3.4.21.1: chymotrypsin

This is an abbreviated version!
For detailed information about chymotrypsin, go to the full flat file.

Reaction

Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/- =

Synonyms

4CHA, Alcalase, alpha chymar, alpha chymotrypsin, alpha-Chy, alpha-chymar ophth, alpha-chymotrypsin, alpha-chymotrypsin A, alpha-CT, avazyme, bovine alpha-chymotrypsin, caldecrin, cationic chymotrypsin, cellulomonadin, CHT, Chtp, Chtr1, Chtr2, Chtr3, Chtr4, ChTRP, CHY1, CHY20, chymar, chymotest, chymotrypsin, chymotrypsin A, chymotrypsin B, chymotrypsin C, chymotrypsin C1, chymotrypsin I, chymotrypsin II, chymotrypsin isoform Kh1, chymotrypsin isoform Kh2, chymotrypsin isoform Kh3, chymotrypsin-B, CTRA, Ctrb, EC 3.4.4.5, EC 3.4.4.6, enzeon, LBCP, lysosomal Bid cleavage protease, PEG-alpha-chymotrypsin, PEG-modified alpha-chymotrypsin, quimar, quimotrase, serine protease

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.1 chymotrypsin

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Results	in table
24	Activating Compound
103	AA Sequence
19	Application
1	CAS Registry Number
7	Cloned(Commentary)
14	Crystallization (Commentary)
1998	Disease/ Diagnostics
5	General Information
11	General Stability
45	IC50 Value
335	Inhibitors
44	kcat/KM [mM/s]
115	Ki Value [mM]
189	KM Value [mM]
5	Localization
11	Metals/Ions
35	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organic Solvent Stability
199	Organism
245	PDB ID
31	pH Optimum
16	pH Range
17	pH Stability
10	pI Value
8	Posttranslational Modification
5	Protein Variants
32	Purification (Commentary)
2	Reaction
1	Reaction Type
172	Reference
113	Source Tissue
33	Specific Activity [micromol/min/mg]
6	Storage Stability
250	Substrates and Products (Substrate)
19	Subunits
148	Synonyms
16	Temperature Optimum [°C]
11	Temperature Range [°C]
27	Temperature Stability [°C]
187	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.4.21.1 - chymotrypsin

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

25

3 entries

25 - 55

Sardinops sagax

-

activity quickly decreases at temperatures above the optimum, the relative activity of the enzyme at 25°C is 60% of maximum activity, the enzyme is labile at high temperatures (more than 55°C) and stabilized at less than 30°C

679953

25 - 60

Rattus norvegicus

-

rapid inactivation above 60°C

651113

30

Penaeus californiensis

U5KGX4

120 min, 20-40% loss of activity

731855

35

Panulirus interruptus

-

60 min, about 30% loss of activity

754581

35 - 55

Bos taurus

-

approx. 50% loss of activity after 10 min at 50°C, complete loss of activity after 10 min at 55°C

650666

40

2 entries

45

3 entries

49.5

Bos taurus

-

melting temperature

678725

50

Penaeus californiensis

U5KGX4

30 min, complete loss of activity

731855

52.8

Bos taurus

-

melting temperature

680206

55

3 entries

65

Dosidicus gigas

-

60 min, no loss of activity

753641

75 - 85

Bos taurus

-

almost no activity is retained when the incubation temperature is above 75°C, the activity of the nanogel-bound alpha-CT still has a residual activity of 88.7% at 85°C

681488

80

Bos taurus

P00766

the entrapment of alpha-chymotrypsin within 70-40 nm liposomes formed from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine leads to a remarkable increase in the thermal stability of the enzyme. Heating aqueous suspensions of alpha-chymotrypsin-containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes to 80°C for 30 minutes results in partial enzyme inactivation, whereas the same treatment of aqueous solutions of free enzyme inactivates the enzyme completely. The stabilizing effect of enzyme confinement in the attoliter volumes of the liposomes increases with decreasing numbers of alpha-chymotrypsin molecules per liposome

753261

95

Thermotoga maritima

Q9WZP2

half-life: 36 min

747755

additional information

4 entries

25

Bos taurus

-

retaining around 85% of the initial activity after 2 h

667470

25

Dosidicus gigas

-

60 min, about 60% loss of activity

753641

25

Panulirus interruptus

-

60 min, about 20% loss of activity

754581

40

Bos taurus

-

is inactivated at 60°C

708549

40

Sardinops caeruleus

-

chymotrypsin I is completely inactivated at 55°C and chymotrypsin II at 50°C

708549

45

Dosidicus gigas

-

60 min, about 50% loss of activity

753641

45

Panulirus interruptus

-

60 min, about 40% loss of activity

754581

45

Pterygoplichthys disjunctivus

-

stable below

731829

55

Dosidicus gigas

-

60 min, about 5% loss of activity

753641

55

Panulirus interruptus

-

20 min, about 80% loss of activity. 40 min, complete loss of activity

754581

55

Pterygoplichthys disjunctivus

-

labile above

731829

additional information

Bos taurus

-

thermostability of alpha-chymotrypsin is enhanced by about 4-6°C after modification with mono-6-formyl-beta-cyclodextrin and mono-6-succinyl-6-deoxy-beta-cyclodextrin

650666

additional information

Bos taurus

-

native bovine chymotrypsin rapidly loses activity when incubated at 55°C. Glycosylated chymotrypsin has greatly increased thermostability compared to the native enzyme, after incubation at 50°C and pH 8.0, glycosylated bovine chymotrypsin retains 45% of its original activity and the native enzyme is inactivated

691416

additional information

Bos taurus

-

significant improvement in thermal stability of chymotrypsin is achieved after the covalent attachment of the enzyme on chitosan. The half-life of immobilized chymotrypsin can be increased from 0.57 h, at 55°C, to 7.8 h, at 65°C

691186

additional information

Bos taurus

-

thermodynamic unfolding experiments reveal that PEGylation increases the thermal transition temperature (Tm) of alpha-CT by up to 6°C and the free energy of unfolding [DELTAGU (25°C)] by up to 5 kcal/mol

691412