3.4.21.48: cerevisin

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For detailed information about cerevisin, go to the full flat file.

Reaction

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt Ac-Tyr-OEt. Does not hydrolyse peptide amides =

Synonyms

baker's yeast proteinase, baker's yeast proteinase B, brewer's yeast proteinase, EC 3.4.22.9, PRB1, Proteinase B, Proteinase YSCB, proteinase, yeast B, yeast proteinase B, yeast proteinase yscB

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.48 cerevisin

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Results	in table
1	Activating Compound
21	AA Sequence
2	Application
1	CAS Registry Number
2	Cloned(Commentary)
1	General Information
6	General Stability
33	Inhibitors
3	KM Value [mM]
10	Localization
12	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
25	Organism
5	pH Optimum
2	pH Range
3	Posttranslational Modification
1	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
24	Reference
6	Specific Activity [micromol/min/mg]
2	Storage Stability
31	Substrates and Products (Substrate)
7	Subunits
13	Synonyms
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Application

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Application on EC 3.4.21.48 - cerevisin

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

synthesis

2 entries

synthesis

Saccharomyces cerevisiae

-

high-level production of human mechano-growth factor in Saccharomyces cerevisiae is interfered by proteinase B. Expression of mechano-growth factor in a proteinase B deletion mutant results in a fivefold increase of yield

718011

synthesis

Saccharomyces cerevisiae

-

much stricter experimental protocols than those routinely used are necessary to prevent the artefactual interpretation of protein levels in strains or conditions that increase proteinase B activity. Stability of some proteins, such as Slt2p or Chs4p, but not others, is severely compromised in the rim101 mutant due to the upregulation of the PRB1 gene. Proteolytic degradation during protein processing can be almost completely prevented by an overdose of subtilisin-like protease inhibitors, such as PMSF, or by avoiding cell freezing

718456