Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.108: HtrA2 peptidase

This is an abbreviated version!
For detailed information about HtrA2 peptidase, go to the full flat file.

Word Map on EC 3.4.21.108

Reaction

cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues =

Synonyms

DegP, dOmi/HtrA2, high temperature requirement A serine protease, high temperature requirement A2, high temperature requirement A2 protease, high temperature requirement A2 serine protease, high temperature requirement protein A2, high-temperature requirement factor A2, HtrA protease, HtrA/DegP, HtrA2, HtrA2 protease, HtrA2 serine protease, HtrA2(Omi), HtrA2/Omi, HtrA2/Omi serine protease, Nma11p, Omi, Omi protease, Omi/HtrA2, Omi/HtrA2 protease, Omi/HtrA2 serine protease, pro-apoptotic serine protease, S01.278, serine protease, serine protease HtrA2, serine protease HtrA2/Omi, serine protease Omi/HtrA2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.108 HtrA2 peptidase

Cloned

Cloned on EC 3.4.21.108 - HtrA2 peptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
barely expressed full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21
coexpression of Flag-tagged and Ndelta133 HtrA2 proteins and a myc-epitope-tagged GRIM-19 protein in MCF-7 cells. MCF-7 cells transfected with expression vectors coding for GFP-HtrA2 and GFP-HtrA2-S306A and GRIM-19
-
constructs encoding C-terminal His6-tagged full-length dOmi or truncated mutants are generated using the pET-21a and the pVL-1393 transfer vector, proteins are overexpressed in Escherichia coli BL21DE3 and Sf9 insect cells, respectively
expressed in Escherichia coli (BL21) transformed with pRSET containing cDNA for full-length wild-type Omi or mutant
-
expressed in human embryonic kindney cells and in Escherichia coli
-
expressed in yeast as a bait protein in a two-hybrid assay and by transient transfection of Human embryonic kidney (HEK)-293 cells
-
expression as GST fusions in Escherichia coli BL21
-
expression from pET-15b
-
expression in Escherichia coli
expression in Escherichia coli and MEF cells from mnd2 mouse
-
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli BL21 (DE3) pLysS
-
expression in Escherichia coli BL21 Codon Plus (DE3)-RIL
-
expression of GST-HtrA2 delta 133 proteins in Escherichia coli BL21 from GST-HTRA2 delta 133 WT, S306 and F149D constructs
-
HtrA2/Omi cDNA cloned into pEGFP-C3 at KpnI/XbaI sites. HeLa cells transfected with GFP-fused premature or mature HtrA2/Omi together with or without wild-type and constitutively active Akt. HEK-293 cells transfected with pcDNA3-Myc-HtrA2/Omi together with or without wild-type and constitutively active Akt
-
into pDNA3.1+
into plasmid pEGFPN3 and plasmid pGEX-4T1-FLAG. Expression of GST-HtrA2 fusion protein in BL21 cells and in HEK-293 cells
-
into the pCR-Blunt vector and subsequently into the pAxCAwt cosmid for transfection
-
into the vector pCMV/SPORT
-
into the vectors pCaSpeR-HS and pUASp
into the vectors pUASt and pTMR
overexpression in Escherichia coli BL21 (DE3) and expressed in Saccharomyces cerevisiae EGY48/pLexA-hPag and their mutants
-
overexpression in HeLa cells and HEK-293 cells
-
overexpression of Nma11p in Saccharomyces cerevisiae strain BYa by insertion of a C-terminal GFP-tag to the BIR1ORF
-
pCMV-HtrA2 plasmid encoding full-length HtrA2 with a C-terminal FLAG epitope tag transiently transfected into HEK-293 cells. Plasmid expressing either wild-type or mutant HtrA2 (pCMV-delta133 or pCMV-delta133 (S306A)) transiently transfected with a plasmid encoding full-length APP with a C-terminal Myc tag (pCMV-APP695M) and overexpression in HEK-293 cells
-
PDZ domain
-
recombinantly expressed
-
subcloned into pET28a and propageted in Escherichia coli DH5alpha, expressed in Escherichia coli BL21 (DE3) pLys, overexpression in K269 cells
-
successful expression of full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 as compared to human HtrA2/Omi
-
TAP-tagged HtrA2 stably expressed in HEK-293 cells
-
truncated version, expressed in Escherichia coli
ubiquitin carboxyl-terminal hydrolase L1 and HtrA2 are expressed as GST-fusion proteins in Escherichia coli
-
yes into the ecdysone-inducible eukaryotic expression vector pIND
-