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0.19
benzyloxycarbonyl-Arg-p-nitrobenzyl ester
-
-
1.2 - 14
D-Ile-Pro-Arg-4-nitroanilide
0.85 - 10.4
D-Ile-Pro-Arg-p-nitroanilide
1.5 - 9.8
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
0.67
methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide
-
wild-type enzyme
0.8 - 50
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
11.8
S-2288
-
in 50 mM HEPES, pH 7.4, with 0.1 M NaCl, 5 mM CaCl2, 0.1% (w/v) bovine serum albumin, and 0.01% Tween 80, at 37°C
0.67
spectrozyme fVIIa
-
wild-type enzyme
additional information
additional information
-
1.2
D-Ile-Pro-Arg-4-nitroanilide
wild type enzyme, in the presence of soluble tissue factor (1-219), at pH 7.4 and 25°C
1.8
D-Ile-Pro-Arg-4-nitroanilide
wild-type FVIIa + soluble tissue factor for induced allosteric activation of factor VIIa
7.1
D-Ile-Pro-Arg-4-nitroanilide
mutant G372A + soluble tissue factor for induced allosteric activation of factor VIIa, pH 7.4
8.9
D-Ile-Pro-Arg-4-nitroanilide
wild type enzyme, in the absence of soluble tissue factor (1-219), at pH 7.4 and 25°C
10
D-Ile-Pro-Arg-4-nitroanilide
wild-type FVIIa, pH 7.4
14
D-Ile-Pro-Arg-4-nitroanilide
mutant G372A, pH 7.4
0.85
D-Ile-Pro-Arg-p-nitroanilide
-
mutant FVIIaVEAY-sTF
1.25
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, plasma derived wild-type enzyme, activated by tissue factor
1.43
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, activated wild-type enzyme, activated by tissue factor
1.53
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, mutant enzyme DELTA360-329, without tissue factor
1.6
D-Ile-Pro-Arg-p-nitroanilide
-
wild type FVIIa-sTF
1.7
D-Ile-Pro-Arg-p-nitroanilide
-
mutant FVIIaIIa-sTF
1.73
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, activated by tissue factor
1.9
D-Ile-Pro-Arg-p-nitroanilide
-
mutant FVIIaVEAY
2.22
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, activated wild-type enzyme, without tissue factor
2.82
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, plasma derived wild-type enzyme, without tissue factor
2.98
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, mutant enzyme DELTA360-329, activated by tissue factor
3.06
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, without tissue factor
6.7
D-Ile-Pro-Arg-p-nitroanilide
-
mutant FVIIaIIa
10.4
D-Ile-Pro-Arg-p-nitroanilide
-
wild type FVIIa
0.000012
Factor X
mutant G372A + lipidated tissue factor for induced allosteric activation of factor VIIa, pH 7.4
-
0.000015
Factor X
wild-type FVIIa + lipidated tissue factor for induced allosteric activation of factor VIIa, pH 7.4
-
0.0000184
Factor X
-
pH 7.8
-
0.000043
Factor X
-
mutant enzyme L144A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
-
0.000051
Factor X
-
mutant enzyme L192A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
-
0.000059
Factor X
-
mutant enzyme E154A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
-
0.000069
Factor X
-
wild-type enzyme VIIa, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
-
0.00071
Factor X
-
mutant enzyme M306D
-
0.00105
Factor X
-
wild-type enzyme
-
0.0018
Factor X
-
mutant FVIIaIIa
-
0.0019
Factor X
-
pH 7.4, 37°C, mutant enzyme L305V/K337A
-
0.0019
Factor X
wild-type FVIIa + soluble tissue factor for induced allosteric activation of factor VIIa, pH 7.4
-
0.002
Factor X
-
pH 7.4, 37°C, mutant enzyme K337A
-
0.0021
Factor X
-
pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/L305V/L337
-
0.0021
Factor X
-
mutant FVIIaIIa-sTF
-
0.0021
Factor X
-
mutant FVIIaVEAY-sTF
-
0.0023
Factor X
-
pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q
-
0.0024
Factor X
-
pH 7.4, 37°C, mutant enzyme M298Q
-
0.0024
Factor X
-
pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/K337A
-
0.0027
Factor X
-
pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/L305V
-
0.0027
Factor X
-
wild-type enzyme, in absence of tissue factor
-
0.0027
Factor X
-
mutant FVIIaVEAY
-
0.0029
Factor X
-
pH 7.4, 37°C, wild-type enzyme
-
0.0029
Factor X
-
mutant enzyme L305V, in absence of tissue factor
-
0.0029
Factor X
-
pH 7.4, 37°C, mutant enzyme K305V
-
0.003
Factor X
-
mutant enzyme L305V/M306D/D309S, in absence of tissue factor
-
0.003
Factor X
-
wild type FVIIa-sTF
-
0.003
Factor X
wild-type FVIIa, pH 7.4
-
0.0032
Factor X
mutant G372A + soluble tissue factor for induced allosteric activation of factor VIIa, pH 7.4
-
0.0044
Factor X
-
wild type FVIIa
-
0.0054
Factor X
mutant G372A, pH 7.4
-
0.0054
Factor X
-
in 50 mM HEPES, pH 7.4, with 0.1 M NaCl, 5 mM CaCl2, 0.1% (w/v) bovine serum albumin, and 0.01% Tween 80, at 25°C
-
0.0124
Factor X
-
wild-type enzyme VIIa, tissue factor/phosphatidylcholine vesicles
-
0.0153
Factor X
-
mutant enzyme K192A, tissue factor/phosphatidylcholine vesicles
-
0.0207
Factor X
-
mutant enzyme L144A, tissue factor/phosphatidylcholine vesicles
-
0.023
Factor X
-
100 nM FVIIa, incubation for 20 min at room temperature
-
0.13
Factor X
-
37°C, mutant enzyme L144A
-
0.15
Factor X
-
37°C, mutant enzyme N100A
-
0.17
Factor X
-
37°C, mutant enzyme H101A
-
0.18
Factor X
-
37°C, mutant enzyme D186A
-
0.18
Factor X
-
37°C, mutant enzyme R147A
-
0.21
Factor X
-
37°C, wild-type enzyme
-
0.22
Factor X
-
37°C, mutant enzyme F256A
-
0.22
Factor X
-
37°C, mutant enzyme N100A/H101A/Y179A/F256A
-
0.23
Factor X
-
37°C, mutant enzyme Y179A
-
0.25
Factor X
-
37°C, mutant enzyme L144A/R147A/D186A
-
61
Factor X
-
pH 7.4, 37°C, wild-type enzyme
-
68
Factor X
-
pH 7.4, 37°C, mutant enzyme
-
1.5
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
wild type enzyme, in the presence of tissue factor, pH 7.4
2
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
mutant enzyme L280I/V299M-FVIIa, in the presence of tissue factor, pH 7.4
2.2
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
mutant enzyme C164V/V299C FVIIa, in the presence of tissue factor, pH 7.4
2.3
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
mutant enzyme V299M-FVIIa, in the presence of tissue factor, pH 7.4
6.6
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
mutant enzyme L280I/V299M-FVIIa, pH 7.4
8
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
wild type enzyme, pH 7.4
9.2
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
mutant enzyme C164V/V299C, pH 7.4
9.8
H-D-isoleucyl-L-prolyl-arginine-p-nitroanilide
-
mutant enzyme V299M-FVIIa, pH 7.4
0.8
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, wild-type enzyme, with addition of tissue factor
0.9
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, mutant enzyme V21N/E154I/M156Q, with addition of tissue factor
1.1
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 37°C, wild-type enzyme, addition of tissue factor
1.2
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 37°C, mutant enzyme M156Q, addition of tissue factor
2.2
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 6.5, MTE buffer, without tissue factor
2.3
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 6.5, MES buffer, without tissue factor
3.1
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, mutant enzyme V21N/E154I/M156Q, without tissue factor
5.3
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 37°C, mutant enzyme M156Q
5.5
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, wild-type enzyme, without tissue factor
6.3
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 7.5, Tris buffer, without tissue factor
6.4
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 9.5, BTP buffer, without tissue factor
6.8
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 9.5, ethanolamine buffer, without tissue factor
7.6
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 7.4, 37°C
8
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 7.5, HEPES buffer, without tissue factor
10
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 7.5, MTE buffer, without tissue factor
13
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 9.5, MTE buffer, without tissue factor
50
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 9.5, veronal buffer, without tissue factor
additional information
additional information
-
the KM-value for spectrozyme fVIIa for the mutant enzyme A294V is above 2.5 mM
-
additional information
additional information
-
the ratio of turnover number to KM-value for the substrate methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide is 25000 for wild-type enzyme, 500 for mutant enzyme V154G and 2500 for mutant enzyme V154A
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes with different substrates, overview
-