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3.4.21.62: Subtilisin

This is an abbreviated version!
For detailed information about Subtilisin, go to the full flat file.

Word Map on EC 3.4.21.62

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides =

Synonyms

AcpII, Ak.1 protease, Alcalase, Alcalase 0.6L, Alcalase 2.5L, ALE1 subtilase, ALK-enzyme, Alkaline mesentericopeptidase, Alkaline protease, alkaline serine protease, ALP1, Alzwiprase, aprE, AprE51, aqualysin, Arp, AsES, Asp v 13, AtSBT1.9, Bacillopeptidase A, Bacillopeptidase B, Bacillus gibsonii alkaline protease, Bacillus subtilis alkaline proteinase Bioprase, BgAP, Bioprase AL 15, Bioprase APL 30, BLS, BPN', BprB, BprV, C1 subtilase, cold active subtilisin-like serine proteinase, Colistinase, EC 3.4.21.14, EC 3.4.4.16, Esperase, Fe protease, Fe prtS8A, Genenase I, intracellular subtilisin protease, ISP, IvaP, Kazusase, Maxatase, mesenteroicopeptidase, More, Nagarse, Opticlean, ORF2, Orientase 10B, P69 subtilase, PBANKA_1106900, PbSOPT, Peptidase, subtilo-, A, PF3D7_0507300, phytophase, PIMMS2, Protease S, Protease VIII, Protease XXVII, Proteinase K, Proteinase, Bacillus subtilis alkaline, Protin A 3L, PSP-3, psychrophilic subtilisin-like protease, S1P subtilase, SAP, SAS-1, SASP, saspase, Savinase, Savinase 16.0L, Savinase 32.0 L EX, Savinase 4.0T, Savinase 8.0L, savinaseTM, SBc, SBL, SDD1 subtilase, senescence-associated subtilisin protease, SES7, SISBT3 subtilase, SOPT, SP 266, Sspa, SSU0757, SUB1, SUB2, subC, subtilase, subtilase subfamily 1 member 9, subtilase-like protease, subtilisin, subtilisin 72, subtilisin A, Subtilisin amylosacchariticus, Subtilisin BL, subtilisin BPN’, subtilisin C., subtilisin Carlsberg, subtilisin DJ-4, Subtilisin DY, Subtilisin E, subtilisin E-S7, Subtilisin GX, subtilisin JB1, subtilisin Karlsberg, Subtilisin Novo, subtilisin Pr1-like protease, subtilisin protease, subtilisin QK, Subtilisin S41, subtilisin S4I, subtilisin S88, Subtilisin Sendai, subtilisin Sph, subtilisin-like ookinete protein, subtilisin-like ookinete protein important for transmission, subtilisin-like protease, subtilisin-like protease AprV2, subtilisin-like serine protease, Subtilisn J, Subtilopeptidase, Superase, thermitase, thermo-active subtilisin-like serine protease, Thermoase, Thermoase PC 10, thermophilic thermitase, thermostable subtilisin, ThSS45, Tk-subtilisin, trans-cinnamoyl-subtilisin, V. cholerae-secreted serine protease, VC_0157, vPR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.62 Subtilisin

Purification

Purification on EC 3.4.21.62 - Subtilisin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography
by affinity purification, more than 95% pure
by ammonium sulfate precipitation followed by gel filtration
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by ammonium sulfate precipitation, to almost homogeneity, 364fold with recovery of 24.9% of the original activity. AcpII-C and AcpII-C-DELTAPA purified 109- and 219fold, with overall yields of 6.9% and 0.36%, respectively
by gel filtration
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by ion-exchange chromatography
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from culture filtrate
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G56S-propeptide and His-tagged G56S-propeptide purified by gel filtration
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G56W-propeptide and G56E-propeptide, S324A-subtilisin purified in denatured form
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mutants purified in a urea-denatured form
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native enzyme from culture filtrate by ultrafiltration, anion exchange and hydrophobic interaction chromatography, to homogeneity
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native extracellular enzyme from culture supernatant by anion exchangc chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and gel filtration, recombinant enzyme from Trichoderma reesei
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native mature enzyme from cell supernatants by anion exchange chromatography to apparent homogeneity
NCIMB10278, gene expressed in Bacillus subtilis DB104/pZS101
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overview: isolation procedure
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pro-S255A purified in the presence of 8 M urea. Refolded by removing urea in the presence of Ca2+. Further purified by gel filtration
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prosubtilisin purified by ammonium sulfate precipitation and gel filtration
purification by single-step affinity chromatography of a crude enzyme preparation (Alcalase 2.0T) from Novo
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purification of pro-subtilisin E wild-type and mutant (Asp32 to Asn) and intramolecular autoprocessing to active subtilisin E in vitro
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purified to 23.5fold by a combination of cation and anion exchange chromatography, ethanol precipitation followed by reverse-phase HPLC
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recombinant enzyme
recombinant enzyme from Escherichia coli to homogeneity
recombinant extracellular enzyme 7.8fold by ammonium sulfate fractionation, N-carbobenzoxy-D-phenylalanyl-triethylenetetramine-Sepharose (z-D-Phe-TETA) affinity chromatography, hydrophobic interaction chromatography, and anion exchange chrmatography, all in presence of 10 mM Ca2+
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant mature enzyme AtSBT1.9 fused to maltose-binding protein from Escherichia coli BL21(DE3) by amylose affinity chromatography
recombinant wild-type and mutant enzymes from Bacillus subtilis strain DB104 by dialysis and anion and cation exchange chromatography
GN111900.1
recombinant wild-type and mutant enzymes from Bacillus subtilis strain WB600 culture supernatant by anion and cation exchange chromatography and ultrafilatration
S324A-subtilisin and propeptide, by gel filtration
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subtilisin BPN' and recombinant variants
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subtilisin-like serine protease and Pro-S359A, at 4°C, by sonication, centrifugation, ammonium sulfate precipitation and gel filtration
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to homogeneity by ion exchange chromatography and gel filtration. Alkaline proteolytic activity enriched 1480fold with a yield of 27%
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using a HiTrap Q HP column
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using ion-exchange chromatography, ultrafiltration and gel filtration
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using Ni-NTA chromatography
wild-type and mutant enzymes
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