30 min
additional information