3.4.21.110: C5a peptidase

This is an abbreviated version!
For detailed information about C5a peptidase, go to the full flat file.

Reaction

the primary cleavage site is at His67-/-Lys68 in human C5a with a minor secondary cleavage site at Ala58-/-Ser59 =

Synonyms

C5a peptidase, GBS, GBS C5a peptidase, S08.020, SCFI, SCPA, ScpB, scpI, SCPZ, SEZ C5a peptidase, streptococcal chemotactic factor inactivator, surface-bound C5a peptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.110 C5a peptidase

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Results	in table
1	Activating Compound
679	AA Sequence
18	Application
1	CAS Registry Number
11	Cloned(Commentary)
2	Crystallization (Commentary)
2	Expression
5	General Information
13	Inhibitors
3	KM Value [mM]
2	Localization
1	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
30	Organism
5	PDB ID
4	pH Optimum
2	pH Range
1	Posttranslational Modification
18	Protein Variants
7	Purification (Commentary)
1	Reaction
12	Reaction Type
30	Reference
1	Specific Activity [micromol/min/mg]
2	Storage Stability
40	Substrates and Products (Substrate)
35	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 3.4.21.110 - C5a peptidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D130A/S512A

Streptococcus agalactiae

-

SCP mutant with 949-residue fragment

676783

D130A

2 entries

D130A/S512A

Streptococcus pyogenes

-

no hydrolysis of substrate

677242

H193A

2 entries

L512A

Streptococcus pyogenes

-

mutant with reduced proteolytic activity

651655

N295A

2 entries

S512A

2 entries

D130A

Streptococcus sp.

-

strain 90 226 SCPA-mutant, impaired capacity to infect nasal mucosa-associated lymphoid tissue

673982

additional information

6 entries

D130A

Streptococcus pyogenes

-

mutant with reduced proteolytic activity

651655

D130A

Streptococcus pyogenes

B6ETQ5

residue is not critical for catalytic activity

755385

H193A

Streptococcus pyogenes

B6ETQ5

complete loss of activity

755385

H193A

Streptococcus pyogenes

-

mutant with reduced proteolytic activity

651655

N295A

Streptococcus pyogenes

-

mutant with reduced proteolytic activity

651655

N295A

Streptococcus pyogenes

B6ETQ5

residue is not critical for catalytic activity

755385

S512A

Streptococcus pyogenes

-

no autocatalytic cleavage

651137

S512A

Streptococcus pyogenes

B6ETQ5

residue is not critical for catalytic activity

755385

additional information

Streptococcus iniae

-

analysis of M-like protein (simA) and C5a peptidase, two gene homologues of Streptococcus iniae, through allelic replacement reveals that M-like protein plays a significant role in Streptococcus iniae virulence and C5a peptidase does not

700885

additional information

Streptococcus pyogenes

-

isogenic scpA-deficient mutant strain TR-9

674559

additional information

Streptococcus pyogenes SSI-9

-

isogenic scpA-deficient mutant strain TR-9

-

additional information

Streptococcus sp.

-

isogenic M1-mutant, expressses SCPA on cell surface

673973

additional information

Streptococcus sp.

-

mutant ScpBdelta, a common natural variant of ScpB, contains a 4-amino-acid deletion that eliminates peptidase activity. High affinity for fibronectin is maintained in ScpBdelta. ScpB and scpBdelta can complement the fibronectin-binding defect of the deletion mutant strain TOH97, that does not express scpB. Thus, the delta12 allele has no effect on the fibronectin binding of recombinant ScpB, recombinant ScpB-phage display fragment, or ScpB expressed by group B streptococci. High-affinity interaction between ScpB and immobilized fibronectin is responsible for the maintenance of the scpB gene in strains lacking C5a peptidase activity

687071

additional information

Streptococcus sp. COH1

-

mutant ScpBdelta, a common natural variant of ScpB, contains a 4-amino-acid deletion that eliminates peptidase activity. High affinity for fibronectin is maintained in ScpBdelta. ScpB and scpBdelta can complement the fibronectin-binding defect of the deletion mutant strain TOH97, that does not express scpB. Thus, the delta12 allele has no effect on the fibronectin binding of recombinant ScpB, recombinant ScpB-phage display fragment, or ScpB expressed by group B streptococci. High-affinity interaction between ScpB and immobilized fibronectin is responsible for the maintenance of the scpB gene in strains lacking C5a peptidase activity

-