Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.37: leukocyte elastase

This is an abbreviated version!
For detailed information about leukocyte elastase, go to the full flat file.

Word Map on EC 3.4.21.37

Reaction

Hydrolysis of proteins, including elastin. Preferential cleavage Val-/- > Ala-/- =

Synonyms

bone marrow serine protease, EC 3.4.21.11, EC 3.4.4.7, ELA2, Elane, elastase, elaszym, granulocyte elastase, H2A specific protease, H2Asp, HLE, HNE, leukocyte elastase, lysosomal elastase, N-elastase, NE, neutrophil elastase, neutrophil-elastase, neutrophil-released elastase, PMN elastase, PMN-elastase, PMNE, polymorphonuclear leukocyte elastase, polymorphonuclear neutrophil elastase, serine elastase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.37 leukocyte elastase

Crystallization

Crystallization on EC 3.4.21.37 - leukocyte elastase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, 2.3 A crystal structure of leukocyte elastase in a complex with methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone
complex of the enzyme with methoxysuccinyl-Ala-Ala-Pro-Ala chloromethyl ketone. The asymmetric distribution of positively charged residues on the surface of the enzyme suggests a mechanism for the specific incorporation of the enzyme into primary granules in the neutrophil
crystallized in complex with Pro44-eglin c, hanging drop vapour diffusion method using polyethylene glycol 4000 as the precipitating agent
-
hanging drop vapour diffusion method
-
hanging drop vapour diffusion method, using 0.1 M HEPES (pH 7.5) and 20% PEG8000 or in 0.1 M HEPES (pH 7.5) and 35% PEG8000 containing 10 mM N-methoxysuccinyl-Ala-Ala-Pro-Val chloromethyl ketone inhibitor
-
in complex with 1a, sitting drop vapor diffusion method, using 20% (w/v) PEG MME 5000, 0.2 M sodium sulfate
in complex with dihydropyrimidone inhibitor, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M ammonium citrate (pH 5.0) or uncomplexed enzyme, sitting drop vapor diffusion method, using 1.9 M ammonium sulfate, 5% (w/v) PEG 400, 0.1 M 4-morpholine ethanesulfonic acid/NaOH (pH 6.5), and 20% (v/v) glycerol
in complex with the recombinant C-terminal domain of secretory leukocyte protease inhibitor (Arg58Ala107), sitting drop vapour diffusion method, using 2.0 M Na-formate at pH 4.5
-
X-ray crystal structure of the complex of human leukocyte elastase and the third domain of the turkey ovomucoid inhibitor