3.4.21.4: trypsin

This is an abbreviated version!
For detailed information about trypsin, go to the full flat file.

Reaction

preferential cleavage: Arg-/-, Lys-/- =

Synonyms

acrosin, alpha-trypsin, anionic salmon trypsin, anionic trypsin, Anionic trypsinogen, AST, aT-I, aT-II, Atlantic cod trypsin I, beta-trypsin, BPT, Brain trypsinogen, BT, cationic trypsin, Cationic trypsinogen, cocoonase, CST, EC 3.4.4.4, EFE, EG-T, fibrinolytic enzyme, gamma-trypsin, GM-T, group III trypsin, mesotrypsin, Mesotrypsinogen, midgut-specific serine protease 1, midgut-specific serine protease 2, More, p23, parenzyme, parenzymol, PPT, pro23, pseudotrypsin, SET, SGT, sperm receptor hydrolase, ST-1, ST-2, ST-3, TI, TIIA, TIII, TLE, TLE I, TLE II, TR-P, TR-S, tripcellim, TRP, Try, TRY-3, TryI, TryII, TryIII, TRYP, trypsin 1, trypsin 3A1, trypsin 4, trypsin A, trypsin B, trypsin I, trypsin IV, trypsin type III, trypsin Y, trypsin-1, trypsin-2, trypsin-3, trypsin-4, trypsin-like enzyme, trypsin-like serine proteinase, tryptar, tryptase, trypure, TrypZean, type I trypsin, type IX pancreatic trypsin

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.4 trypsin

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Results	in table
18	Activating Compound
676	AA Sequence
20	Application
1	CAS Registry Number
24	Cloned(Commentary)
38	Crystallization (Commentary)
12526	Disease/ Diagnostics
1	Expression
20	General Information
28	General Stability
9	IC50 Value
419	Inhibitors
64	kcat/KM [mM/s]
70	Ki Value [mM]
400	KM Value [mM]
3	Localization
34	Metals/Ions
92	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
8	Organic Solvent Stability
308	Organism
1	Oxidation Stability
1	Pathway
853	PDB ID
90	pH Optimum
27	pH Range
41	pH Stability
17	pI Value
18	Posttranslational Modification
44	Protein Variants
80	Purification (Commentary)
3	Reaction
2	Reaction Type
306	Reference
3	Renatured (Commentary)
230	Source Tissue
58	Specific Activity [micromol/min/mg]
6	Storage Stability
618	Substrates and Products (Substrate)
51	Subunits
102	Synonyms
69	Temperature Optimum [°C]
19	Temperature Range [°C]
58	Temperature Stability [°C]
375	Turnover Number [1/s]

Renatured

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

denatured trypsinogen is rapidly diluted with re-folding buffer (0.7 M guanidine-HCl, 0.1 M Tris-HCl, pH 8.0, containing 10 mM benzamidine, 2 mM L-cysteine, and 1 mM L-cystine)

Homo sapiens

-

700478

reversible denaturation at pH 12.0 and at pH 2.0

Streptomyces griseus

-

95441

the denatured trypsin is diluted with 1 M diethanolamine or other solutions to a final concentration of 100 nM at pH 8.2, in the case of fully reduced trypsin, the refolding solution also contains 3 mM glutathione and 1 mM oxidized glutathione, the addition of 1.5 M urea resulted in even less recovered activity despite counteracting the formation of precipitates, refolding from fully reduced trypsin is achieved with a substantial approximate 86% recovery of protein yield

Bos taurus

-

699046