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3.4.21.59: Tryptase

This is an abbreviated version!
For detailed information about Tryptase, go to the full flat file.

Word Map on EC 3.4.21.59

Reaction

Preferential cleavage: Arg-/-, Lys-/-, but with additional information restricted specificity than trypsin =

Synonyms

alpha 1-tryptase, alpha tryptase, alpha-tryptase, basal serum tryptase, beta tryptase I, beta tryptase II, beta-tryptase, betaI-tryptase, betaII-tryptase, BLCT, bovine liver capsule tryptase, bovine mast cell tryptase, BTM, delta-tryptase, gamma-tryptase, group 2 tryptase, HAST, HMC-1 tryptase, HTbeta, human beta-tryptase, human betaII-tryptase, human lung tryptase, human mast cell tryptase, human mast cell tryptase beta I, human tryptase alpha, human tryptase beta, human tryptase beta/2, human tryptase-beta, Lung tryptase, Mast cell neutral proteinase, mast cell protease 11, mast cell protease 6, mast cell protease 7, Mast cell protease II, mast cell protease-6, Mast cell proteinase II, mast cell specific tryptase, Mast cell tryptase, mast cell tryptase beta, mast cell-derived tryptase, mast cell-specific protease, mast cell-specific tryptase, MC protease 6, MC protease 7, MC protease-6, MC tryptase, MCP-11, MCP-6, MCP-7, Mcpt6, MCT, mMCP-6, mouse mast cell protease 6, mouse mast cell protease-6, Pituitary tryptase, Proteinase, mast cell neutral, Proteinase, mast cell serine, II, Proteinase, mast cell serine, tryptase, Prss31, Rat mast cell protease II, rHT, S-tryptase, sBT, serine protease tryptase, serum baseline tryptase, serum mast cell tryptase, serum tryptase, Skin tryptase, TMT, TPSAB1, TPSB2, transmembrane tryptase, tryptase, tryptase alpha, tryptase alpha-1, tryptase beta, tryptase beta-1, tryptase beta-2, Tryptase M, Tryptase, skin, tryptase-2, tryptase-beta, tryptase-beta1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.59 Tryptase

General Stability

General Stability on EC 3.4.21.59 - Tryptase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of protein to the tryptase-heparin mixture is needed to keep the enzyme stable
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calcium destabilizes
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chondroitin monosulfate stabilizes, prolongs t1/2 at 37°C 20fold
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chondroitin sulfate E stabilizes, prolongs t1/2 at 37°C 69fold
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heparan sufate stabilizes, prolongs t1/2 at 37°C 20fold
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heparin glycosaminoglycan fragments of MW greater than 5700 stabilizes
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heparin glycosaminoglycan partially stabilizes the tryptase
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heparin glycosaminoglycan stabilizes, maximal stabilization at a weight ratio to tryptase equal to or greater than unity
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heparin stabilizes
heparin stabilizes human and dog enzyme
heparin stabilizes the active tetramer
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heparin stabilizes the beta-tryptase structure mainly through salt bridge interaction
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heparin stabilizes the enzyme, retains about 50% activity in a low-salt solution after 2 h incubation at 25°C
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heparin-proteoglycan stabilizes the active enzyme monomers
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In low-salt buffer the enzyme is labile, at least 1.4 M KCl is needed to keep the enzyme stable if incubated at 37°C for 30 min
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increasing the NaCl concentration from 0.01 M to 1.0 M increases stability of free tryptase, in the presence of stabilizing polysaccharides it decreases the stability of tryptase until dissociation of tryptase from each polysaccharide occurs, thereafter tryptase stability increases as does that of free tryptase
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kinetic characterization of spontaneous inactivation
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mature alpha1-tryptase does not require heparin-binding for stability
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poly-D-glutamic acid stabilizes, prolongs t1/2 at 37°C 55fold: Dextran sulfate and heparin provide complete stabilization of tryptase activity for 120 min at 37°C
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stabilized by heparin proteoglycans
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stable to repeated freeze-thaw cycles
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