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3.4.21.62: Subtilisin

This is an abbreviated version!
For detailed information about Subtilisin, go to the full flat file.

Word Map on EC 3.4.21.62

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides =

Synonyms

AcpII, Ak.1 protease, Alcalase, Alcalase 0.6L, Alcalase 2.5L, ALE1 subtilase, ALK-enzyme, Alkaline mesentericopeptidase, Alkaline protease, alkaline serine protease, ALP1, Alzwiprase, aprE, AprE51, aqualysin, Arp, AsES, Asp v 13, AtSBT1.9, Bacillopeptidase A, Bacillopeptidase B, Bacillus gibsonii alkaline protease, Bacillus subtilis alkaline proteinase Bioprase, BgAP, Bioprase AL 15, Bioprase APL 30, BLS, BPN', BprB, BprV, C1 subtilase, cold active subtilisin-like serine proteinase, Colistinase, EC 3.4.21.14, EC 3.4.4.16, Esperase, Fe protease, Fe prtS8A, Genenase I, intracellular subtilisin protease, ISP, IvaP, Kazusase, Maxatase, mesenteroicopeptidase, More, Nagarse, Opticlean, ORF2, Orientase 10B, P69 subtilase, PBANKA_1106900, PbSOPT, Peptidase, subtilo-, A, PF3D7_0507300, phytophase, PIMMS2, Protease S, Protease VIII, Protease XXVII, Proteinase K, Proteinase, Bacillus subtilis alkaline, Protin A 3L, PSP-3, psychrophilic subtilisin-like protease, S1P subtilase, SAP, SAS-1, SASP, saspase, Savinase, Savinase 16.0L, Savinase 32.0 L EX, Savinase 4.0T, Savinase 8.0L, savinaseTM, SBc, SBL, SDD1 subtilase, senescence-associated subtilisin protease, SES7, SISBT3 subtilase, SOPT, SP 266, Sspa, SSU0757, SUB1, SUB2, subC, subtilase, subtilase subfamily 1 member 9, subtilase-like protease, subtilisin, subtilisin 72, subtilisin A, Subtilisin amylosacchariticus, Subtilisin BL, subtilisin BPN’, subtilisin C., subtilisin Carlsberg, subtilisin DJ-4, Subtilisin DY, Subtilisin E, subtilisin E-S7, Subtilisin GX, subtilisin JB1, subtilisin Karlsberg, Subtilisin Novo, subtilisin Pr1-like protease, subtilisin protease, subtilisin QK, Subtilisin S41, subtilisin S4I, subtilisin S88, Subtilisin Sendai, subtilisin Sph, subtilisin-like ookinete protein, subtilisin-like ookinete protein important for transmission, subtilisin-like protease, subtilisin-like protease AprV2, subtilisin-like serine protease, Subtilisn J, Subtilopeptidase, Superase, thermitase, thermo-active subtilisin-like serine protease, Thermoase, Thermoase PC 10, thermophilic thermitase, thermostable subtilisin, ThSS45, Tk-subtilisin, trans-cinnamoyl-subtilisin, V. cholerae-secreted serine protease, VC_0157, vPR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.62 Subtilisin

Molecular Weight

Molecular Weight on EC 3.4.21.62 - Subtilisin

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
101000
calculated fromc DNA
16900
-
SDS-PAGE
169600
-
sequence analysis
170000
-
SDS-PAGE
25000
-
x * 25000, Bacillus sp., SDS-PAGE
26500
-
x * 26500, SDS-PAGE
27000
27230
-
subtilisin Sendai, amino acid sequence
27280
-
subtilisin Carlsberg, amino acid sequence
27290
-
subtilisin Novo, determination from amino acid composition
27400
-
subtilisin Carlsberg, sedimentation equilibrium measurement
27500
27530
-
Bacillus sp., subtilisin BPN', determination from amino acid composition
27636
-
x * 31000, SDS-PAGE, x * 27636, mass spectrometry
27670
-
Bacillus sp., subtilisin amylosacchariticus, amino acid sequence
27800
calculated from the sequence
28000
29000
29140
-
x * 29000, native enzyme, SDS-PAGE, x * 29141, sequence calculation, x * 29140, recombinant enzyme, mass spectrometry
29141
-
x * 29000, native enzyme, SDS-PAGE, x * 29141, sequence calculation, x * 29140, recombinant enzyme, mass spectrometry
31000
31550
mass spectroscopy, processed form
34000
-
x * 34000, SDS-PAGE
34782
MALDI-TOF, 2 * 34782 Da
37000
-
sequence analysis or mass spectrometry
41000
-
SDS-PAGE
41300
calculated online with ProtParam tools
42000
44000
-
Pro-subtilisin-like serine protease derivative with N- and/or C-terminal truncation, gel filtration
44200
-
subtilisin-like serine protease, sequence analysis
44900
x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE
45000
-
pro-S255A, gel filtration
55000
62000
purified prosubtilisin JB1, Western blotting analysis
65000
68630
-
Pro-subtilisin-like serine protease, sequence analysis
69000
x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE
74500
-
MALDI-TOF
80000
82000
Western blot, mature form
84000
-
SDS-PAGE
additional information