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3.4.21.115: infectious pancreatic necrosis birnavirus Vp4 peptidase

This is an abbreviated version!
For detailed information about infectious pancreatic necrosis birnavirus Vp4 peptidase, go to the full flat file.

Word Map on EC 3.4.21.115

Reaction

cleaves the (Ser/Thr)-Xaa-Ala-/-(Ser/Ala)-Gly motif in the polyprotein NH2-pVP2-VP4-VP3-COOH of infectious pancreatic necrosis virus at the pVP2-VP4 and VP4-VP3 junctions =

Synonyms

avian infectious busrsal disease birnavirus Vp4 endopeptidase, blotched snakehead birnavirus endopeptidase, Drosophila X virus Vp4 protease, equine arterivirus serine peptidase, infectious pancreatic necrosis birnavirus Vp4 protease, NS, NS protease, S50.001, VP4

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.115 infectious pancreatic necrosis birnavirus Vp4 peptidase

Engineering

Engineering on EC 3.4.21.115 - infectious pancreatic necrosis birnavirus Vp4 peptidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P774Z
18 residues removed from the C-terminus
D537Q
mutant with wild-type activity
D585I
mutant with wild-type activity
D595L
mutant with wild-type activity
D601S
mutant with wild-type activity
D644I
mutant with wild-type activity
D660G/D661S
mutant with wild-type activity
D672N
mutant with wild-type activity
D693L
reduced activity for the VP4-VP3 junction
H547S
reduced activity for the VP4-VP3 junction
H697L
reduced activity for the VP4-VP3 junction
H704S
mutant with wild-type activity
K674A
two recombinant constructs VP4_524-716-K674A and VP4_514-716-K674A generated by site-directed mutagenesis
S633C
40% reduction of VP2-VP4 cleavage, 80% reduction of VP4-VP3 cleavage
H681F
-
amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis
I543G
-
amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis
K674D
-
amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis
S633P
-
amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis
V686Q
-
amino acid substitutions generated in VP4 of MABV by site-directed mutagenesis