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3.4.21.50: lysyl endopeptidase

This is an abbreviated version!
For detailed information about lysyl endopeptidase, go to the full flat file.

Word Map on EC 3.4.21.50

Reaction

Preferential cleavage: Lys-/-, including -Lys-/-Pro- =

Synonyms

Achrombacter protease I, Achromobacter lysyl endopeptidase, Achromobacter protease I, Achromobacter proteinase I, achromopeptidase, API, caseinase, endo-Lys-C protease, endopeptidase Lys-C, endoproteinase, endoproteinase Lys-C, endoproteinase lysine-C, KGP, LEP, Lys-C, Lys-C endoproteinase, Lys-specific serine endopeptidase, lysine endoproteinase, lysine specific gingipain, lysine specific protease, lysine specific proteinase, lysine-specific protease, Lysobacter lysyl endoproteinase, lysyl bond specific proteinase, Lysyl endopeptidase, protease I, protease IV, proteinase, Achromobacter lyticus alkaline I, proteinase, lysine specific, proteinase, Pseudomonas lyticus alkaline , I, Ps-1, Pseudomonas aeruginosa lysine -specific protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.50 lysyl endopeptidase

Cloned

Cloned on EC 3.4.21.50 - lysyl endopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene lys-C, recombinant expression gene-optimized enzyme with a short artificial N-terminal pro-peptide MGSK in Escherichia coli strains JM109(DE3) and DSM 14459 from rhamnose-inducible expression vector pJOE to 560.3 U/l activity, the pre-N-pro gene is also cloned into pJOE, resulting in expression vector pNP-2, method optimization to over 58fold increased activity, overview
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gene PGN_1416, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3)
overproduction and secretion into the periplasm of Escherichia coli
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recombinant expression of Gly681-Ala710 deletion mutant in Porphyromonas gingivalis strain ECR368, release of enzyme Kgp catalytic domain in soluble form into the culture supernatant
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the cloned gene of prepro-API is expressed in Escherichia coli, pro-API is secreted into the periplasm and activated autocatalytically
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