Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.113: pestivirus NS3 polyprotein peptidase

This is an abbreviated version!
For detailed information about pestivirus NS3 polyprotein peptidase, go to the full flat file.

Word Map on EC 3.4.21.113

Reaction

Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites =

Synonyms

border disease virus NS3 endopeptidase, BVD NS3 endopeptidase, BVDV NS3 endopeptidase, classical swine fever virus NS3 endopeptidase, CSFV NS3, CSFV NS3 endopeptidase, flavivirus NS3 protease, nonstructural protein 3, nonstructural protein NS3, NS2B/NS3 protease, NS3, NS3 protease, NS3 protein, NS3 proteinase, NS3 serine protease, NS3-4A protease, NS3-SP, NS3prohelx, P80pestivirus P80 protein, pestivirus NS3 protease, S31.001, viral diarrhea virus endopeptidase, WNV NS2B/NS3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.113 pestivirus NS3 polyprotein peptidase

Purification

Purification on EC 3.4.21.113 - pestivirus NS3 polyprotein peptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography
-
on nickel column and by gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage through TEV protease
using HiTrap Co2+-chelating chromatography