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0.85
acetyl-L-Phe
-
30°C, pH 8.0
0.22
acetyl-L-Phe ethyl ester
-
30°C, pH 8.0
4
acetyl-L-Tyr
-
30°C, pH 8.0
0.37
acetyl-L-Tyr ethyl ester
-
30°C, pH 8.0
7 - 10
benzoyl-Arg ethyl ester
2.1
benzoyl-L-Arg ethyl ester
-
30°C, pH 8.0
8.3
benzyloxycarbonyl-Gly-4-nitroanilide
-
pH 7.5, 20°C, in presence and absence of DTT
-
0.0054
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide
-
-
0.059
casein
-
pH 10, 45°C, Vmax: 0.336 mg/min
0.13 - 0.25
Glp-Ala-Ala-Leu-p-nitroanilide
0.98 - 1.99
L-Ala-L-Ala-L-Pro-L-Ala
1.15 - 1.93
L-Ala-L-Ala-L-Pro-L-Phe
0.039 - 0.053
L-Ala-L-Ala-L-Val-L-Ala
0.223 - 0.24
L-Phe-L-Ala-L-Ala-L-Phe
0.62 - 0.75
L-Tyr-L-Val-L-Ala-L-Asp
0.15 - 0.22
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
120
N-Acetyl-Ala methyl ester
-
subtilisin BPN'
66
N-Acetyl-Leu methyl ester
-
subtilisin BPN'
91
N-Acetyl-Lys methyl ester
-
subtilisin BPN'
17
N-acetyl-Phe ethyl ester
-
subtilisin BPN'
60
N-Acetyl-Phe methyl ester
-
subtilisin Novo
24
N-Acetyl-Trp ethyl ester
-
subtilisin BPN'
50
N-Acetyl-Trp methyl ester
-
subtilisin Carlsberg
22 - 70
N-Acetyl-Tyr ethyl ester
90
N-Acetyl-Tyr methyl ester
-
subtilisin Novo, N-acetyl-Tyr ethyl ester, subtilisin Carlsberg, N-acetyl-Trp methyl ester, subtilisin Novo
3.13 - 4.28
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
0.15 - 0.35
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide
0.115 - 0.59
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
0.11 - 7.9
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.000655
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
37°C, pH 10.5
130 - 319
sec-phenethyl alcohol
0.28 - 2.25
Suc-Ala-Ala-Ala-p-nitroanilide
4 - 17
Suc-Ala-Ala-p-nitroanilide
0.33 - 0.92
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
0.68 - 3.37
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
0.0027 - 0.43
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
0.8 - 2
succinyl-AAPA-p-nitroanilide
1.9 - 4.4
succinyl-AAPE-p-nitroanilide
0.39 - 2.46
succinyl-AAPF-p-nitroanilide
3.4 - 8.2
succinyl-AAPR-p-nitroanilide
1.2 - 1.5
Succinyl-Ala-Ala-Ala 4-nitroanilide
0.13
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
0.07 - 1
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
0.36
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
mutant Y217L, pH 8.3, 25°C
0.45
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
mutant Y217L, pH 8.3, 25°C
0.33
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
mutant Y217L, pH 8.3, 25°C
30 - 40
toluenesulfonyl-Arg methyl ester
0.09
Urea-denatured hemoglobin
-
30°C, pH 8.0
-
0.14 - 4
Z-Ala-Ala-Leu-p-nitroanilide
additional information
additional information
-
7
benzoyl-Arg ethyl ester
-
subtilisin Novo, subtilisin Carlsberg
10
benzoyl-Arg ethyl ester
-
subtilisn BPN'
0.13
Glp-Ala-Ala-Leu-p-nitroanilide
-
in 2% DMSO
0.18
Glp-Ala-Ala-Leu-p-nitroanilide
-
in 2% DMF
0.25
Glp-Ala-Ala-Leu-p-nitroanilide
-
in 2% DMF
0.98
L-Ala-L-Ala-L-Pro-L-Ala
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
1.99
L-Ala-L-Ala-L-Pro-L-Ala
-
wild-type, pH 9.0, 24°C
1.15
L-Ala-L-Ala-L-Pro-L-Phe
-
wild-type, pH 9.0, 24°C
1.93
L-Ala-L-Ala-L-Pro-L-Phe
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
0.039
L-Ala-L-Ala-L-Val-L-Ala
-
wild-type, pH 9.0, 24°C
0.053
L-Ala-L-Ala-L-Val-L-Ala
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
0.223
L-Phe-L-Ala-L-Ala-L-Phe
-
wild-type, pH 9.0, 24°C
0.24
L-Phe-L-Ala-L-Ala-L-Phe
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
0.62
L-Tyr-L-Val-L-Ala-L-Asp
-
wild-type, pH 9.0, 24°C
0.75
L-Tyr-L-Val-L-Ala-L-Asp
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
0.15
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
-
0.15
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, subtilisin BPN'
0.15
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
subtilisin DY
0.22
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
subtilisin Carlsberg
22
N-Acetyl-Tyr ethyl ester
-
subtilisin BPN'
70
N-Acetyl-Tyr ethyl ester
-
subtilisin Novo, N-acetyl-Tyr methyl ester, subtilisin Carlsberg
3.13
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C
3.74
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, mutant D182G
3.84
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, wild-type
4.02
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, mutant D180G/D182G
4.28
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, mutant D180G
0.15
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.4, 37°C, mutant S101W/G169A/V192A
0.35
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.4, 37°C, wild-type
0.115
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 5°C, pH 8.6
0.133
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 25°C, pH 9.4
0.15
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/L216W, pH 7.5, 30°C
0.178
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 25°C, pH 8.6
0.198
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 25°C, pH 7.4
0.2
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/G165Y/L216W, pH 7.5, 30°C
0.227
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant N212G
0.227
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 45°C, pH 8.6
0.233
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant wild-type enzyme
0.278
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210G
0.279
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant wmutant T211G
0.29
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210G/T211G
0.301
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210G/T211G/N212G
0.325
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210A
0.42
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/G165L/L216W, pH 7.5, 30°C
0.54
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/G165I/L216W, pH 7.5, 30°C
0.59
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant wild-type enzyme, pH 7.5, 30°C
0.11
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin-like serine protease, at 20°C, in 50 mM Tris-HCl buffer, pH 7.5
0.41
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin-like serine protease, at 80°C, in 50 mM Tris-HCl buffer, pH 7.5
2.4
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin, at 20°C, in 50 mM Tris-HCl buffer, pH 8.0
7.9
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin, at 80°C, in 50 mM Tris-HCl buffer, pH 8.0
130
sec-phenethyl alcohol
-
after incubation in 1,4-dioxane, co-lyophilization with methyl-beta-cyclodextrin
319
sec-phenethyl alcohol
-
after a 4-day incubation period in 1,4-dioxane, co-lyophilization with methyl-beta-cyclodextrin
0.28
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 5°C
0.32
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C
0.58
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C, DTT
0.63
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 40°C
2.25
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 75°C
4
Suc-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C
6.67
Suc-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C, DTT
17
Suc-Ala-Ala-p-nitroanilide
-
pH 7.5, 40°C
0.33
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
-
pH 7.5, 5°C or 40°C
0.92
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
-
pH 7.5, 75°C
0.68
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
-
pH 7.5, 5°C
1.01
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
-
pH 7.5, 40°C
3.37
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
-
pH 7.5, 75°C
0.0027
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
1 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
0.0036
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
0.0037
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
0.0037
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
0.43
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
recombinant enzyme, pH 8.5, 22°C
0.8
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-c
1.74
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-g
1.9
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-e and S166S-f
2
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C wild-type
1.9
succinyl-AAPE-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-a
4.4
succinyl-AAPE-p-nitroanilide
-
pH 8.6, 25°C wild-type
0.39
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-a
0.51
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C
0.54
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-d
0.55
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C, wild-type and mutant M222C-S-e
0.6
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-c and S166C-S-f
0.61
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-f
0.63
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-b
0.65
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C
0.67
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-d
0.68
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-b and M222-S-a
0.68
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C, mutant S166C-a
0.69
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-c
0.7
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-e
0.7
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C, mutant S166C-f
0.72
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-c
0.73
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C wild-type
0.74
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-d
0.74
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C, mutant S166C-g
0.77
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C
0.78
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-e
0.79
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-a
0.86
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-f
0.92
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-b
1.09
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-e
1.17
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-c
1.34
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-b
1.5 - 2
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-h
1.6
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-d
2.26
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-i
2.46
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-j
3.4
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-b
5.2
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-j
5.3
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-i
5.5
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-d
7.2
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C wild-type
8.2
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-h
1.2
Succinyl-Ala-Ala-Ala 4-nitroanilide
-
subtilisin Carlsberg
1.5
Succinyl-Ala-Ala-Ala 4-nitroanilide
-
subtilisin DY
0.13
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
-
0.13
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl 7-amide, subtilisin Sendai
0.13
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
mutant subtilisin BPN' Asp to Ser99
0.07
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 5°C
0.15
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 20°C
0.3
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 40°C
0.44
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 20°C, DTT
0.71
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
subtilisin DY
0.79
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
subtilisin Carlsberg
1
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 75°C
30
toluenesulfonyl-Arg methyl ester
-
subtilisin Novo, N-acetyl-Phe methyl ester, subtilisin Carlsberg
40
toluenesulfonyl-Arg methyl ester
-
subtilisin Carlsberg
0.14
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMSO
0.19
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMF
0.25
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMSO
4
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMF
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Km of subtilisin Novo chemically attached to soluble DEAE-dextran and insoluble DEAE-Sephadex
-
additional information
additional information
-
the temperature dependence of the kinetics and thermodynamic parameters suggest that the enzyme exists in two, i.e. cold and hot forms, at 22°C the cold form turns into the hot one possibly owing to a conformational change
-
additional information
additional information
kinetic analysis of the perhydrolytic wild-type and mutant enzyme activities, overview
-
additional information
additional information
-
Michaelis-Menten kinetics using Lineweaver-Burk and Hanes plots at pH 8.5 and 22°C, purified recombinant enzyme
-
additional information
additional information
Michaelis-Menten kinetics and thermodynamics
-
additional information
additional information
-
Michaelis-Menten kinetics using Lineweaver-Burk and Hanes plots
-
additional information
additional information
Michaelis-Menten kinetics, Km is 0.175% w/v
-