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3.4.21.62: Subtilisin

This is an abbreviated version!
For detailed information about Subtilisin, go to the full flat file.

Word Map on EC 3.4.21.62

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides =

Synonyms

AcpII, Ak.1 protease, Alcalase, Alcalase 0.6L, Alcalase 2.5L, ALE1 subtilase, ALK-enzyme, Alkaline mesentericopeptidase, Alkaline protease, alkaline serine protease, ALP1, Alzwiprase, aprE, AprE51, aqualysin, Arp, AsES, Asp v 13, AtSBT1.9, Bacillopeptidase A, Bacillopeptidase B, Bacillus gibsonii alkaline protease, Bacillus subtilis alkaline proteinase Bioprase, BgAP, Bioprase AL 15, Bioprase APL 30, BLS, BPN', BprB, BprV, C1 subtilase, cold active subtilisin-like serine proteinase, Colistinase, EC 3.4.21.14, EC 3.4.4.16, Esperase, Fe protease, Fe prtS8A, Genenase I, intracellular subtilisin protease, ISP, IvaP, Kazusase, Maxatase, mesenteroicopeptidase, More, Nagarse, Opticlean, ORF2, Orientase 10B, P69 subtilase, PBANKA_1106900, PbSOPT, Peptidase, subtilo-, A, PF3D7_0507300, phytophase, PIMMS2, Protease S, Protease VIII, Protease XXVII, Proteinase K, Proteinase, Bacillus subtilis alkaline, Protin A 3L, PSP-3, psychrophilic subtilisin-like protease, S1P subtilase, SAP, SAS-1, SASP, saspase, Savinase, Savinase 16.0L, Savinase 32.0 L EX, Savinase 4.0T, Savinase 8.0L, savinaseTM, SBc, SBL, SDD1 subtilase, senescence-associated subtilisin protease, SES7, SISBT3 subtilase, SOPT, SP 266, Sspa, SSU0757, SUB1, SUB2, subC, subtilase, subtilase subfamily 1 member 9, subtilase-like protease, subtilisin, subtilisin 72, subtilisin A, Subtilisin amylosacchariticus, Subtilisin BL, subtilisin BPN’, subtilisin C., subtilisin Carlsberg, subtilisin DJ-4, Subtilisin DY, Subtilisin E, subtilisin E-S7, Subtilisin GX, subtilisin JB1, subtilisin Karlsberg, Subtilisin Novo, subtilisin Pr1-like protease, subtilisin protease, subtilisin QK, Subtilisin S41, subtilisin S4I, subtilisin S88, Subtilisin Sendai, subtilisin Sph, subtilisin-like ookinete protein, subtilisin-like ookinete protein important for transmission, subtilisin-like protease, subtilisin-like protease AprV2, subtilisin-like serine protease, Subtilisn J, Subtilopeptidase, Superase, thermitase, thermo-active subtilisin-like serine protease, Thermoase, Thermoase PC 10, thermophilic thermitase, thermostable subtilisin, ThSS45, Tk-subtilisin, trans-cinnamoyl-subtilisin, V. cholerae-secreted serine protease, VC_0157, vPR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.62 Subtilisin

Organic Solvent Stability

Organic Solvent Stability on EC 3.4.21.62 - Subtilisin

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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-methyl-2-propanol
-
effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 56.9% at 85% 2-methyl-2-propanol
acetonitrile
chloroform
-
effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 51.3% at 85% chloroform
dimethylformamide
-
effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 33.55% at 85% dimethylformamide
dioxane
-
in 1,4-dioxane, flexibility of subtilisin Carlsberg decreases during prolonged storage: incubation of lyophilized enzyme for a maximum of 96 h (4 days) in 1,4-dioxane, a solvent in which this enzyme is most active and enantioselective, shows a decrease in the rate of exchange of both fast and slow exchanging protons
Ethanol
-
effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 49.4% at 85% ethanol
Ethyl acetate
-
effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 43.7% at 85% ethyl acetate
Methanol