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3.4.21.106: hepsin

This is an abbreviated version!
For detailed information about hepsin, go to the full flat file.

Word Map on EC 3.4.21.106

Reaction

cleavage after basic amino-acid residues, with Arg strongly preferred to Lys =

Synonyms

hepsin, S01.224, TMPRSS1, transmembrane serine protease 1, type II transmembrane serine protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.106 hepsin

Application

Application on EC 3.4.21.106 - hepsin

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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the aim of this study is to establish a cell line that directly secrets rFVIIa into cell culture medium: Factor VII and hepsin cDNAs are isolated from HepG2 cell line and cloned into pcDNA3-1 vector. The constructs are co-trasfected to CHO cell line. A cell line that permanently expresses recombinant factor VII (rFVII) and hepsin is established. FVIIa protein is secreted to medium of CHO cells co-transfected with pcNDA3-1-FVII and pcNDA3-1-hepsin. A three- to fourfold decrease in clotting time is observed when human FVII-depleted plasma is used in combination with human thromboplastin in the presence of rFVII, confirming the biological activity of rFVII. A cell line is established expressing FVIIa using genetic engineering methods
medicine