3.4.21.104: mannan-binding lectin-associated serine protease-2

This is an abbreviated version!
For detailed information about mannan-binding lectin-associated serine protease-2, go to the full flat file.

Reaction

Selective cleavage after Arg223 in complement component C2 (-Ser-Leu-Gly-Arg-/-Lys-Ile-Gln-Ile) and after Arg76 in complement component C4 (-Gly-Leu-Gln-Arg-/-Ala-Leu-Glu-Ile) =

Synonyms

CCP1-CCP2-SP, Mannan-binding lectin associated serine protease-2, mannan-binding lectin-associated serine protease, mannan-binding lectin-associated serine protease 2, mannan-binding lectin-associated serine protease-2, mannose-binding lectin-associated serine protease 2, mannose-binding lectin-associated serine protease-2, mannose-binding lectin-associated-serine protease-2, Map19, MASP, MASP-2, MASP-2A, MASP-2K, MASP2, MBL-associated serine protease, MBL-associated serine protease 2, MBL-associated serine protease-2, MBL-associated-serine protease-2, MBL-MASP, MBL/ficolin-associated serine protease, MBP-associated serine protease, MBP-associated serine protease 2, MBP-associated serine protease-2, S01.229

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.104 mannan-binding lectin-associated serine protease-2

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Results	in table
21	Activating Compound
11	AA Sequence
6	Application
1	CAS Registry Number
31	Cloned(Commentary)
8	Crystallization (Commentary)
309	Disease/ Diagnostics
11	General Information
1	General Stability
32	Inhibitors
7	Ki Value [mM]
26	KM Value [mM]
10	Localization
15	Metals/Ions
28	Molecular Weight [Da]
70	Natural Substrates/ Products (Substrates)
70	Organism
10	PDB ID
11	pH Optimum
1	pH Range
1	pH Stability
2	pI Value
12	Posttranslational Modification
30	Protein Variants
18	Purification (Commentary)
5	Reaction
18	Reaction Type
69	Reference
2	Renatured (Commentary)
40	Source Tissue
5	Specific Activity [micromol/min/mg]
3	Storage Stability
158	Substrates and Products (Substrate)
28	Subunits
110	Synonyms
10	Temperature Optimum [°C]
2	Temperature Stability [°C]
27	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.104 - mannan-binding lectin-associated serine protease-2

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

chimeric proteins C1s(MASP-2CCP1/2) and C1s(MASP-2SP)

Homo sapiens

O00187

665686

from serum by sequential affinity chromatography, separation from serum mannose-binding protein MBP in presence of EDTA

Homo sapiens

-

659609

mannan-agarose column chromatography

Homo sapiens

-

708697

MASP-2K mutant, inhibited in autoproteolytic activation, by affinity chromatography on an myelin basic protein-resin

Rattus norvegicus

-

659350

mature protein and truncated fragments

Homo sapiens

-

652189

MBL-derivatized Sepharose column chromatography

Homo sapiens

-

680169

Ni-Sepharose column chromatography

Rattus norvegicus

-

709084

Q-Sepharose column chromatography

Homo sapiens

-

718122

recombinant catalytic fragment encompassing the second complement control protein module and the serine protease domain, i.e. MASP-2-CCP2-SP, from Escherichia coli strain BL21(DE3)

Homo sapiens

Q92876

659718

secreted recombinant catalytically active and inactive wild-type and mutant MASP-2 zymogens from CHO cell culture medium

Rattus norvegicus

-

683739

-

Homo sapiens

-

652143, 652677, 652683, 652684, 653331, 681205, 717934

-

Homo sapiens

O00187

665655