3.4.21.4: trypsin

This is an abbreviated version!
For detailed information about trypsin, go to the full flat file.

Reaction

preferential cleavage: Arg-/-, Lys-/- =

Synonyms

acrosin, alpha-trypsin, anionic salmon trypsin, anionic trypsin, Anionic trypsinogen, AST, aT-I, aT-II, Atlantic cod trypsin I, beta-trypsin, BPT, Brain trypsinogen, BT, cationic trypsin, Cationic trypsinogen, cocoonase, CST, EC 3.4.4.4, EFE, EG-T, fibrinolytic enzyme, gamma-trypsin, GM-T, group III trypsin, mesotrypsin, Mesotrypsinogen, midgut-specific serine protease 1, midgut-specific serine protease 2, More, p23, parenzyme, parenzymol, PPT, pro23, pseudotrypsin, SET, SGT, sperm receptor hydrolase, ST-1, ST-2, ST-3, TI, TIIA, TIII, TLE, TLE I, TLE II, TR-P, TR-S, tripcellim, TRP, Try, TRY-3, TryI, TryII, TryIII, TRYP, trypsin 1, trypsin 3A1, trypsin 4, trypsin A, trypsin B, trypsin I, trypsin IV, trypsin type III, trypsin Y, trypsin-1, trypsin-2, trypsin-3, trypsin-4, trypsin-like enzyme, trypsin-like serine proteinase, tryptar, tryptase, trypure, TrypZean, type I trypsin, type IX pancreatic trypsin

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.4 trypsin

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Results	in table
18	Activating Compound
676	AA Sequence
20	Application
1	CAS Registry Number
24	Cloned(Commentary)
38	Crystallization (Commentary)
12526	Disease/ Diagnostics
1	Expression
20	General Information
28	General Stability
9	IC50 Value
419	Inhibitors
64	kcat/KM [mM/s]
70	Ki Value [mM]
400	KM Value [mM]
3	Localization
34	Metals/Ions
92	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
8	Organic Solvent Stability
308	Organism
1	Oxidation Stability
1	Pathway
853	PDB ID
90	pH Optimum
27	pH Range
41	pH Stability
17	pI Value
18	Posttranslational Modification
44	Protein Variants
80	Purification (Commentary)
3	Reaction
2	Reaction Type
306	Reference
3	Renatured (Commentary)
230	Source Tissue
58	Specific Activity [micromol/min/mg]
6	Storage Stability
618	Substrates and Products (Substrate)
51	Subunits
102	Synonyms
69	Temperature Optimum [°C]
19	Temperature Range [°C]
58	Temperature Stability [°C]
375	Turnover Number [1/s]

Activating Compound

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of the Enzyme Summary Page.

Activating Compound on EC 3.4.21.4 - trypsin

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

alkylbenzene sulfonate

Oreochromis niloticus

-

1% v/v, 257% of initial activity

731844

-

caerulein

Homo sapiens

-

trypsinogen activation to trypsin occurs with 10 nM caerulein, trypsinogen is activated to reach a trypsin level of 6 nM following 10 nM caerulein induction for 1 h

696328

dithiothreitol

Drosophila melanogaster

-

addition of 2 mM dithiothreitol greatly enhances the tryptic activity

698277

EDTA

Sus scrofa

-

increase in activation of pro-matrix metalloproteinase-2

669859

enterokinase

2 entries

-

enteropeptidase

Bos taurus

-

trypsinogen is activated through the cleavage of a peptide Val-Asp-Asp-Asp-Asp-Lys-Ile located on the N-terminus of the protein, the heptapetide is cleaved autocatalytically by its physiological activator, enteropeptidase

699194

-

methyl-alpha-D-galactoside

2 entries

methyl-alpha-D-mannoside

2 entries

Na+

Streptomyces griseus

P00775

wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa

667718

additional information

6 entries

-

enterokinase

Bos taurus

-

trypsin is activated with 100 U/l enterokinase

698513

-

enterokinase

Homo sapiens

-

trypsinogens (0.0002 mM) are incubated at room temperature with 6-200 ng enterokinase in 0.1 M Tris (pH 8), containing 5 mM CaCl2 and 0.01% bovine serum albumin for 10-60 min

700478

-

methyl-alpha-D-galactoside

Bos taurus

-

1.2fold enhancement of activity at 0.2 M

680742

methyl-alpha-D-galactoside

Sus scrofa

-

1.2fold enhancement of activity at 0.2 M

680742

methyl-alpha-D-mannoside

Bos taurus

-

1.4fold enhancement of activity at 0.2 M

680742

methyl-alpha-D-mannoside

Sus scrofa

-

1.4fold enhancement of activity at 0.2 M

680742

additional information

Bos taurus

-

increase in catalytic efficiency of the trypsin reaction with decreasing dielectric constant results from the stabilization of electrostatic energy for the formation of an oxyanion hole, and this stabilization is caused by the increase of electrostricted water around the charged tetrahedral transition state. The control of the solvent dielectric constant can stabilize the tetrahedral transition state, and this lowers the activation energy

650362

-

additional information

Bos taurus

-

activity is not enhanced in 0.2 M lactose

680742

-

additional information

Bos taurus

-

pancreatic trypsin activity increases linearly with increasing dietary crude protein concentration

698513

-

additional information

Homo sapiens

-

tryptase expression in human skin is significantly increased at 24 and 48 h postirradiation with ultraviolet, infrared, and heat treatment

697021

-

additional information

Mus musculus

-

colonic trypsin activity increases during acute stress

699942

-

additional information

Sus scrofa

-

activity is not enhanced in 0.2 M lactose

680742

-