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3.4.21.102: C-terminal processing peptidase

This is an abbreviated version!
For detailed information about C-terminal processing peptidase, go to the full flat file.

Word Map on EC 3.4.21.102

Reaction

the enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-/-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala. In the plant chloroplast, the enzyme removes the C-terminal extension of the D1 polypeptide of photosystem II =

Synonyms

At4g17740, C-terminal processing peptidase, C-terminal processing protease, C-terminal protease, carboxy terminal-processing proteinase, carboxyl terminal processing protease of D1 protein, carboxyl-terminal processing protease, carboxyl-terminal protease, carboxyl-terminal-processing peptidase 2, carboxyterminal processing protease, carboxyterminal processing protease of D1 protein, chloroplast processing enzyme, chloroplast protein precursor processing proteinase, CPE, CtpA, CtpA gene product, CtpB, D1 preprotein-processing proteinase, D1 protein-processing enzyme, D1P, More, PA5734, photosystem D1 protein precursor carboxyl-terminal processing protease, photosystem II D1 C-terminal processing protease, photosystem II D1 protein, photosystem II D1 protein processing proteinase, photosystem II protein D1, photosystem II protein D1 2, PrC, Prc protease, PRC protein, processing peptidase, protease Re, proteinase CtpA, proteinase, chloroplast protein precursor-processing, PsbA4, PXO_04290, SPP, stromal processing peptidase, tail-specific protease, thylakoid protein precursor processing peptidase, Tsp protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.102 C-terminal processing peptidase

Cloned

Cloned on EC 3.4.21.102 - C-terminal processing peptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
creation of non functional mutants by insertional mutagenesis, strains carrying the mutation are growth deficient, and more sensitive to desiccation and detergents than the wild type strain
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli S17.1 cells
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expression in Escherichia coli
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expression in the non-nitrogen-fixing cyanobacterium Synechocystis sp. PCC 6803
expression of the His-tagged enzyme in Escherichia coli strains BL21(DE3)pLysS or B834(DE3)
gene ctpA, DNA and amino acid sequence determination and analysis, genomic organization and structure, complementation of the mutant strain 1330DELTActpa by expression of wild-type gene ctpA
gene ctpA, expression of His-tagged CtpA in Escherichia coli mainly in inclusion bodies
gene prc from three strains, DNA and amino acid sequence determination and analysis, prc is located in a gene cluster encoding an extreme stress response system composed of anti-sigma factor MucA, negative regulator MucB, and ECF alternative sigma factor AlgU, regulation, overview
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ORFs SLL2008 and SLL2009 encoding the C- and N-terminal enzyme part, respectively, operon organization
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overexpressed in Arabidopsis thaliana, expressed as His-tag fusion protein in Escherichia coli
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overexpression in Escherichia coli
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sequence analysis
single gene, sequence analysis, expression analysis
O48870