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3.4.21.4: trypsin

This is an abbreviated version!
For detailed information about trypsin, go to the full flat file.

Word Map on EC 3.4.21.4

Reaction

preferential cleavage: Arg-/-, Lys-/- =

Synonyms

acrosin, alpha-trypsin, anionic salmon trypsin, anionic trypsin, Anionic trypsinogen, AST, aT-I, aT-II, Atlantic cod trypsin I, beta-trypsin, BPT, Brain trypsinogen, BT, cationic trypsin, Cationic trypsinogen, cocoonase, CST, EC 3.4.4.4, EFE, EG-T, fibrinolytic enzyme, gamma-trypsin, GM-T, group III trypsin, mesotrypsin, Mesotrypsinogen, midgut-specific serine protease 1, midgut-specific serine protease 2, More, p23, parenzyme, parenzymol, PPT, pro23, pseudotrypsin, SET, SGT, sperm receptor hydrolase, ST-1, ST-2, ST-3, TI, TIIA, TIII, TLE, TLE I, TLE II, TR-P, TR-S, tripcellim, TRP, Try, TRY-3, TryI, TryII, TryIII, TRYP, trypsin 1, trypsin 3A1, trypsin 4, trypsin A, trypsin B, trypsin I, trypsin IV, trypsin type III, trypsin Y, trypsin-1, trypsin-2, trypsin-3, trypsin-4, trypsin-like enzyme, trypsin-like serine proteinase, tryptar, tryptase, trypure, TrypZean, type I trypsin, type IX pancreatic trypsin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.4 trypsin

Inhibitors

Inhibitors on EC 3.4.21.4 - trypsin

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R,4R)-4-phenyl-1-[Nalpha-(7-methoxy-2-naphthalenesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid
competitive, small molecular weight inhibitor without tryptase inhibitor activity
(4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl)-(3,4,5-6-tetrahydro[2H-1,4']bipyridinyl-4yl)-methanone
-
-
1,10-phenanthroline
-
-
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
1-[N2-[(7-methoxynaphthalen-2-yl)sulfonyl]-L-arginyl]-4-phenylpiperidine-2-carboxylic acid
IC50: 0.0003 mM
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
unspecific strong inhibition
2-mercaptoethanol
-
0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity
3,4-dichloroisocoumarin
-
-
4-(2-aminoethyl)-benzensulfonylfluoride
-
isozyme TryIII activity is completely (more than 95%) inhibited by 4-(2-aminoethyl)-benzensulfonylfluoride
4-(amidinophenyl)methanesulfonyl fluoride
-
-
6-amidino-2-naphthol
-
mixed-type inhibition
acetonitrile
-
partial inhibition at 20% acetonitrile, a 5% aqueous acetonitrile solution less likely reduces the activity of the porous polymer monolith-immobilized trypsin
acyclic SFTI
-
i.e. oSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
acyclic sun flower trypsin inhibitor-1
-
-
-
Adenanthera pavonina trypsin inhibitor
-
Adunil CEL
-
0.1% v/v, 47.3% residual activity
-
alpha-1-antitrypsin
-
in gestational diabetes mellitus reduction of serum trypsin inhibitory capacity may be due to non-enzymatic glycosylation of alpha-1-antitrypsin or oxidation of methionine in the active site of alpha-1-antitrypsin
-
alpha1-antitrypsin
-
-
-
alpha1-antitrypsin Pittsburgh
-
effective inhibitor, variant of alpha1-antitrypsin containing an Arg residue in place of the P1 Met358 in the reactive-site peptide bond
-
antipain
Apios Americana trypsin inhibitor
-
AATI, strong inhibitor, complete inhibition with Apios Americana trypsin inhibitor bound to trypsin in a 1:2 molar ratio
-
Aprotinin
atropine
-
strongly diminishes trypsin activity in stressed animals
AVNIPFKVHFRCKAAFC
-
a small trypsin inhibitor from the skin secretion of the frog Odorrana grahami, only inhibits the hydrolysis activity of trypsin on synthetic chromogenic substrate
Bauhinia variegata var. variegata trypsin inhibitor
-
i.e. BvvTI, a Kunitz-type inhibitor from seeds of the Camel’s foot tree, Bauhinia variegata var. variegata. The inhibitor is also capable of significant inhibition of the proliferation of nasopharyngeal cancer CNE-1 cells in a selective way and of inhibiting anti-HIV-1 reverse transcriptase activity, overview
-
benzamidine
benzylamine
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-ethylphenyl)phosphonate
-
50% inhibition at 0.000017 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-isopropylphenyl)phosphonate
-
50% inhibition at 0.000061 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-methylthiophenyl)phosphonate
-
50% inhibition at 0.0000087 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-t-butylphenyl)phosphonate
-
50% inhibition at 0.000088 mM, comparison with inhibitory effect on urokinase
bis(m-amidinosalicylidene-L-alaninato)iron(III)
-
-
bis(p-amidinosalicylidene-L-alaninato)iron(III)
-
-
-
bisphenol A
-
the secondary and tertiary structures of trypsin are altered by bisphenol S binding, which results in the loosening of the skeleton of the enzyme. Bisphenol S induces microenvironmental changes around tyrosine and tryptophan residues of trypsin. The activity of trypsin does not change remarkably with the increasing concentration of bisphenol S. The binding of bisphenol S to trypsin is a spontaneous process and hydrogen bonding and hydrophobic interactions play a vital role in stabilizing the bisphenol S-trypsin complex. The binding constants of bisphenol S with trypsin are 74200 (25°C) and 59100 L/mol (37°C)
black gram trypsin inhibitor 1
-
highly effective trypsin inhibitor, 87.5% of trypsin inhibitory activity of black gram trypsin inhibitor 1 is retained after treatment with 10 mM dithiothreitol for 2 hours, and the activity dwindles to 12.5% after treatment with 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 2
-
highly effective trypsin inhibitor, the trypsin inhibitory activity of black gram trypsin inhibitor 2 is unaffected after exposure to 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 3
-
highly effective trypsin inhibitor, in the presence of 10 mM dithiothreitol trypsin inhibitory of black gram trypsin inhibitor 3 drops to 57.1% after 1 h and becomes undetectable after 2 hours, while treatment of 100 mM dithiothreitol for 1 h has the same effect
-
Bovine pancreatic trypsin inhibitor
-
bovine secretory inhibitors
-
A and B
-
Bowman-Birk inhibitor
BPTI-20st
-
simplified variant of bovine pancreatic trypsin inhibitor containing 20 alanines, retains a wild type level of trypsin inhibitory activity
-
BPTI-[5,55]st
-
single-disulfide-bonded variant of bovine pancreatic trypsin inhibitor, retains a wild type level of trypsin inhibitory activity
-
captopril
-
feeding angiotensin converting enzyme inhibitor captopril down-regulates trypsin activity in the larval gut, injecting third instar larvae with a combination of Aedes aegypti trypsin-modulating oostatic factor and captopril downregulates trypsin biosynthesis in the larval gut
Cd2+
-
10 mM, 48% inhibition
chymostatin
clispin
-
a Clitocybe nebularis trypsin inhibitor variant, recombinantly expressed in Escherichia coli, highly specific towards trypsin, fast-acting, and tight-binding. Molecular cloning of the gene and cDNA encoding cnispin, overview
Clitocybe nebularis trypsin inhibitor
-
CnSPI, several inhibitor variants, separation by trypsin affinity chromatography. Possible regulatory role for CnSPIs in the endogenous proteolytic system of Clitocybe nebularis. Isolation from frozen fruiting bodies or basidiocarps
-
cMCoTI-II
-
-
-
cyclic SFTI
-
i.e. cSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
dithiothreitol
-
1 mM, no residual activity
DMTI-II
-
trypsin inhibitor from seeds of Dimorphandra mollis. The inhibitory activity is stable over a wide range and in the presence of DTT
-
domesticated soybean inhibitor
-
SBTI
-
DOTA-SFTI
-
modified sunflower trypsin inhibitor 1, SFTI-1, labeled, generated by Fmoc-based automated synthesis
E64
-
weak inhibition
Ecballium elaterium trypsin inhibitor II
-
EETI, a a model member of the knottin family, used for construction of libraries of EETI loop-substituted variants with diversity in both amino acid sequence and loop length, wild-type EETI or loop-substituted clones expressed from yeast, structure-function analysis, modelling, overview
-
enalapril
-
feeding angiotensin converting enzyme inhibitor enalapril down-regulates trypsin activity in the larval gut
ethylenediaminetetraacetic acid
-
partial
Gly-L-Arg-(2-amino butanoyl)-L-Thr-Nphe-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp
-
-
Gly-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
sun flower trypsin inhibitor SFTI-1
Glycine max trypsin inhibitor
-
from raw soymilk, inhibition kinetics, overview
-
I-ovalbumin
-
high affinity interaction of enzyme with I-ovalbumin, the product of a heating transition of ovalbumin which acts as a potent reversible serine proteinase inhibitor. Interaction is characterized by high kinetic association constants and low kinetic dissociation konstants
-
iodoacetic acid
16.5% inhibition at 1 mM; 1 mM, 16.5% inhibition
K+
-
10 mM, 16% inhibition
Kunitz domain 1 of tissue factor pathway inhibitor-2
-
-
-
Kunitz type trypsin inhibitor from Glycine max
-
isolated and purified from Korean large black soybeans cultivar, overview. Inhibits bovine trypsin, but also shows Anti-HIV reverse transcriptase activity, cytokine-inducing activity and antiproliferative activity on tumor cell lines
-
L-1-chloro-3-tosylamido-4-phenyl-2-butanone
-
-
L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone
-
-
L-Lys-L-Cys-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
-
leech-derived tryptase inhibitor
-
i.e. LDTI, purely competitive inhibitor
-
LEKTI
-
the LEKI gene encodes a 15-domain serine proteinase inhibitor, disulfide bonds are important for LEKTI function, noncompetitive mechanism, expression and purification of human LEKTI gene in Sf9
leupeptin
Li+
-
10 mM, 19% inhibition
lily bulb trypsin inhibitor
-
-
-
Lima bean trypsin inhibitor
-
LKGCWTKSIPPKPCFGK
-
-
Locusta migratoria protease inhibitor 1
-
strong inhibition
-
Locusta migratoria protease inhibitor 3
-
strong inhibition, the inhibitor interacts with trypsin through the reactive site P3-P4' and the P10-P6 residues
-
low-molecular-mass trypsin inhibitor inhibitor type-2
-
from Sinapis alba, inhibitor consists of a peptide mixture, displaying Ile or Arg at position 43, Trp or kynurenine at position 44, and C-terminal ragged ends
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride
-
-
-
m-guanidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
mung bean trypsin inhibitor
-
mustard trypsin inhibitor 2
-
mustard trypsin inhibitor II
inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
N-alpha-p-tosyl-L-lysine chloromethyl ketone
N-alpha-tosyl-L-Lys-chloromethyl ketone
-
complete inhibition at 1 mM
N-alpha-tosyl-L-lysine chlormethylketone
-
less than 50% inhibition of isozyme TryIII by the trypsin-specific inhibitor N-alpha-tosyl-L-lysine chlormethylketone
N-alpha-tosyl-L-lysine chloromethyl ketone
-
-
N-alpha-tosyl-L-lysine-chloromethylketone
-
complete inhibition at 5 mM
N-p-tosyl-L-lysine chloromethyl ketone
N-p-tosyl-lysine chloroketone
N-tosyl-L-lysine chloromethyl ketone
82.9% inhibition at 5 mM
N-tosyl-L-lysine-chloromethyl ketone
N-tosyl-L-phenyl-alanine-chloromethyl ketone
-
11% inhibition at 1mg/ml
N-tosyl-L-phenylalanine-chloromethyl ketone
-
5.34% inhibition at 5 mM
Na+
-
10 mM, 21% inhibition
Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
-
inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
Nafamostat
-
potent inhibitor
nafamostat mesilate
IC50: 0.00003 mM
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-acetyl-piperazide
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-methylpiperidide
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid methyl ester
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid methylester
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-piperazide
-
-
Nalpha-p-tosyl-L-lysine chloromethyl ketone
-
complete inhibition
Nalpha-tosyl-L-Lys-chloromethyl ketone
Nalpha-tosyl-L-Lys-chloromethylketone
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
-
napsagatran
-
-
napsagatran ethylester
-
-
nexin-1
-
nexin-1 inhibits isozymes trypsin-1 ad trypsin-2, nexin-1 inhibits trypsin-4 and forms stable complexes only with this trypsin isoenzyme
-
oMCoTI-II
-
-
-
ovomucoid
-
p-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
p-nitrophenyl-p'-guanidino-benzoate
-
irreversible trypsin inhibitor
p-tosyl-L-Lys-chloromethane
pancreatic secretory trypsin inhibitor
-
Pancreatic trypsin inhibitor
-
PdKI-3.1
-
PdKI-3.2
-
pefabloc
-
-
Pefabloc SC
pepstatin
-
-
pepstatin A
26% inhibition at 0.01 mM
Phaseolus vulgaris trypsin inhibitor
-
two different variants of 132 kDa and 118 kDA from navy beans and red kidney beans, respectively, isolated by heat treatment and ammonium sulfate fractionation
-
phenyl methyl sulphonyl fluoride
-
100% inhibition
phenylbutylamine
Phenylethylamine
phenylmethyl sulfonyl fluoride
phenylmethylsulfonyl fluoride
Phenylmethylsulfonylfluoride
Phenylpropylamine
Plathymenia foliolosa trypsin inhibitor
-
PFTI
-
polylysine
-
immobilized enzyme
porcine secretory inhibitors
-
-
-
porcine trypsin inhibitor
-
0.01 mM, more than 95% inhibition
-
potato inhibitor II
strong inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
Protease Inhibitor Cocktail
-
-
-
Putranjiva roxburghii trypsin inhibitor
-
highly potent inhibitor of bovine trypsin, trypsin inhibitory activity of Putranjiva roxburghii trypsin inhibitor is completely retained up to 70°C, above 70°C, there is a slight decrease in the inhibitory activity retaining almost 85% inhibitory activity up to 80°C, the inhibitory activity of Putranjiva roxburghii trypsin inhibitor falls sharply above 80°C with a loss of almost 80% inhibitory activity at 90°C, only a slight decrease of 5% in inhibitory activity is observed when Putranjiva roxburghii trypsin inhibitor is incubated for 2 h at 100 mM dithiothreitol
-
Sagittaria sagittifolia arrowhead protease inhibitor A
-
API-A
-
small glossy black soybean trypsin inhibitor
-
inhibitory activity is stable in the pH range 3-13 and in the temperature range 0-60°C, is inhibited by dithiothreitol (5-25 mM) in a dose-dependent manner
-
sodium dodecylsulfate
-
0.5% w/v, no residual activity
soybean Bowman-Birk inhibitor
inhibition of HzTrypsin-C,no inhibition of HzTrypsin-S
-
soybean Kunitz trypsin inhibitor
-
Soybean trypsin inhibitor
-
Spinacia oleracea trypsin inhibitor
-
high affinity between trypsin and Spinacia oleracea trypsin inhibitor
-
sun flower trypsin inhibitor-1
-
-
sunflower trypsin inhibitor 1
-
SFTI-1, binding and effects on human prostate cancer cells, overview
sunflower trypsin inhibitor-1
SVIGCWTKSIPPRPCFVK-amide
-
HV-BBI is a Bowman-Birk type protease inhibitor from the skin secretion of the Chinese Bamboo odorous frog Huia versabilis, SVIGCWTKSIPPRPCFVK-amide is a synthetic replicate of HV-BBI with the wild-type K (Lys8) residue in the presumed P1 position and is a potent, competitive, and reversible inhibitor of trypsin
SVIGCWTRSIPPRPCFVK-amide
-
HV-BBI is a Bowman-Birk type protease inhibitor from the skin secretion of the Chinese Bamboo odorous frog Huia versabilis, SVIGCWTRSIPPRPCFVK-amide is a synthetic replicate of HV-BBI with the mutant R (Arg8) residue in the presumed P1 position and is a competitive, and reversible inhibitor of trypsin with reduced potency compared to the wild type peptide SVIGCWTKSIPPRPCFVK-amide
T5E/N18R/T20G/P21S/T22D/K31M mutant Schistocerca gregaria protease (trypsin) inhibitor 1
-
the mutant inhibitor is improved compared to the wild-type inhibitor protein
-
Tamarindus indica trypsin inhibitor
-
a Kunitz-type inhibitor
-
thionine
-
from cowpea, inhibits trypsin, no inhibition of chymotrypsin
tissue inhibitor of matrix metalloproteinases-2
-
TIMP-2, prevents the C-terminal truncation of activated metalloproteinase-2, without affecting the generation of the initial 62 kDa activated species
-
tosyl-L-lysine-chloromethyl ketone
-
-
tosyl-L-lysyl chloromethyl ketone
tosyl-L-Phe chloromethyl ketone
-
-
TPI-1
-
-
-
TPI-2
-
-
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane
-
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
-
trypsin inhibitor I-S Type from soybean
-
reversible trypsin inhibitor
-
trypsin modulating oostatic factor
-
the hexapeptide NPTNLH is secreted at the last stage of vitellogenesis and inhibits the biosynthesis of trypsin in insect midgut cells
-
tumor-associated trypsin inhibitor
-
ulinastatin
-
-
urinary trypsin inhibitor
-
Vigna angularis trypsin inhibitor
-
from adzuki beans, 13 kDa, isolated by heat treatment and ammonium sulfate fractionation
-
Vigna mungo trypsin inhibitor
-
purification of the Bowman-Birk proteinase inhibitor from the seeds of black gram, Vigna mungo cv. TAU-1. 8041.5 Da by mass spectrometry, pI 4.3-6.0, stable up to 80°C and at pH 2.0-12.0, analysis of the secondary structural conformation, overview, exhibts non-competitive-type inhibitory activity against both bovine pancreatic trypsin
-
wild-type soybean inhibitor
-
WBTI
-
zeamatin
-
-
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
-
[Abu(3, 11)]-sun flower trypsin inhibitor-1
-
-
-
[Arg5]-SFTI-1
-
a wild-type SFTI-1 analogue
[Lys5]-SFTI-1
-
a wild-type SFTI-1 analogue
[N,N'-bis(m-amidinosalicylidene)ethylenediaminato]copper(II)
-
-
-
[N,N'-bis(m-guanidinosalicylidene)ethylenediaminato]copper(II)
-
-
-
[Phe5]-SFTI-1
-
a wild-type SFTI-1 analogue
additional information
-