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3.4.21.107: peptidase Do

This is an abbreviated version!
For detailed information about peptidase Do, go to the full flat file.

Word Map on EC 3.4.21.107

Reaction

acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-/-Val =

Synonyms

bacterial PQC factor, BB_0104, BCAL2829, CD630_32840, Deg1, DEG2, DEG5, DEG7, DEG8, Deg9, DegP, DegP protease, DegP/HtrA, DegQ, DegS, DepP9, Do, Do protease, HhoA, HhoB, high temperature requirement A, high temperature requirement A protease, high temperature requirement A1, high temperature requirement factor A, high-temperature requirement A, high-temperature requirement A protease, high-temperature requirement A-1, high-temperature requirement A1, high-temperature requirement A1 protease, high-temperature requirement factor A, HtrA, HtrA (DegP) protease, HtrA heat shock protease, HtrA protease, HTRA serine peptidase 1, HtrA-like protease, HtrA/DegP, HtrA1, HtrA2, HtrA3, HTRA4, MAL8P1.126, More, MucD, Nma111p, Omi/HtrA protease orthologue Ynm3p, protease do, protease Do-like 5, protease Do-like 8, PRSS11, S01.273, serine protease, serine protease HtrA, serine protease HtrA1, YNL123w

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.107 peptidase Do

Purification

Purification on EC 3.4.21.107 - peptidase Do

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
90% purity
-
by Ni affinity chromatography
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glutathione-Sepharose bead chromatography
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HisTrap column chromatography, gel filtration
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near homogeneity
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Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and His GraviTrap affinity column chromatography
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recombinant enzyme. Comparison of the protein from Helicobacter pylori strains 26695, J99 and N6
recombinant His-tagged HtrA1 lacking the N-terminal insulin-like growth factor-binding protein and serine protease inhibitor domain from Escherichia coli by affinity chromatography
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recombinant HtrA S210A mutant from overproducing strain K38
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recombinant HtrA1 from 293-EBNA cell culture medium partially by ultrafiltration and dialysis
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using Ni-NTA chromatography
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wild-type, htrA22 and htrA63 mutant
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wild-type, S210A and H105R mutant
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