3.4.21.106: hepsin

peptide sequence from trask

Abz-RAARVVGGY(3-NO2) + H2O

Abz-RAAR + VVGGY(3-NO2)

Abz-RKRRGSRGY(3-NO2) + H2O

Abz-RKRR + GSRGY(3-NO2)

peptide sequence from filaggrin

Abz-RLARVVGGY(3-NO2) + H2O

Abz-RLAR + VVGGY(3-NO2)

Abz-RQARAVGGY(3-NO2) + H2O

Abz-RQAR + AVGGY(3-NO2)

Abz-RQARVVGGY(3-NO2) + H2O

Abz-RQAR + VVGGY(3-NO2)

2 entries

Abz-RQARYVGGY(3-NO2) + H2O

Abz-RQAR + YVGGY(3-NO2)

Abz-RQLRVVGGY(3-NO2) + H2O

Abz-RQLR + VVGGY(3-NO2)

Abz-RQRRALEKY(3-NO2) + H2O

Abz-RQRR + ALEKY(3-NO2)

peptide sequence from alphaEbeta7 integrin

Abz-RQRRVVGGY(3-NO2) + H2O

Abz-RQRR + VVGGY(3-NO2)

Abz-RQYRVVGGY(3-NO2) + H2O

Abz-RQYR + VVGGY(3-NO2)

Abz-RRARVVGGY(3-NO2) + H2O

Abz-RRAR + VVGGY(3-NO2)

Abz-SKGRSLIGY(3-NO2) + H2O

Abz-SKGR + SLIGY(3-NO2)

peptide sequence from PAR-2

Abz-SKLRVVGGY(3-NO2) + H2O

Abz-SKLR + VVGGY(3-NO2)

peptide sequence from proMSP-1

acetyl-DQLR-7-amido-4-methylcoumarin + H2O

acetyl-KKTR-7-amido-4-methylcoumarin + H2O

acetyl-KQLR-7-amido-4-methylcoumarin + H2O

most active tetrapeptide substrate

acetyl-PVDR-7-amido-4-methylcoumarin + H2O

least active tetrapeptide substrate

blood clotting factor IX + H2O

active blood clotting factor IX

blood clotting factor VII + H2O

active blood clotting factor VII

blood clotting factor XII + H2O

active blood clotting factor XII

Boc-Gln-Ala-Arg-AMC + H2O

Boc-QAR-7-amido-4-methylcoumarin + H2O

Boc-QAR + 7-amino-4-methylcoumarin

754402

epidermal growth factor receptor + H2O

hepsin cleavage of epidermal growth factor receptor is not dependent on receptor tyrosine phosphorylation

709925

factor X + H2O

activates factor X in the presence of factor VII

651852

Glu-Pro-Arg-4-nitroanilide + H2O

653908

hepatocyte growth factor precursor + H2O

3 entries

L-Asp-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O

L-Asp-L-Ala-L-Ala-L-Arg + 4-nitroaniline

717265

L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg-4-nitroanilide + H2O

L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg + 4-nitroaniline

717265

laminin-332 + H2O

by Western blotting and mass spectrometry, it is shown that hepsin cleaves the beta3 chain of rat Laminin-332. N-terminal sequencing identifies the cleavage site at beta3 Arg245, in a sequence context (SQLR245 cleavage LQGSCFC) conserved among species and in remarkable agreement with reported consensus target sequences for hepsin activity

698824

microsomal glutathione S-transferase 1 + H2O

active microsomal glutathione S-transferase

hepsin seems to activate through microsomal glutathione S-transferase 1 dimer formation

N-acetyl-KQLR-7-amido-4-methylcoumarin + H2O

N-acetyl-KQLR + 7-amino-4-methylcoumarin

717225

N-acetyl-L-Lys-L-Arg-L-Leu-L-Arg-7-amido-4-carbamoylmethylcoumarin + H2O

707437

N-benzoyl-Ile-Glu-Phe-Ser-Arg-4-nitroanilide + H2O

3 entries

N-benzoyl-Leu-Ser-Arg-4-nitroanilide + H2O

4 entries

N-benzoyl-Phe-Val-Arg-4-nitroanilide + H2O

3 entries

N-benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide + H2O

N-benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin

weak substrate

N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O

N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin

weak substrate

pro-hepatocyte growth factor + H2O

active hepatocyte growth factor

2 entries

pro-macrophage-stimulating protein + H2O

the cleavage site is between Arg(483) and Val(484). At least 50% of the substrate is processed within 1 h at a hepsin concentration of 2.4 nM and at a molar enzyme to substrate ratio of 1:500

718105

pro-urokinase + H2O

active urokinase

pro-urokinase-type plasminogen activator + H2O

active high molecular weight urokinase-type plasminogen activator

cleavage at the Lys158-Ile159 (P1-P1') peptide bond

687522

prostasin pro-peptide + H2O

prostasin

hepsin activates prostasin, cleavage occurs at Arg44

Protein + H2O

S2366 + H2O

single-chain hepatocyte growth factor + H2O

two-chain hepatocyte growth factor

efficiently converted by soluble form of hepsin comprising the entire extracellular domain

673609

Suc-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O

2% active with the purifed enzyme

t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O

12% active with the purifed enzyme

t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumaryl-7-amide + H2O

t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin

753485

t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O

100% active with the purifed enzyme

t-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumaryl-7-amide + H2O

58% active with the purifed enzyme

t-butyloxycarbonyl-Glu-Ala-Arg-4-methylcoumaryl-7-amide + H2O

41% active with the purifed enzyme

t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O

8% active with the purifed enzyme

t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O

25% active with the purifed enzyme

t-butyloxycarbonyl-Leu-Lys-Arg-4-methylcoumaryl-7-amide + H2O

19% active with the purifed enzyme

t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O

13% active with the purifed enzyme

t-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O

20% active with the purifed enzyme

tert-butyloxycarbonyl-Gln-Ala-Arg 4-methylcoumarin 7-amide + H2O

tert-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin

weak substrate

tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O

tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin

tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O

tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin

weak substrate

Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O

9% active with the purifed enzyme

zymogen factor VII + H2O

factor VIIa