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3.4.21.61: Kexin

This is an abbreviated version!
For detailed information about Kexin, go to the full flat file.

Word Map on EC 3.4.21.61

Reaction

Cleavage of -Lys-Arg-/- and -Arg-Arg-/- bonds to process yeast alpha-factor pheromone and killer toxin precursors =

Synonyms

adrenorphin-Gly-generating enzyme, ASP, BLI-4, corin activating enzyme, EC 3.4.22.23, endoproteinase Kex2p, Gene KEX2 dibasic proteinase, HuPCSK6, Kex 2p proteinase, Kex2, Kex2 endopeptidase, Kex2 endoprotease, Kex2 endoproteinase, Kex2 protease, Kex2 proteinase, Kex2-660, Kex2-like endoproteinase, Kex2-like precursor protein processing endoprotease, Kex2p, kexin, More, NARC-1, Paired-basic endopeptidase, PC 1/3, PC1, PC1/3, PC2, PCSK type 9, PCSK6, PCSK9, plasma proprotein convertase subtilisin-kexin type 9, pro-protein convertase subtilisin/kexin type 9, prohormone convertase 1, prohormone convertase 1/3, prohormone convertase 2, prohormone processing protease, Prohormone-processing endoprotease, Prohormone-processing KEX2 proteinase, Prohormone-processing proteinase, Proprotein convertase, proprotein convertase 1/3, proprotein convertase Kex2, proprotein convertase subtilisin kexin type 9, proprotein convertase subtilisin kexin-9, proprotein convertase subtilisin-like/kexin type 9, proprotein convertase subtilisin/kexin 6, proprotein convertase subtilisin/kexin type 6, proprotein convertase subtilisin/kexin type 9, proprotein convertase subtilisin/kexin type 9 (PCSK9)Proprotein convertase subtilisin/kexin type 9, proprotein processing protease, Protease KEX2, Proteinase Kex2p, Proteinase yscF, Proteinase, prohormone-processing, Yeast KEX2 protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.61 Kexin

Cloned

Cloned on EC 3.4.21.61 - Kexin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
adenovirus construct expressing PC1/3
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cDNAs encoding full-length PCSK9 splicing variant or mutants inserted into a modified pJB02 vector with a C-terminal Flag-HIS6 or HIS6 tag, respectively. The resulting constructs used for transient transfection of HEK-293 cells
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cloned and expressed in Pichia pastoris
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cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells
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expressed in HEK-293 cells, COS-1 cells or Y1 cells
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expression in HEK293 cell
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expression in HEK293 cells
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expression in HEK293T and HepG2 cells
full-length and mutant PCSK9-V5-His proteins expressed in HEK-293 cells. Truncated PCSK9DELTAC (Gln-31-Ala-451) proteins with a C-terminal His tag expressed in Escherichia coli BL21 cells
GH4 cells co-transfected with preproGIP and PC1/3
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into vector pET-21a(+)
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isolation of the structural gene
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ligated into vector pCR2.1-Topo and subcloned into vector pET-21a(+)
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overexpressed in HEK-293 cells
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recombinant expression of enzyme mutants in Hep-G2 cells
recombinant His-tagged enzyme catalytic domain expression in HEK-293T cells and secretion to the medium
recombinant overexpression of the human enzyme in mouse LDL-receptor null mutant cells, expression and protein profiles, overview. Overexpression of PCSK9 increases plasma ApoB levels in an LDLR-independent fashion
recombinant PC1/3 produced using the baculovirus expression system in Sf-9 insect cells or through intracoelemic injection in insect larvae
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transformation of the single-gene deletion strain Deltakex2 with pYEX12 plasmid
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wild type and mutant D374Y-FLAG expressed in Hep-G2 cells
wild-type PCSK9 with a V5 epitope flag inserted into pcDNA3.1. PCSK9 mutants expressed in primary hepatocytes derived from PCSK9-/- mice